Oral and Poster Abstracts

had greater trans11-18:1 percentage in milk fat (2.39%) this response
resulted in lower milk fat (3.47%). In the farm C the percentage of CLA,
the ratio of trans11-18:1 to CLA and the ratio (6:3) of FA were very
beneficial according to human health demands. Milk fat content was
negatively correlated to trans11-18:1 percentage (r=-0.93) and to CLA in
the milk fat (r=-0.85). Our data suggest that rate of ruminal degradation of
NDF influences the milk fat content. Results showed that there was a
negative correlation between milk fat content and degradation rate of
NDF (r=-0.91). It can be assumed, that even suboptimal fiber levels in
TMR containing slower degradable fiber may be sufficient to maintain
proper rumen fermentation and level of milk fat synthesis. This finding
suggests that in the TMR of cows not only the quantity of fiber fractions
(NDF, ADF) has to be determined, but even their degradation rate should
be taken into consideration.
538 The Effect of Antioxidant and Omega-3 fatty acid
Supplement on Metabolic Status and Reproductive
Performance in Postpartum Dairy Cows
F. Tóth 1 , Gy. Gábor 1 , O. Balogh 1 , G. Koppány 2 , H. Fébel 1
1
Research Institute for Animal Breeding and Nutrition, Herceghalom,
Hungary
2
Vitafort Ltd., Dabas, Hungary
The objective of this study was to investigate the effect of different
supplements on metabolic status, the onset of cyclic ovarian and the
rate of re-conception function in the postpartum (pp.) period. The study
was carried out on 66 pp. Holstein cows. The cows were fed the same
total mixed ration in dry period and 5-7 pp. days were divided into the
control (C) (n=16) and the experimental (E) group (n=50). The feed of
E was supplemented with extra beta carotene, vitamin E, inactivated
yeast, selenium and microelement chelates until 50 pp. d.; from 50 pp.
days onwards the supplement was only 200 g grinded linseed (W3 fatty
acid supplement). The average milk yield of E cows was 3 kg/day
higher than C cows from 8 th to 120 th lactation day. The plasma level of
BHB was higher in the E group. While both groups showed elevated
NEFA level on day 30, only C group had increased NEFA
concentration on day 50. Plasma concentration of urea was lower in the
E group on day 90 and 120 pp. Serum level of carotene in the E group
was higher during the whole experiment. P4 concentrations between
Days 18 and 39 after AI were greater for cows fed linseed
supplementation than the control cows. According to the P4 profile
more than 80 % of the E group had cyclic ovarian activity versus only
62.5% of the C group. Although, the conception at first AI (41.2 vs.
57.1 %) and the overall proportion pregnant (47.1 vs. 71.4 %) was
lower in the E group. The hypothesis that feeding linseed would
increase conception rate of dairy cows was not supported by the present
experiment. The higher P4 level of E cows means the positive effect of
omega 3 fatty acids and may also have contributed to decrease embryo
mortality. The applied supplements effected elevated milk production,
but this higher production led to a negative energy balance (NEB)
which is confirmed by elevated levels of NEFA and BHB. This
negative effect of NEB is presumably connected to lower fertility
performance.
This work was supported by the project of OMFB-0121
Key words: conception rate, omega-3 fatty acid, antioxidant,
metabolic status
539 Use of hypertonic and isotonic saline solution and furosemide
for the treatment of the ammonia poisoning in cattle
S. Kitamura 1,2,3 , A. Antonelli 1 , R. Barreto Júnior 1 , C. Mori 1 ,
M. Sucupira 1 , B. Caputi 1 , E. Ortolani 1
1
Faculdade de Medicina Veterinaria da Universidade de Sao Paulo,
Clinica Medica, Sao Paulo, Brazil
2
Universidade Anhembi Morumbi, Clinica Medica, Sao Paulo, Brazil
3
Universidade Paulista - UNIP, Instituto de Ciencias da Saude, Sao
Paulo, Brazil
To test the efficiency of some treatments for ammonia poisoning in
cattle 25 steers, never fed non-protein nitrogen before, were used.
Ammonium chloride solution was infused intravenously (iv) only
once in each steer until the onset of convulsive episode, and then the
infusion was immediately stopped. Thereafter, the animals were
randomly distributed in one of the five different groups, as follows:
1) control (infusion 300 mL isotonic saline solution - ISS); 2)
infusion (iv) of ISS 30 mL/kg BW throughout the following 4 h and
administration by stomach tube of 4 L water. (W); 3) same as group
2, plus a single dose 2 mg furosemide/kg BW (iv) (F); 4) injection of
5 mL/kg BW hypertonic saline solution (HSS) (7.2 %) (iv)
throughout the first 30 min, followed by 20 mL/ kg BW of ISS and 4
L W.; 5) Same as group 4 and a single dose of F. For the next 4 h
after the convulsion several clinical variables and the blood
concentration of ammonia, glucose, lactate, urea, creatinine, sodium
and potassium, hemogasometric profile, urine global volume and
urinary excretion of ammonium, urea and creatinine were followed.
The clinical picture seen just before the treatment was classical and
caused a characteristic systemic metabolic acidosis, compensated by
reduction in the pCO 2, hyperlactemia and hyperglycemia. At the
convulsion the ammonia blood levels were high and similar among
the groups, but at the 120 th min the animals treated with HSS had
lower values than the control. The HSS infusion caused, during the
first 30 min. a marked reduction in the packet cell volume and the
heart rate. On the other hand, furosemide provoked an increase in the
heart rate and neither prevented the onset of lung edema, nor an
increase in the total excretion of urine. Cattle treated with HSS stood
up and had the return of the rumen movements earlier than other
groups. The therapy with HSS still increased the urinary volume and
the total urinary excretion of urea and ammonium during the critical
1 st h of treatment, while the use of ISS had intermediate results. The
efficiency of the treatment with HSS or ISS as seen by some
variables of clinical improvement was better than the control group.
The higher the urinary urea excretion was, the greater the ammonia
elimination became. Although with lower efficiency as seen in the
treatment with HSS, the ISS caused an improvement in the clinical
picture and at the end of the experiment generated an adequate
ammonia detoxification.
Key words: ammonia intoxication (treatment), hypertonic,
furosemide, cattle
540 Environmental Low Phosphorus Induced the Erythrocytic
Membrane Damage: a Pathogenesis of Endemic
Haemoglobinuria in Dairy Cattle
JL. Li, Q. Si, FQ. Shi, SW. Xu
Northeast Agricultural University, College of Veterinary Medicine,
Harbin, China
Objectives: Dairy cattle could display haemoglobinuria when
phosphorus was deficient in food, especially in environmental low
phosphorus in some regions. Endemic haemoglobinuria in dairy cattle
usually had been detected in some regions of Heilongjiang province in
china. However, the reason of the endemic haemoglobinuria remians
unclear. The aim of this study is to illustrate the pathogenesis of
endemic haemoglobinuria.
Materials and Methods: In this study, we tested the content of
phosphorus in soil-grass and serum of 15 cows with obvious clinical
symptom in Daqing and Anda regions of Heilongjiang province in
April-July and October, respectively. In addition, we also detected
Lipid composition, fluidity, viscosity and activity of ATPase of
erythrocytic membrane of the 15 cows as well as osmotic fragility of
the erythrocytes and blood routine.
Results: Our results indicated that the content of soil and grass
phosphorus was significantly lower (P