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214 Cystic Ovarian Diseases in Dairy Cows: Epidural GnRH and Calcium/Naloxone Administration M. Cinone 1 , G. Minoia 1 , D. Robbe 1 , A. Guaricci 1 , D. Coronati 3 , M. Mutinati 1 , A. Rizzo 1 1 Faculty of Veterinary Medicine of Bari, Department of Animal Production, Valenzano (BA), Italy 2 University of Teramo, Clinical Veterinary Scieces, teramo, Italy 3 CIRIO Agricola Farm Animals, Veterinarian, Caserta, Italy Objectives: Cystic ovarian diseases (COD) occur in postpartum dairy cows and are characterized by the presence of anovulatory ovarian structures. Their etiopathogenesis is multifactorial and related to the asynchrony in the induction of LH receptors on the granulosa and theca cells during follicular maturation or to their desensitization. Endogenous opioid peptides (EOP) have been documented in several parts of the reproductive tract and are involved in the control of the stress-induced changes in LH secretion. The possibility of an intragonadal local opioidergic control cannot be excluded because the presence of ‚-endorphins (‚end) has been detected in the ovary of some mammals. The present study was designed 1) to measure -end concentrations in bovine cystic fluid; 2) to evaluate the impact of the epidural administration of GnRH and calcium/naloxone (Ca/Nx) on EOP removal. Materials and methods: The experimental study was performed on 45 Friesian cows (60 d. postpartum) affected by COD and on 5 oestrus cows. Animals were assigned to 4 groups: A- 30 cows undergoing an epidural treatment with 3ml of Ca/Nx (naloxone hydrochloride 0.6mg dissolved in 20% calcium gluconate) and 2ml of GnRH (25ng/ml lecirelina ® ), twice, in an interval of 21 d; B- 10 cows receiving 5ml of 0.9% NaCl solution, epidural via; C- 5 cows in which cystic fluid was aspired in vivo; D- 5 oestrus cows in which the fluid from the preovulatory dominant follicle was aspired. Oestrus occurred in all cows, thus AI was performed. Pregnancy was confirmed 30 d. after AI. 15/30 cows with COD showed oestrus in the following 18±3 d. after GnRH+Ca/Nx treatment. Conception rate was 66% (10/15 cows). Plasma ‚-end concentration increased (1.9±1ng/ml) after the 1st administration and decreased up to 0.5±0.2ng/ml (p
lactating Holstein Friesian cows and six Japanese Black beef cows had been ovariectomized before the experiment. The devices were inserted into the vagina 2 to 3 weeks after the ovariectomy and removed 12 days after the insertion. Blood was collected via the tail venepuncture once a day at 8.00 a.m. from the day before insertion to 15 days after the insertion. On the day of insertion plasma samples were also collected at 0, 1, 2, 4, 6, and 8 h after the insertion. Plasma P4 concentrations were determined by ELISA. The experiment was repeated in some animals at 8 to 10 d intervals. The plasma P4 concentrations in cows of the DIB group (n=7) increased to 2.6 ± 1.0 ng/ml at 1h, and to 4.6 ± 1.4 ng/ml at 2 h and, thereafter, remained above 1.0 ng/ml until the 12th day. Likewise, plasma P4 concentrations in cow of CIDR group (n=7) rose to 1.6 ± 0.6 ng/ml at 1h, and to 2.7 ± 1.3 ng/ml at 2 h after the insertion. A high P4 concentration above 1.0 ng/ml was maintained until the day of removal. There was no significant difference in the plasma P4 profiles between the dairy and beef cows. Thus, the new intravaginal device is shown to be effective to increase plasma P4 concentrations within a few hours after insertion and to maintain the elevated levels during a treatment period of 12 d in cows, which is well comparable with the effectiveness of CIDR. Key words: DIB, CIDR, progesterone device, Holstein Friesian, Japanese Black 255 Control of Oestrus with a Progesterone Intravaginal Device (Prid ® ): Comparison of 2 Insertion Durations S. Floc’H 1 , F. Deletang 2 , S. Freret 3 , C. Ponsart 4 , D. Remmy 5 1 CEVA santé Animale, Livestock Business Unit, Libourne, France 2 Animal Reproduction, La Rochelle, France 3 INRA, Animal Reproduction, Tours, France, France 4 UNCEIA, R&D, Maisons-Alfort, France 5 CEVA Santé Animale, Ruminants, Libourne, France The objective of this study was to optimise the efficacy of PRID by comparing 2 insertion durations, 7 and 9 days, in term of pregnancy rate after a single fixed time artificial insemination. This study was conducted during 2 years: firstly in beef cattle (n=748) and secondly in dairy ccattle (n=498). In beef cattle only, blood was sampled twice before treatment for determination of plasma progesterone to check cyclicity: 66.2 % of females were defined as cyclic at the beginning of the treatment. Pregnancy was checked by transrectal ultrasonography 35 days post insemination or by pregnancy Specific Protein B (PSPB) dosage by RIA 45 days post insemination or by rectal palpation. Treatment protocol was : PRID insertion for 7 or 9 days, PgF2 injection 24h before removal of PRID, equine Chorionic Gonadotropin (eCG) was injected at the time of removal : from 400 to 600 IU according to breed and physiological status. Cows were bred once 56h after PRID removal. The global pregnancy rate was 60.1 % (n=749/1246). The treatment duration influenced this pregnancy rate as it tended to be higher (p = 0.056) in the 9 days group (62.8 %) vs. 7 days group (57.5 %). The effect of calving rank was observed with a higher pregnancy rate in heifers (65 %) than in cows (53.2 %). To compare the 2 groups, higher differences in pregnancy rates in the 9 vs. 7 days group were observed in heifers in beef cattle (78.5 % vs. 68.5 %) and in cows in dairy cattle (42.9 % vs. 33.3 %). The effect of body condition score (BCS) was observed only in dairy cows with a tendency (p = 0.09) for higher pregnancy rate in animals with a BCS of 3 vs. those with a BCS 2.5 or 3.5 (54.7 % vs. 45 % respectively). Further, higher pregnancy rates were observed in the 9 vs. 7 days group in dairy cows with BCS 2.5 (50 % vs. 41.9 %). Regarding cyclicity in beef cattle, pregnancy rate was significantly higher (p = 0.0002) in cycling (71.2 %) vs. non cycling animals (57.4 %). The highest difference between 9 and 7 days group was observed in non cycling females (64.3 % and 50 % resp.). Results of this study showed better pregnancy rates with a 9 vs. 7 days insertion of PRID (62.8 % vs. 57.5 % respectively), it was then concluded that a 9 days insertion tends to improve fertility (p = 0.056), more particularly in non cycling vs. cycling animals and in dairy cattle in cows vs. heifers and in animals with BCS < 3. Key words: progesterone, PRID, treatment duration 256 Effect of Progesterone Supplementation post Conception on Embryo Survival Rate in Heifers M. Beltman 1 , P. Lonergan 2 , J. Roche 2 , M. Diskin 3 , M. Crowe 2 1 University College Dublin, Unit of Herd and Veterinary Public Health, Dublin, Ireland 2 University College Dublin, Animal and Veterinary Science, Dublin, Ireland 3 Teagasc, Animal Production Research Centre, Athenry, Ireland Progesterone (P4) is essential for establishment and maintenance of pregnancy in mammals. The objective of this study was to examine the effect of elevating P4 during the first week post conception on embryo survival. Oestrus was synchronized in beef cross heifers (n=197, ~2-years old) using two injections of prostaglandin F 2a (PG, Prosolvin, Intervet Irl.) 9 days apart. The heifers were checked for oestrus 4x daily commencing 24h after the second PG and were inseminated 12-18 h after oestrus onset (=day 0). Inseminated heifers were randomly assigned to 1 of 3 groups: (i) Control, n=69; (ii) P4 supplementation using a Controlled Internal Drug Device (CIDR 1.9g, Pfizer UK) from days 3 to 6.5, n=64; or (iii) P4 (CIDR) from days 4.5 to 8, n=64. Body condition (BCS) and locomotion scores (scale of 1-5) were recorded for all animals. Animals with a locomotion score 4 (very lame) were excluded. Pregnancy was determined at slaughter on day 25. Conceptus length and weight were recorded and the corpora lutea (CL) of all pregnant animals were weighed. Embryo survival rates were compared by Chi-square test and the relationships between BCS, locomotion score, P4 levels and embryo survival rates were analysed using regression analysis. Supplementation with exogenous P4 increased (P
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130. évfolyam Különszám II. Vol
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The World Buiatrics Congress (WBC)
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lood and liver tissue. Absolute vit
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RNS causes increased lipid peroxida
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An experiment was conducted to stud
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2 Hungarian Institute of Agricultur
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and plasma HCO 3 - increased from H
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Materials and Methods: Serum sample
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vitamin A stores in liver, the hypo
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andomly assigned to one of five tre
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observed in group II of the animals
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The aim of the monitoring was to co
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health group (HG) categories for an
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lactating cows is sparse. Lotan and
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491 Effect of Acarbose on Milk Yiel
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499 Secondary Hepatogenous Photosen
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they all died in a two to three day
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and HCO 3 level. The concentration
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amount of saponins was observed in
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Water was offered ad-libitum. Feed
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Se, I and Co) more than 500 blood s
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541 Variability of Blood Profile an
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evaluated, highly significantly gre
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we measured sensitivity of the enti
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were 0.17; 0.13 °C (Max.: 0.73). D
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calving pregnancy period were the b
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2 University of Nottingham, Departm
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animals) was treated with 5 mg enro
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In dairying, good management practi
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Somatic cell count (SCC) is one of
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This investigation was run in some
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eproductive data showed that cows f
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(3.6%) and others (Bacillus spp., N
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602 Coagulase Gene Polymorphism of
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2 Institute of Veterinary Bacteriol
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an important part of the pathogens
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4 Universidade de Sao Paulo, Clinic
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incubated for one hour at 37 oC. Af
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In Belgium, an increasing amount of
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Conclusion: It can be concluded tha
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calves recovered after 4 to 10 days
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disease. Thus losses were estimated
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635 Prevalence of the Pulmonary Les
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Conclusions The sanitation program
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Italy. After the sample examination
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Results: The cows sera were collect
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(Mb) as co-infecting agent is becom
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distributed over 13 different fatte
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main infection sources to the urban
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686 Characterisation of Cattle Orig
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using EPFC. The intradermal tubercu
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three cattle farms each. Leptospiro
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(cELISA) only to definitively confi
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1 Islamic Azad University of Shahre
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Financial support: CNPq, CAPES, FIN
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731 Detection and Investigation on
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necropsied, tissue samples were tak
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aseline values. The mean HCO 3, PCO
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immunization with either the curren
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decrease in animal aggregation in p
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747 Effect of Dietary Supplementati
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755 Study of Pulmonary Adenomatosis
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1 University of Erciyes, Faculty of
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2006 was observed a small decreasin
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for O69 strain), and clinical signs
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2 University of Veterinary Medicine
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Page 136 and 137: complete random design (CRD). The r
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Page 142 and 143: concentrations of the goat milk, in
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Page 146 and 147: cm long and 1/48 cm diameter. Middl
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Page 150 and 151: 847 Llama Anesthesia for Orthopedic
Page 152 and 153: 28 Preliminary Results of a General
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Page 156 and 157: 109 Association between Johne’s D
Page 158 and 159: 195 Changes in Plasma Beta-Carotene
Page 160 and 161: In the last year 2006, in the perio
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Page 166 and 167: The level of antibodies produced in
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Page 172 and 173: 1111 The Prevalence of Hock Lesions
Page 174 and 175: Objectives: To evaluate two differe
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Page 178 and 179: 1134 A Comparison between Two Calf
Page 180 and 181: The present work aimed to investiga
Page 182 and 183: University College Dublin, School o
Page 186 and 187: inseminator were tested in regressi
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Page 190 and 191: tissue (8,6 cm 2 ) was twice as lar
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Page 198 and 199: in each sample). On day 0, 104 cows
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Page 202 and 203: and durable spermatozoa. Within the
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Page 220 and 221: Lameness results in major economic
Page 222 and 223: methods; ANOVA (with binary classif
Page 224 and 225: Initially two radiographs were obta
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Page 230 and 231: Key words: calf, active immunity, p
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vaccinated (Group II) with Pastobov
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# Antigens Association in diarrhea
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1015 Prevalence of Eimeria Infectio
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group showed higher serum lactate c
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thymus in calves and heifers with c
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concentrations of calves with umbil
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The goal of this paper is to expose
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187 Cooling Strategies to Improve M
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inspections, carried out by specifi
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The origin of Hungarian Grey cattle
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University of Warmia and Mazury in
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POSTER ABSTRACTS 1225 The Effect of
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multiple fracture (n=1, 1.3%). The
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position. A fusiform skin incision
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Conclusions: It is the first presen
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1 Faculty of Veterinary Medicine, A
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of 101.55±4.94 mmHg. Afterwards th
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In Mexico, there is a wide variety
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Cardiovascular disorders are common
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Conclusion: The total contamination
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Material and methods: The trial inc
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detection of M. wenyonii infection.
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390 Study on Attenuation Mechanisms
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POSTER ABSTRACTS 1152 Detection of
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digestion. It works only on Apicomp
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determined. In addition the surface
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Africa and Europe, which causes mor
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Results: On D0, the animals include
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addition of mineral salt, during 12
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digestion could be improved with im
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Ltd., Blandford Forum, UK). The air
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cattle ocular squamous cell carcino
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pharmacokinetic/pharmacodynamic (PK
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Bovine Immunodeficiency Virus (BIV)
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significantly lower in groups vacci
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5.00+0.67 and 4.94+0.15 mEq/l in th
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potable water and food, avoiding st
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equal distributions at 08:00 and 16
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electrochemical properties of the a
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Selenium was discovered as an essen
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growth by measuring BW at 15-day in
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influence of different risk factors
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performances of primiparous cows ar
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anaerobes. Aerobic bacteria isolate
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P4 12 to 24 hours before calving is
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exists about Mb prevalence in dairy
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frequency of animals with BoHV anti
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esults, supported by a decrease in
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international drivers of change. Th
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The performance of two groups of no
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spasm is accompanied by kyphosis, w
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occurrence of a crisis of stiffness
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Notices 336 XXV. Jubilee World Buia
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