Oral and Poster Abstracts

calving pregnancy period were the best in the control group. The single
injection of the additional medicine increased fertility indices if compared
to cows treated intramammary only. Fertility indices were better in cows
recovered as the effect of the first treatment and worse in cows that
became chronically inflamed and required additional therapy of mastitis.
It can be concluded: acute mastitis between 10th and 100th day
postpartum elongates the period from calving to first insemination,
decreases the conception rate, and increases the insemination index,
calving pregnancy period and culling rate of dairy cows. More detrimental
are the chronic cases than acute, successfully treated cases. The single i.m.
injection of antioxidants (vit. C, vit. E, b-carotene, Se) or the lysozyme
dimer increases the efficacy of intramammary antibiotic treatment of
mastitis caused by Streptococcus spp., CNS, E. coli and even Staph.
aureus. It seems the i.m. injection of flunixin meglumine increases the
efficacy of coliform-mastitis therapy with antibiotics. However injection
of additional medicine to intramammarily treated cows sick with acute
mastitis improves their fertility indicators.
Key words: cows, mastitis, antibiotics, antioxidants, lysozyme dimer,
NSAID, fertility
129 The Contagious Staphylococcus aureus Genotype B Affects
the Presence of Other Mastitis-Relevant Bacteria in Dairy
Herds
A. Michel, A. Steiner, HU. Graber
Clinic for Ruminants, Vetsuisse Faculty, University of Bern, Berne,
Switzerland
Objectives of study: Staphylococcus aureus (S. aureus) is globally
one of the most important pathogen of subclinical mastitis and causes
large economic losses. In the present study, the effect of the contagious
S. aureus genotype B on the presence of other mastitis-associated
pathogens (coagulase-negative staphylococci (CNS), Streptococcus
spp. (non-agalactiae streptococci), and Corynebacterium spp.) was
investigated in Swiss dairy herds.
Materials and methods: Sample herds (SH): 1057 milk samples from
265 cows (10 farms) with diagnoses of S. aureus genotype B problems
were collected. As control herds (CH), 257 cows (20 farms with 1021
milk samples) were selected with at least 1 S. aureus isolate of non-B
genotype (genotypes A, C, H, E, D, R). All the samples were
bacteriologically analyzed according to standard guidelines (NMC).
Genotyping was done by ribosomal spacer-PCR. For the SH and CH,
the prevalences on cow and quarter levels of S. aureus, Streptococcus
spp., CNS and Corynebacterium spp. were compared using nonparametric
statistics. Binary logistic regression was used to select those
variables which best classified SH and CH.
Results: Considering S. aureus, the cow and quarter prevalences
clearly differed between SH and CH (p