Oral and Poster Abstracts

cattle ocular squamous cell carcinoma also react with equine sarcoid
and bovine papilloma cell, implying that they may have a common
immuno - stimulating therapy on "restricted neoplasm cell" grounds.
Whether or not cancer cells induce immunity it is probably
determined in part by whether they also cause inflammation. Hence
if a metastasizing tumor doesn't invade lymphoid organs it may be
ignored by immunity system. Conversely, on a basis of cancer cells
possess antigen embedded on cell wall, in a cow suffering from
cancer eye treated by other's cancer eye cells as antigens the
immunity system destroys its own tumor cells as cell mediated
response. Therefore the immunity system may not make any
difference between them, as it occurs in allograft, and yet when a
flogosis starts at the same time or later the treatment makes hasten
curative responses.
399 Artificial Induction of Lactation as a Remedy for Infertility
and Stray Cow Menace
S. Kumari, R. Paul, M. Sharma, S. Vaid
Veterinary Polyclinic Bhuntar at Mohal, Department of Animal
Husbandry Himachal Pradesh, Kullu, India
In India stray cow menace is increasing day by day. Farmers do not
want to keep the cows that become repeat breeders and cease giving
milk. As cow slaughter and their use for meat purpose is not an
option in India, these cows finally are left stray on the roads. These
stray animals again pose many kind of risks to the people in the form
of accidents, zoonoses etc. To reduce this problem 30 farmers were
motivated to either adopt such stray cows from the roads or not to
leave the animals stray if they had animal with such problem.Then
these animals were induced into artificial lactation so as to make
them useful. The artificial induction was carried out using a
combination of Estrogen and Progesterone injections for 9 days,
followed by an injection of Dexamethasone for five days. The
animals started lactating within 14-15 days of treatment. With the
priming of reproductive tract with these hormones, the cows that
were without any anatomical or structural abnormalities, also
conceived and delivered calves. In brief, it can be concluded that
artificial induction of lactation can be used as a remedy to reduce
stray cow menace, make infertile cows useful and to treat the repeat
breeder cows (with structurally normal reproductive tracts) that are
refractory to other treatments.
400 Associations between Lung Lesions and Grade and Estimated
Daily Live Weight Gain in Bull Beef at Slaughter
P. Williams 1 , L. Green 2
1
Schering Plough Animal Health, Livestock Business Unit, Uxbridge,
United Kingdom
2
University of Warwick, Biological Sciences, Warwick, United
Kingdom
A total of 645 bull beef from 15 units were examined at slaughter
and their lungs scored for presence and severity of consolidation and
pleurisy. Data on dead weight and carcase grade were also collected.
Pleurisy and consolidation were correlated within cattle and both
were associated with reduced estimated daily live weight gain
(EDLWG) once producer variability had been accounted for in a
hierarchical model. Consolidation had a stronger statistical
association with EDLWG and cattle with 3, 4, 5 or 6 consolidated
lobes had a significant reduction in EDLWG of 72, 113, 123 and 202
grams respectively. Cattle had a maximum of four lobes with >50%
of each lobe consolidated and those with 3 or 4 lobes with >50%
affected had a reduced EDLWG or 141 and 194 grams respectively.
Carcases with a higher grade were associated with a significantly
faster EDLWG and had fewer consolidated lobes per carcase. We
suggest that, if these associations are causal, these results highlight
the potential cost of sub-clinical lung damage on both growth and
carcase quality. These results may usefully be given to farmers to
encourage changes in management to reduce lung damage in live
cattle.
Key words: abattoir data, cattle, lung consolidation, live weight gain,
carcase quality
401 Relevance of Sulphamethoxazole/Trimethoprim Susceptibility
Ratio (19:1 or 5:1) for Bovine Mastitis Staphylococci Field
Isolates in vitro Testing
294 XXV. Jubilee World Buiatrics Congress 2008
C. Vilela 1 , S. Nunes 1 , C. Carneiro 1 , M. Dargent-Figueiredo 2 ,
L. Cavaco 1 , M. Oliveira 1 , R. Bexiga 1
1 Faculdade de Medicina Veterinaria de Lisboa, Centro de
Investigaçio Interdisciplinar em Sanidade Animal (CIISA), Lisboa,
Portugal
2 Bayer Health Care, ANIMAL HEALTH, Lisboa, Portugal
Antimicrobial susceptibility testing, used to identify resistance
patterns for different bovine mastitis pathogens, is essential to issue
recommendations for treatment at an individual or herd level.
Sulphamethoxazole/trimethoprim (SXT) has been used for mastitis
therapy in parenteral or intra-mammary form. This combination
could constitute an alternative for the treatment of methicillinresistant
S. aureus and methicillin-resistant coagulase negative
staphylococci. Current guidelines for susceptibility testing of SXT
issued by CLSI, using the disk diffusion method or the
microdillution test, recommend testing a 19:1 ratio of the 2
antimicrobials. This is based on the 19:1 human plasma
concentrations attained when a 5:1 ratio of these antimicrobials is
administered parenterally. However, when intramammary
administration is performed, a 5:1 ratio is attained in the udder. We
aimed at evaluating the minimum inhibitory concentrations of SXT
tested at the CLSI recommended 19:1 and at 5:1.Thirty S. aureus and
31 S. epidermidis field isolates from bovine intrammamary
subclinical infections were used in this study. MIC values of SXT
were determined by broth microdillution, following the CLSI (2002)
guidelines for veterinary susceptibility testing, with a modification
of the drug ratio for 5:1. Reference strain S. aureus ATCC 29213
was used. MIC was considered the lowest dilution inhibiting visible
bacterial growth. MIC values for a SXT ratio of 5:1 were compared
to the results with the ratio of 19:1.All the S. aureus isolates tested
were susceptible to SXT at a ratio of 19:1, while 9 of the 31 S.
epidermidis isolates under study were not susceptible to this
association. When testing the 5:1 ratio, 28 S. aureus and 29 S.
epidermidis isolates showed a lower MIC value. Interpretation
criteria for the 5:1 ratio are not available, therefore we cannot infer
on the susceptibility. The observed difference in MIC values
suggests that susceptibility testing results will be influenced by the
SXT ratio used in the in vitro test. Regarding intramammary
pathogens, a 19:1 ratio is adequate when parenteral administration is
concerned. However, for the same pathogens, a 5:1 ratio may likely
to reflect the drug concentration when the intramammary route is
used. Therefore, interpretation criteria for the 5:1 ratio are required,
in order to allow for susceptibility testing under these circumstances.
Key words: SXT ratio, bovine mastitis, staphylococci, MIC
402 Comparing the Effect of an Application of a Long-acting Dry
Cow Antibiotic for Drying off with the Combined Application
of a Long-acting Dry Cow Antibiotic and an Internal Teat
Seal on Udder Health of Dairy Cows State up to 100 days
Postcalving
K. Mütze 1 , W. Wolter 2 , K. Failing 3 , B. Kloppert 2 , M. Zschöck 2 ,
H. Bernhardt 1
1 Institut für Landtechnik Justus Liebig Universität Giessen, Giessen,
Germany
2 Landesbetrieb Hessiches Landeslabor, Abteilung Veterinärmedizin,
Giessen, Germany
3 Arbeitsgruppe Biomathematik und Datenverarbeitung des
Fachbereichs Veterinärmedizin der Justus-Liebig-Universität
Giessen, Giessen, Germany
The objectives of this study were to clarify whether the application
of an internal teat seal (OrbeSeal ® , Pfizer/ Karlsruhe) in
combination with a long-acting intramammary dry cow antibiotic
offers an additional protection against new intramammary
infections (IMI) during the dry period and has influence on IMI
status, clinical mastitis events and number of somatic cells up to
100 days postcalving compared with a single application of a longacting
intramammary dry cow antibiotic. A field study was carried
out from July 2006 until March 2007 with 152 cows of 12 Hessian
dairy farms. Before the final milking on the day of dry off duplicate
quarter milk samples have been taken for cytobacteriologic
analysis. The cows of each farm were parted into 2 groups by
chance. After milking all 4 quarters of the cows of group AB were
infused with long-acting dry cow antibiotic. Cows of the second
group AB+OS additionally to the long-acting antibiotic the internal