Oral and Poster Abstracts
Oral and Poster Abstracts
Oral and Poster Abstracts
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# Antigens Association in diarrhea samples %<br />
4 Rotavirus-Coronavirus-E.coli-Cryptosporidium 39.7<br />
3 Rotavirus-E.coli-Cryptosporidium 14.3<br />
2 Rotavirus-Cryptosporidium 12.6<br />
infections. The high prevalence of Cryptosporidium is noteworthy.<br />
Continued diagnosis remains valuable to guide treatment <strong>and</strong><br />
prevention practices.<br />
1008 Changes of Lymphocytes Subpopulation <strong>and</strong> Serum<br />
Immuno-chemistry Profiles of Colostrum Conferred Korean<br />
Native Calves during the First 21 th Days Postpartum<br />
YH. Jung 1 , TY. Hur 1 , SJ. Kang 1 , YJ. Do 1 , MS. Lee 1 , GH. Suh 2 ,<br />
IS. Ryu 1 , DS. Son 1 , CY. Choi 1 , JT. Kim 3<br />
1 National Institue of Animal Science, Livestock Resources<br />
Development, Cheonan, Korea, South<br />
2 Chonnam National University, College of Veterinary Medicine,<br />
Gwangju, Korea, South<br />
3 Kangwon National University, School of Veterinary Medicine,<br />
Chuncheon, Kangwon, Korea, South<br />
This study was carried out to changes of lymphocytes subpopulation<br />
<strong>and</strong> serum immuno-chemistry profiles of colostrum conferred<br />
Korean native calves during the first 21th days postpartum. A total<br />
of 65 calves were used <strong>and</strong> blood samples were collected through the<br />
cervical vein by serum separator from added vacuum tubes on 1st<br />
(pre-feeding colostrum), 2 nd , 3 rd , 7 th , 14 th <strong>and</strong> 21 th day of<br />
postpartum. The percentages values of CD2 + , CD4 + , CD8 + , CD26 + ,<br />
ACT2, MHC I, MHC II, WCI <strong>and</strong> B cells were determined using<br />
monoclonal antibodies to cell surface marker(VMRD, Pullman,<br />
USA) <strong>and</strong> FACScanto flow cytometer (Becton Dickinson<br />
Biosciences, San Jose, USA). Serum was separated from the blood<br />
immediately after the collection <strong>and</strong> stored in a -70°C deep-freezer<br />
until analysis. The proportion of MHC II cell in peripheral blood<br />
leukocyte (PBL) was significantly higher at 1st than 2nd<br />
day(p10 6 CFUs per ml), but 5 were not (10 6 CFUs per ml). Sixty-five isolates<br />
from seven calves were tested for antibiotic sensitivity by the agar<br />
disc diffusion assay. All were resistant to erythromycin, lincomycin<br />
<strong>and</strong> tetracycline <strong>and</strong> 54 to penicillin G <strong>and</strong> tylosin, but none was<br />
resistant to florfenicol. More cases will be studied <strong>and</strong> the C.<br />
perfringens isolates will be toxin typed. Further research is also<br />
needed to identify the aetiology of sudden death of bacteriologically<br />
negative calves.<br />
1011 Efficacy of Florfenicol Associated or not with Intravenous<br />
Fluid Therapy for Treatment of Experimental Salmonellosis<br />
in Neonatal Calves<br />
D. Silva, P. Silva, J. Fagliari<br />
Faculdade de Ciencias Agrarias e Veterinarias/UNESP,<br />
Departamento de Clinica e Cirurgia Veterinaria, Jaboticabal-SP,<br />
Brazil
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