Oral and Poster Abstracts

RNS causes increased lipid peroxidation and accelerated proteolysis
what is reflected in increased presence of LDH5, as hepatocytes
destruction.
Key words: cow, keton bodies, oxydative stress, ROS, RNS
70 Effect of BCS Change during the Dry Period on the
Performance of Dairy Cows
T. Van Werven, D. Speksnijder, J. Papen, M. Nielen
Faculty of Veterinary Medicine, Utrecht University, Farm Animal
Health, Utrecht, Netherlands
This abstract shows the preliminary results of an on going blind trial
on the effect of orally supplemented vitamin E during the dry period
on the performance of dairy cows. Most studies are interested in
either calving BCS or amount of BCS loss postpartum. This study
evaluates the effect of BCS change (loss or gain) during the dry
period on the postpartum performance of the cow. Five dairy farms
were selected to participate in a blind field trial to investigate the
effect of orally supplemented vit E. From each farm approximately 60
dry cows are enrolled into the study 6-8 weeks prior to calving date.
Dry cows are divided in two groups (A and B), one group is fed 135
IU and the other group 3000 IU of vit E during the dry period. Body
condition score is measured at a 5-point scale once a week during the
dry period, at calving and at the first milk test-day. Milk test-day
records are collected for fat, protein and SCC. Data on retained
placenta are collected and defined as failure to expulse the placenta
within 12 hours pp. Change of body condition score during the dry
period is calculated by regression analysis which provides cows with
an increasing or decreasing BCS during the dry period. Cows with an
unchanged BCS during the dry period are included in the group of
cows with increased BCS. Until so far milk test-day records of 94
cows are presented. At the beginning of next March all cows will be
included. From these preliminary results some interesting trends are
demonstrated. There seems to be a difference between the two
vitamin E groups (blind). Cows in group A show more often an
significant increase of BCS during the dry period than cows in groups
B, corrected for herd effects. Cows with average BCS3, corrected for increasing or decreasing slope during the dry
period. Preliminary trends indicate that cows with a BCS loss during
the dry period are less at risk for a fat/protein ratio > 1.4 than cows
that gained BCS, corrected for days in milk. These results indicate
that cows with BCS loss in the dry period might have a more efficient
energy metabolism after parturition and therefore be less at risk for
metabolic diseases like ketosis after parturition. Those cows might
experience fewer metabolic changes during the transition from late
gestation to early lactation. The possible effect of vit E on BCS needs
further research.
Key words: body condition score, dry period, dairy cows, vitamin E
71 Metabolic Syndrome in Cows: TNF-1 Concentrations before
and after Parturition in Healthy and Ill Cows
B. Fürll 1 , G. Hädriich 2 , M. Fuerll 2
1
University of Leipzig, Department of Veterinary Physiology, Leipzig,
Germany
2
University of Leipzig, Department of Internal Medicine, Leipzig,
Germany
Objectives: Today, cattle health is especially disturbed because of a
strained metabolism. Cytokines of the visceral fat depot, TNF-1 for
example, can affect the metabolism negatively. Therefore we
investigated cows with the aims: a) Are there any differences
between the TNF-1 concentrations in healthy and ill cows around
parturition? b) Can those differences be noted before parturition
already? c) Are there any TNF-1 changes specific for certain
diseases?
Material and methods: 20 healthy cows as well 103 cows with
various diseases (table) were examined 10 days before parturition (d
a.p.) and 3 d after parturition (p.p.). Blood samples were analysed
byHitachi 912, TNF-1 by an ELISA.
Results: The TNF-1 concentrations a.p. and p.p. of healthy (medians
36:37 ng/ml) and ill (140:55 ng/ml) cows differed significantly. Table:
TNF-1 (ng/ml), FFA (mmol/l) and haptoglobin (Hp, g/l) concentrations
(medians) in healthy cows and cows with different diseases p.p.
(*p=0.05)
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TNF-1 -10 35,6 90,7* 50,4 26,8 23,9 33,4 76,4 55,9*
3 37,3 117,5* 47,8 19,9 10,0 19,8 50,5 10
FFA -10 80 115 180* 150* 115 310 210* 85
3 570 870* 872* 790* 875* 875* 620 1270*
Hp -10 0.20 0.09 0.33* 0.09 0.11 0.40* 0,08 0,28
3 0.74 1.51* 1.52* 1.45* 1.22* 1.86* 1,01 1,04
The TNF-1 concentrations correlate a.p. with the back fat thickness
(p>0.05); they are at their highest (p 0.4 mmol/L, and the prevalence of herds with >15%
subclinical ketosis (cut-off of 1.4 mmol/L) was 52.6%. The within herd
prevalence of NEB and SCK ranged from 14% to 80% and 8% to 44%,
respectively. The prevalence of SARA (rumen pH 15% elevated NEFA and in 75% of
herds with >15% elevated BHB. At least one retained placenta was
reported in 85% of herds with >15% elevated NEFA and in 100% of
herds with >15% elevated BHB. Further modelling of the data will be
discussed at the meeting. The results suggest that subclinical metabolic
disease in transition cows is present in dairy herds surrounding Lima,
Peru at levels that may negatively impact milk production,
reproductive performance, and profitability. There is opportunity to