Oral and Poster Abstracts

was significant and showed that breeding in Miankale buffaloes which
are mixed breed of water buffaloes and are living in wild condition can
be done successfully.
Key words: ovsynch cider, buffaloes, Miankale peninsula, Iran
834 Isolation of Lactobacillus from the Vagina of Buffalo-cows
and its in vitro Probiotic Activity
S. Abd El-Moez1 1 , W. Ahmed 1 , J. El Jakee 2 , F. El Seedy 3
1
National Research Center, Animal Reproduction &AI, Giza, Egypt
2
Faculty of Veterinary Medicine, Microbiology, Giza, Egypt
3
Faculty of Veterinary, Beni Sueif University, Microbiology, Beni
Sueif, Egypt
Lactobacilli are among the predominant microorganisms in the vaginal
tract of some homoeothermic animals. The current investigation was
designed to throw line on Lactobacillus in the vagina of buffaloescows.
Vaginal and blood samples were collected from animals reared at
Lower Egypt for a period of 2 years. Vaginal swabs were collected
from buffalo-cows either showed normal ovarian activity or suffering
from ovarian inactivity. Swabs were collected from each animal in
either Tryptic soy broth for the routine bacteriological examination or
De Man, Rogaso, Sharpe (MRS) for isolation of Lactobacillus. In vitro
sensitivity and probiotic tests against the most predominant isolates
isolated from buffalo cows (Y. enterocolitic, C. diversus, E. coli,
Micrococcus spp., E. Faecalis, S. aureus and Bacillus spp. were carried
out). Results showed that Lactobacillus was isolated from the vagina of
normal cyclic buffalo-cows with an high incidence (90.91%) as
compared to in animals suffering from ovarian inactivity (81.82%).
Vaginal pH and the recovered isolates were recorded in relation to
ovarian activity. Ciprofloxacin and ofloxacin are the most effective
antibiotics followed by tobramycin, gentamicin and oxytetracycline.
The resistant rate of isolates to some antibiotics was recorded. In vitro
use of Lactobacillus isolated from the genital tract of normal buffalocows
as probiotic against the most predominant isolates from buffalo
cows suffering from ovarian inactivity revealed that L. acidophilus is
the most effective strain followed by L. casei rhamnosus. It could be
concluded that Lactobacillus is one of the normal bacterial floras of the
vagina of buffalo-cows; L. acidophilus and L. casei rhamnosus were
the most predominant isolates and showed high probiotic effect against
the most predominant bacteria isolated from cases suffering from
ovarian inactivity.
Key words: buffaloes, vagina-Lactobacillus, antibiotics, probiotics
835 Efficacy of Toltrazuril (Baycox Bovis) and Diclazuril
(Vecoxan) in the Control of Subclinical Coccidiosis in Buffalo
Calves
M. Diaferia 1 , F. Veronesi 1 , G. Severi 1 , D. Lulla 2 , L. Nisoli 2 ,
D. Piergili Fioretti 1
1 Faculty of Veterinary, Department of Parasitology, Perugia, Italy
2 Bayer Health Care, Milan, Italy
Coccidiosis in buffalo species is a widespread protozoa infection
caused by several species belonging to genus Eimeria and localized in
the intestine. Higher values of prevalence and diarrhoeic
manifestations are more frequently observed in young animals;
however, subclinical coccidiosis is responsible for serious economic
losses for reduction of growth rates and feed conversion in young
animals. The objective of this field efficacy study was to demonstrate
the effect of a treatment with Toltrazuril (Baycox Bovis ® ) and
Diclazuril (Vecoxan ® ) on buffalo calves naturally exposed to Eimeria
infection, in terms of lack of faecal oocyst excretion and body weight
gain. Two farms in Central Italy were selected based on previous
history of coccidiosis outbreak by E. zuernii and E. bovis and on the
presence of subclinical infections. A total of 135 buffalo calves ranged
from 4 to 15 weeks of age and from 39 to 80 kg and positive for
coccidiosis, were selected. The animals were divided in 3 distinct
groups (T, D and C) of 30 (farm A) and 15 subjects (farm B), each
based on population size of the farms. On day 0 the T group was treated
with Toltrazuril (Baycox ® Bovis) at dose of 15 mg/kg body weight; the
D group was treated with Diclazuril (Vecoxan ® ) at dose of 1 mg/kg
body weight and the C group was kept as untreated control. The
animals were examined for the presence of Eimeria species by qualiquantitative
coprological tests at 0,2,4,7,49,51,53,56 days posttreatment.
No side effects were observed in the medicated group during
the trial. On day +7, Toltrazuril showed a mean efficacy value of 98.
142 XXV. Jubilee World Buiatrics Congress 2008
85%. High values of efficacy were observed till 42 days after treatment;
on day 56 the mean value was of 65.37%. On day +7 Diclazuril showed
a mean efficacy value similar but lower than Toltrazuril (97.28% versus
98.85%); values > 50% were observed only till 23 days after the
treatment. Statistical significant difference was observed between the
animal of the T and D groups and the control group (p