Oral and Poster Abstracts

1085 Vertical Transmission of Enzootic Bovine Leucosis Infection
in a Dairy Herd with High Prevalence of the Disease in
Uruguay
R. Sienra 1 , H. Guarino 2 , G. Tort 3 , A. Hernández 3 , F. Olariaga 3 ,
J. Bianchi 3 , J. Slavica 3 , M. Alzugary 3
1
College of Veterinary Medicine, Large Animal Clinic, Montevideo,
Uruguay
2
College of Veterinary Medicine, Microbiology, Montevideo,
Uruguay
3
Assistant, Research project, Montevideo, Uruguay
Enzootic Bovine Leucosis (EBL) in Uruguay is widely distributed among
dairy cattle in the country The objective of this research was to determine
the importance of vertical transmission of the LBE in one commercial
dairy farm with a high prevalence of EBL infection, with a population of
513 Holstein cows. During the calving period a permanent control of births
were performed during the 24 hours, with the purpose of obtaining blood
samples from the newborn calves prior to the ingestion of colostrum. After
birth calves remained with their mothers for 6 to 8 hours of life, and then
they were removed to the breeding area and feeding individually with pool
colostrum and milk. A second blood sample was obtained at 24 hours postpartum.
Serum samples of mothers and calves were obtained by puncture
jugular without anticoagulant. The sera were processed through a
commercial kit EBL, indirect ELISA technique to detect the gp-51 surface
glycoprotein according with the indications of the manufacturer (IDDEX,
USA). The prevalence of infection in the herd was 60,4%, with 310
positive cows out of a total of 513. For the study of vertical transmission 99
deliveries were included, 52 from seropositive mothers and 47 from
seronegative cows. Before the intake of colostrum, all of the calves born
from uninfected cows had similar status as their mothers (47 in 47). In the
other hand, 9 of the 52 calves born from seropositive cows tested positive
to EBL before the ingestion of colostrum (17%) In the second sample, 23
of the calves whose mothers were negative were detected with EBL
antibodies (seroconvertion rate of 50%). For the group of calves born from
infected cows, at 24 hours of live, 34 tested positive (65%).The 34 calves
included 9 detected infected at birth and 25 new positive animals
(seroconversion 58%).The rate of vertical transmission observed in this
study was higher than the reported of several authors, which found
percentages between 6-8 %. The seroconversion during the first 24 hours
of life means that in practice it is very difficult to identify infections vertical
face of the presence of antibodies acquired after birth due to the
administration of colostrum and milk mixture of different cow’s
serological condition. In practical situations calves can not be controlled
before the ingestion of colostrum. Therefore EBL positive status calf
bleeding during their first days of life must be interpreted with caution.
Proyect Financed by PDT-CONICYT, Uruguay
Key words: Bovine Leukosis, vertical transmission
1086 Abscesses Disease in Small Ruminants of Batna Area
(Algeria): Preliminary Study of an Epidemiologic
Investigation
M. Alloui 1 , A. Ayachi 1 , J. Kaba 2
1 University of Batna, Veterinary Science, Batna, Algeria, Algeria
2 Agricurtural University of Warsaw, Veterinary Faculty, Department
of Epizootiology, Warsaw, Poland
The results of epidemiologic and clinical investigations, relating to the
abscess disease at the small ruminants (goat and the sheep) in the area of
Batna (Algeria) are presented. The epidemiologic study has concerned 18
extensive flocks into extensive whose the number varied between 39 and
102 heads of animals. The clinical studies were carried on 68 animals
resulting from these flocks. Clinically, the disease didn’t affect the general
state of the animal and often touched various parts of the body by one or
two abscesses per animal affected. The internal form of the disease was
not observed on 38 carcasses slaughtered in the slaughter-house of the
area. The morbidity of the disease was 1.5 p. 100 in the goat and of 5.5 p
100 in the sheep. This disease has often touched the adults whose age was
higher than one year. The frequency of the abscesses of the head (parotid
and mandibular, and retropharyngeal region) was higher than the other
parts of the body (forelimbs and posterior) (68 p. 100 against 32 p. 100) at
the two animal species. The supporting factors of the disease appearances
were: the breeding of several animal species in the same farm (sheep,
goat, bovine, and poultries), bad hygiene of the shelters, mode of course
(mountains, meadows) and starvation (food shortage).
Key words: abscess disease, epidemiology, small ruminants, Algeria
1087 The Case of BVDV-MD in a Heifer
A. Sagkan-Ozturk, A. Coskun, I. Sen
University of Selcuk, Faculty of Vet. Med., Department of Internal
Medicine, Konya, Turkey
Diseases associated with Bovine Viral Diarrhea Virus (BVDV) have been
recorded in most countries where cattle are raised and in some countries
may be the single most important virus infection of cattle. BVDV is a
pestivirus responsible for considerable economic losses in cattle industry.
There are many BVDV-infected cattle without typical clinical
manifestations of diarrhea and mucosal diseses. A large proportion of
BVDV infections are also subclinical and majority seropositive cattle.
Objective of study: The aim of this case report is to indicate BVD-MD
-infected a heifer with the severe diarrhoea and high fever along with
oral lesions.
Materials and Methods: Material of the case report was constituted
BVDV-MD -infected a Holstein- heifer, 16 months of aging. After
clinical examination, venous blood sample was taken from vena
jugularis of heifer. Taken blood samples were tested for BVDV antigen
and neutralising antibody titres. A commercial direct and indirect
ELISA kits (Institute POURQUIER) were used for the determination
of BVDV antigen and neutralising antibody titres.
Results: In routine clinical examination of heifer were observed small
lesions in palate and salivation along with high temperature. There was
diarrhea with bloodly and mucus in infected-heifer. BVDV antigen in
blood samples of heifer was detected as positive. But the presence of
BVDV antibodies in serum was seronegative.
Conclusion: Cattle that clinical symptoms such as oral lesions, high
temperature and diarrhoe with bloodly and mucus might be test for
BVDV.
This study was supported by University of Selcuk, Scientific Research
Project Coordinating
Key words: BVDV-MD, heifer, ELISA.
1088 Statistical Correlation on Serological Surveys of Bluetongue
Disease with Climate Changes in Northwest of Iran
S. Mahdavi 1 , A. Bahonar 2 , AG. Ramin 3
1 Razi Institute, Virology, Karaj, Tehran, Iran
2 University Of Tehran, Veterinary Medicine, Tehran, Iran
3 Urmia University, Animal Science Department, Urmia, Iran
In the present study, the seroprevalence of bluetongue disease (BTD) was
determined in 981 sera samples and their interrelationship to biological
vector and climatic conditions as temperature, speed of windflaw, sunny
hours, rainfall and humidity. The sera were collected from western
Azerbaijan province of Iran in 2003. The prevalence of BTD was
evaluated for serodiagnosis purposes (without considering of age, species
and sex), based on an Agar Gel Immunodiffusion test (AGID). The
infection rate was found to be 55.9%. The proportion of positive sera in
different months were significant (P