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Oral and Poster Abstracts

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amount of saponins was observed in March, in the rain season, <strong>and</strong> the<br />

lowest amount in July. These results shoed the great variation in levels of<br />

saponins during the experiment, suggesting a relation between the<br />

pluviometric precipitation <strong>and</strong>/or cycle of life in these species of<br />

Brachiaria evaluated. Acknowledgements to Coordenaçao de<br />

Aperfeiçoamento de Pessoal de Nível Superior (CAPES) <strong>and</strong> to<br />

Conselho Nacional de Desenvolvimento Científico e Tecnológico<br />

(CNPq) by financial support.<br />

Key words: brachiaria, spectrophotometry, sporidesmin,<br />

photosensitization, protodioscin<br />

521 The Effect of Dry Period versus Continuous Lactation on ßcarotene,<br />

Vitamin A <strong>and</strong> Vitamin E in the Mammary<br />

Secretion<br />

F. Schweigert 1 , A. Wesser 2 , I. Buchholz 2 , J. Raila 1<br />

1<br />

University of Potsdam, Department of Physiology <strong>and</strong><br />

Pathophysiology, Potsdam-Rehbruke, Germany<br />

2<br />

University of Leipzig, Department of Veterinary Physiology, Leipzig,<br />

Germany<br />

ß-carotene <strong>and</strong> fat-soluble vitamins A <strong>and</strong> E are accumulated during the<br />

dry period in the mammary gl<strong>and</strong> <strong>and</strong> secreted with colostrums together<br />

with the accumulated immunoglobulins. Both are of great importance for<br />

the health of the new borne calf. In this study changes in fat-soluble<br />

vitamins A, E <strong>and</strong> ß-carotene (rpHPLC) <strong>and</strong> IgG1 (immunodiffusion) in<br />

the secretion of the mammary gl<strong>and</strong> during late gestation in dry dairy<br />

cows (n=12) <strong>and</strong> those which were milked until parturition (n=5) were<br />

compared over a total period of 11 weeks starting 8 before parturition.<br />

Results showed a biphasic increase in all components during the dry<br />

period. The first approx. 30 d ap <strong>and</strong> the second closely toward parturition.<br />

In animals with continuous lactation an increase towards parturition was<br />

observed as well but maximum levels were reached earlier approx. 20 ap.<br />

Absolute levels were comparable in both groups. In both groups low<br />

levels characteristic for mature milk were reached within a few days after<br />

parturition (P

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