Oral and Poster Abstracts

731 Detection and Investigation on the within-farm Transmission
of Bovine Herpesvirus-1 in Farms Located in the Trace
District of Turkey
A. Unver 1 , N. Turan 2 , H. Yilmaz 2
1 Farmamag, Firuzkoy Yolu, Baglar Sokak, Avcilar, Istanbul, Turkey
2 University of Istanbul, Veterinary Faculty, Department of Virology,
Avcilar, Istanbul, Turkey
The aim of this study was to detect BHV-1 infection and to investigate
within-farm transmission in farms located in the Trace district of
Turkey. For this, 3 dairy farms having the animals over 20 were
selected from the Marmara region. Two visits were made to these farms
6-7 months intervals. At each visit, the nasal swabs and blood from
each animal and the milk sample from milking cows were taken. Blood
sera were analysed by ELISA (IDEXX, gB blocking) for the presence
of antibodies to BHV-1. Whereas the PCR was used to detect BHV-1
DNA in the nasal swabs and milk samples. In samples taken during the
first visit, antibodies to BHV-1 were detected in 13 of 89 animals in 3
farms. 4 of 27 (14.8%) animals, 6 of 21 (28.7%) animals and 3 of 41
(7.3%) animals were found to be seropositive in farms-1, 2 and 3,
respectively. BHV-1 DNA was detected in nasal swabs of 2 animals
and milk sample of one animal in farm-1, milk sample of 1 animal in
farm-2 and nasal swabs of 6 animals in farm-3. In samples taken during
the second visit, antibodies to BHV-1 were detected in 18 of 71 animals
in 3 farms. 6 of 24 (25%) animals, 6 of 17 (35.2%) animals and 6 of 30
(20%) animals were found to be seropositive in farms-1, 2 and 3,
respectively. BHV-1 DNA was detected in nasal swabs of 3 animals in
farm-1, nasal swabs of 2 animals and milk sample of 1 animal in farm-
2. No BHV-1 DNA was detected in the samples taken from the farm-3.
In conclusion, the BHV-1 infection was detected by ELISA and PCR in
all 3 farms. Some of the animals found to be seronegative by ELISA
were positive on PCR and these animals might be either seronegative
carriers or acutely infected. Therefore, it would be better to check the
animals in the farms by both test ELISA and PCR. Also care must be
taken to test new animals and separating the youngs from the adults.
Key words: bovine, herpesvirus-1, transmission, PCR, ELISA, Turkey
733 Outbreak of Foot-and-Mouth Disease with High Fatality Rate
in Young Lambs in Tabriz - Iran
G. Noursaadat 1 , M. Tooloei 2 , F. Rezazadeh 2
1 Large Animal Clinician, Tabriz, Iran
2 University of Tabriz, Faculty of Veterinary Medicine, Department of
Clinical Science, Tabriz, Iran
Foot-and-mouth disease (FMD) is caused by an aphthovirus (family
picornaviridae) and affects all cloven foot animals. It is endemic in
Africa, Asia, South America and parts of Europe. The disease in adult
sheep usually causes milder clinical signs than in cattle or pigs, and is
often subtle enough to go undiagnosed. In contrast, FMD in lambs has
been reported to cause high mortality during field outbreaks. From
January to April 2007, five to fifty days-old lambs of the approximately
all of the flocks pertaining to the east region of Tabriz affected with a
highly fatal disease. The morbidity and mortality rates in lambs of each
flock in this outbreak were about 10-50 and 70-80%, respectively.
Adult sheep had also been affected, but the disease in sheep was very
mild and with less frequent and extent than lambs. All affected lambs
were clinically examined. Observed clinical signs include: severe
tachycardia, high fever (up to 42˚C), tachypnea, dispnea, salivation and
drooling, development of vesicular lesions in interdigital and coronary
region of all four foot and in mouth especially over the tongue and lips
as well as lameness. Most of the affected sheep were also suffering
from lameness. At the necropsy of at least one hundred died lambs,
hyperemia and hepatization of lungs especially in the apical and ventral
lobes, swelling and enlargement of mediastinal lymph nodes,
petechiation of spleen and pericardial fats, pericarditis and pericardial
effusion, epicardial and myocardial hemorrhages with pale and
necrotic areas or the typical tiger heart appearance and hepatomegaly
were observed. Histopathologic examination revealed severe hyaline
degeneration and necrosis of myocardial fibers and marked interstitial
infiltration by mononuclear cells as well as congestion and periacinar
hepatocellular necrosis in the liver. The diagnosis of Foot-and-mouth
disease was confirmed by the evaluation of the clinical and necropsy
findings as well as histopathologic changes. Mortality rates in the
affected flocks mainly decreased through vaccination of the all affected
and unaffected lambs and sheep even one-day old lambs and also
110 XXV. Jubilee World Buiatrics Congress 2008
administration of some antibiotics such as Trimetoprim, Sulfadiazine
and Gentamycin for 72 hours.
Key words: foot-and-mouth disease, lambs, Tabriz
734 Macroscopic and Microscopic Studies on Pathological Lesions
of Deleted Ovine Livers in Kermanshah Slaughterhouse
Dr. Bahiraie 1 , Dr. Pouyanmehr 1 , M. Razmju 2
1
Razi University,Faculty of veterinary Medicine, Department of
Animal Health and Anatomy, Kermanshah, Iran
2
Faculty of Veterinary Medicine, Meat Hygiene Department, Razi
University
Objective study: The pathological survey on liver which had been
recorded from sheep carcasses in slaughterhouse of Kermanshah. The
indication of lesions which causes liver deletion during slaughtering
process.
Material and Methods: The macroscopic and microscopic
examination carried out on 160 sheep livers which were recorded in
city slaughterhouse. The samples were collected randomly during 1
year (2006). Macroscopic survey was done on each recorded liver.
Preparation for microscopic examinations carried out on macroscopic
lesions samples by use of routine histological methods. Dimension of
samples were 5x, 5x, 5x millimeters. The technique for preparation of
tissue sections by tissue processor (Histokinettte) equipment was
including: 1-Fixation (buffere formalin 10%, 2-3 days), Washing
(water 4-5 hours), dehydration (using of ascending concentrations of
ethylic alcohols70%, 80%, 90%, 100%, 100%), Clearing(Xylene),
Impregnation (paraffin 56 ˚C-68 ˚C), Blocking (paraffin Merck),
Sectioning (Microtome).The thickness of prepared sections for study
was 5. Staining was Haematoxylin & Eosin (H&E). Statistical analysis
was done by SPSS.
Results: The highest macroscopic lesion was cirrhosis and then lesions
due to hydatid cysts and Fascioulosis. In microscopic study, the highest
diagnosed lesions was Bacterial hepatitis 20 cases (12.5 %) and then
pathological lesions due to Fascioulosis 18 cases (11.35%), hepatic
cirrhosis16 cases(%10), Chronic bill ducts inflammation 16 cases(10%),
Telangiectasia16 cases (10%), Dicroselliousis 14 cases (8.75%), Chronic
hepatitis 14 cases (8.75%), Hydatid cyst 10 cases (6.25%) , Fatty Liver 8
cases (5%),Hemorrhagic Pies hepatitis 8 cases (5%), Blood congestion in
sinusoidal perihepatitis and Eosinophilic parasitical hepatitis each of
them 6 cases (3.75%), Cholangitis and fibroma 4 cases (2.5%). In this
research, centrilobular Necrosis and cholangiocarsinoma which reported
in another spices was none found (0.0%).
Conclusion: The effects of contamination to parasitical agents
identified as the most causes of deletion of sheep liver directly or
indirectly. Even thought the cirrhosis was major factor in macroscopic
judgment for deletion, but the parasitical agents are the essential factors
for deletion of lives.
Key words: sheep health, meat hygiene, pathological lesions of liver,
judgment of carcasses
736 Antibacterial Activity of Aqueous Methanol Extracts of
Zingiber Officianale, Curcuma Longa, Vernonia
Anthelmintica, Acacia Nilotica and Melia Azedarach
Z. Abbas 1 , M. Aslam 2 , M. Arshad 2 , Z. Iqba 1 , M. Ashraf 2
1
University of Agriculture, Department of Parasitology, Faisalabad-
38040, Pakistan
2
University of Agriculture, Department of Microbiology, Faisalabad,
Pakistan
Introduction: The development of drug resistance as well as
appearance of undesirable side effects of certain antibiotics has led to
the search of new antibacterial agents in particular from medicinal
plants. Therefore, the present study was conducted to assess the
antibacterial potential of aqueous methanol extract of Zingiber
officianale, Curcuma longa, Vernonia anthelmintica, Acacia nilotica
and Melia azedarach against Staphylococcus aureus, Escherichia coli
and Bacillus subtilis.
Materials and Methods: The antibacterial activity of these extracts
was tested using disc diffusion method. Filter paper discs were soaked
with 15ul of plant extracts stock solution and were allowed to dry at
room temperature for 10-15 min. A bacterial culture was uniformly
spread on the surface of Mueller Hinton agar plates using a sterile
swab. Each disc was pressed down uniformly against the surface of
agar and then examined for any inhibition zone if present. The test was