Oral and Poster Abstracts

ParaCheck, CSL/Biocor, Australia (C); and ELISA Paratuberculosis,
Institute Pourquier, France (D). ELISA A is a lipoarabinomannan
(LAM)-based indirect ELISA. The other three are based on detection of
antibodies to protoplasmic MAP antigens and (unlike ELISA A)
include sample preabsorption with a suspension of the environmental
mycobacterium M. phlei. After the ELISA testing, faecal samples were
obtained from 55 animals from the four farms, -namely the 16 animals
that had tested positive or borderline positive in two or more of the
ELISAs, a random sample of 35 animals that had tested positive or
borderline positive in only one of the ELISAs, and a random sample of
4 animals that had tested negative in all of the ELISAs- for fecal
cultutre (16 weeks incubation) and PCR analysis (IS900).
Results: Kappa concordance coefficients in pairwise comparisons of
the ELISA outcomes ranged up to 0.22 (linear kappa) and 0.25
(quadratic kappa). When the borderline positives obtained were
considered as negatives, kappa values remained low (kappa up to
0.19). Comparative scatterplots indicating the quantitative ELISA
values obtained for each animal in the sample show rather numerous
animals with high values in one test and low values in another.
Coefficients of correlation between these values in pairwise
comparisons ranged up to 0.45.
Conclusions: The results of this study confirm the limitations of both
the currently available serum ELISAs and faecal diagnostic tests
performed by us for detection of subclinical MAP infections. Some
previous studies have perhaps over-estimated the utility of the available
serum ELISAs, by assessing specificity and sensitivity in animals
whose infection status could be confirmed.
Key words: MAP, intection, ELISA, culture, PCR
1104 Impact of and Risk Factors for Mycobacterium Avium
subsp. Paratuberculosis Infection in Dairy Cattle Farms
Fj. Diéguez 1 , I. Arnaiz 2 , Mj. Vilar 1 , Ml. Sanjuán 1 , E. Yus 1
1
Instituto de Investigación y Análisis Alimentario, Facultad de
Veterinaria, Lugo, Spain
2
Laboratorio de Sanidad y Producción Animal, Xunta de Galicia,
Lugo, Spain
Objectives of study: The aims of this study were to identify risk
factors associated with Mycobacterium avium subsp
paratuberculosis (MAP) infection, and to compare mastitis indices
and reproductive performance at the herd level in farms with
different serological status.
Materials and methods: A cross-sectional study carried out in 2004 in
a total of 101 dairy farms that were randomly selected in Galicia.Each
farmer was personally interviewed in farm using a questionnaire that
included questions related to different risk factors that could be
associated with MAP infection and categorized data about udder health
and reproductive performance. Cows older than one year were bled and
samples were analysed for anti-MAP with a commercial ELISA
(IDEXX). Herds with seronegative cows, or herds with only one
seropositive cow but no cows with clinical signs consistent with
paratuberculosis during the previous year, were considered negative (-
). Herds with 2 - 4 seropositive cows, or herds with one seropositive
cow and at least one cow culled during the previous year with clinical
signs consistent with paratuberculosis, were considered positive (+).
Herds with > 5 seropositive cows, or herds classed as "positive" and in
which at least two cows had been culled during the previous year with
clinical signs consistent with paratuberculosis, were considered highly
positive (++) Multivariable ordinal logistic regression model was used
for risk factor analysis.
Results: Logistic regression seemed to indicate as major risk factors
the use of colostrum from cows with a previous positive diagnosis and
housing of replacement calves with adult cattle before age six
months. As regards disease indicators, our results indicate that the
worse the farm's MAP infection status (negative, positive or highly
positive), the higher the proportion of farms with high BTSCC; this
difference in proportions is statistically significant In the case of
clinical mastitis incidence, significant differences were only observed
between the negative farms (-) and the highly positive farms (++). As
regards reproductive performance only days open seemed to differ
when comparing the negative (-) farms with those of major level of
infection (++).
Conclusions: The results of our analysis emphasize the importance of
adequate biosecurity, with replacement rearing playing a fundamental
role to reduce the importance of the disease impact in dairies.
Key words: dairy cattle, epidemiology, MAP, infection
168 XXV. Jubilee World Buiatrics Congress 2008
1105 Realization of the Integrated Veterinary Medicine in the
Herd Health and Food Animal Production of the Cattle in
Slovakia
J. Bires, E. Pilipcinec, M. Vasil, J. Pliesovsky
University of Veterinary Medicine, Department of Cattle and Sheep
Diseases, Kosice, Slovakia
Work analyses utilization of elements of integrated veterinary
medicine from farm on table under provision of herd health
management of the cattle in the Slovakia. Production and preventive
veterinary medicine is still exploiting discipline focused on health
management and production of herd of cattle. It includes creation and
realization of health programme on farm that are outgoing from activity
of veterinary surgeon (common clinical observation of the herd,
analysis of all the relevant information relating to health, productive
situation, difficulty in herd, resp. and the way of their solution,
realization of effective prevention). Health programmes in farms of
cattle are focused on technology of feeding, husbandry, welfare,
nutrition, metabolism, control of health state of mammary gland, and
quality of milk, control of health state of locomotory system,
reproductive functions of cows, health state of calves, epidemiological
situation and prevention of transimissible infectious disease, etc. The
important part of the herd health management is situation on farm in
food safety and quality of produced animals protein and food and
enviromental safety. Veterinary surgeon during realization of health
programme on farm with cattle utilizes the elements of
multidisciplinary position, which are based on the principles of
integrated veterinary medicine (knowledge of physiology, pathologic
anatomy, nutrition, animal hygiene and protection of environment,
welfare, immunology, metabolism, regulatory mechanism, disease of
cattle, economical aspect of health, public animal health, food safety,
veterinary legislation, etc.,).
Key words: integrated veterinary medicine, herd health
1106 Study the Efficacy of Different Use of PGF 2a and GnRH
Injection Protocols in Post Partum Dairy Cows
M. Mohammadsadegh 1 , S. Lotfollahzadeh 2 , A. Bahonar 3 , T. Pazoky 1
1
Islamic Azad University, Garmsar Branch, Large Animal Clinic for
Theriogenology and Ambulatory Services, Garmsar, Iran
2
Islamic Azad University, Garmsar Branch, Large Animal Clinical
Sciences, Garmsar, Iran
3
Facutly of Veterinary Medicine, University of Tehran, Food
Hygiene, Tehran, Iran
The importance of PGF 2a and GnRH to improve reproductive efficacy on
post partum periods have been studied by many authors. Reproductive
management of lactating dairy cows using synchronization ovulation has
been reviewed by Pursely et al (1997). Experimental manipulation of
follicular grown (Thatcher et al, 2000) and new clinical uses of GnRH and
its analogues in cattle have been studied (Thatcher et al, 1993). In order to
comparing different protocols of hormonal preparation of post partum
cows 201 non pregnant Holstein cows about after 40 days of parturition
were divided as follow: (1) In 61 cows only one dose of PGF 2a was
injected and cows inseminated artificially at estrous. (2) In 31 cows two
injections of PGF 2a 14 days apart were administered and cows
inseminated artificially at estrous. (3) (Pre-Select Synch): In 31 cows two
dose of PGF 2a were injected 14 days apart and 10 days after estrous
behavior one dose of GnRH, and 7 days after it third dose of PGF 2a were
injected. The cows were artificially inseminated at estrous observation. (4)
(Select Synch): In 28 cows PGF 2a were injected 7 days after GnRH
treatment and artificially inseminated at estrous observation. Statistical
analysis (Kruskal-Wallis Test,Mann-Whitney Test, Chi- Square Test ,
Student-t Test)were used to analyze parturition to pregnancy (days open )
and conception rate in different groups. Results showed that days open
and conception rate on first service were significantly improved (p