Oral and Poster Abstracts

2 University of Veterinary Medicine, Clinic of Pigs, Kosice, Slovakia
The study presents the results of examination of 62 sheep from 3 flocks
of eastern Slovakia for the presence of Toxoplasma gondii antibodies
by complement fixation test. 13 samples were found to be positive at
the titre of 1:64, 10 were positive at 1:128 and two at 1:256 dilution.
Subsequently, DNA was isolated from blood samples of these animals.
We then used PCR analysis with specific primers in search for T. gondii
DNA. In PCR analysis, 5 of 25 (20 %) of samples tested positive.
Key words: complement fixation reaction, polymerase chain reaction,
sheep, Toxoplasma gondii
787 Relationship between Physical Exam of the Mammary Gland
and Cellularity of Milk in Santa Ines Sheep
M. Blagitz 1 , V. Gomes 1 , N. Benites 2 , P. Melville 2 , M. Azedo 1 ,
C. Batista 1 , A. Della Libera 1
1 Faculdade de Medicina Veterinaria da Universidade de Sao Paulo,
1- Department of Clinical Medicine, Sâo Paulo, Brazil
2 Faculdade de Medicina Veterinaria da Universidade de Sao Paulo,
animal science department, Sâo Paulo, Brazil
To compare milk cellularity of Santa Ines sheep and alterations in
udder physical exam, udders of 292 ewes raised in Sao Paulo were
submitted to physical exam and Tamis exam. Milk samples were
collected for bacteriological exam, CMT, microscopic SCC and
automatic SCC. Udders were ranked by results of physical exam: those
without alterations both in inspection and palpation (Rank 0); those
with alterations in inspection (Rank 1); those with alterations in
palpation (Rank 2); and those with alterations both in inspection and
palpation (Rank 3). Results showed that udders rank 3 had more
alterations in the Tamis exam than those rank 0 (p