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Oral and Poster Abstracts

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1<br />

Oregon State University, College of Veterinary Medicine, Corvallis,<br />

United States<br />

2<br />

USDA-APHIS-VS-CEAH, Fort Collins, United States<br />

3<br />

Colorado State University, Animal Population <strong>and</strong> Health Institute,<br />

Fort Collins, United States<br />

4<br />

USDA-ARS, Athens, United States<br />

Objective of study: The objective of this study was to describe<br />

antimicrobial resistance patterns of Salmonella spp. <strong>and</strong> non-type<br />

specific Escherichia coli isolated from the same animals <strong>and</strong> same<br />

environmental areas over time.<br />

Materials <strong>and</strong> Methods: Sampling occurred at a local dairy over a 12<br />

month period, at 8 week intervals. Samples were collected from animal<br />

feces (cows <strong>and</strong> calves), feed, drinking water, hospital milk, colostrum<br />

<strong>and</strong> alleyway flush water.<br />

Results: Salmonella serotypes recovered from environmental <strong>and</strong> fecal<br />

samples collected from the same groups of animals differed.<br />

Salmonella serotypes were different between calves <strong>and</strong> cows. In<br />

addition, antimicrobial resistance patterns of both Salmonella <strong>and</strong> E.<br />

coli were different.<br />

Conclusions: Based on our data, we conclude that under field<br />

conditions Salmonella <strong>and</strong> E. coli may not exchange genetic material as<br />

readily as previously suggested. These results may be partially due to<br />

effective biosecurity procedures on this particular dairy, avoiding<br />

cross-contamination between cow <strong>and</strong> calf-areas.<br />

Key words: antimicrobial resistance, dairy cattle<br />

278 Subconjunctival (SC) Injection of Penicillin-Streptomycin in<br />

Keratoconjunctivitis of Calves <strong>and</strong> Adult Cattle - an Efficient,<br />

Economic Approach<br />

E. Bani Hassan 1 , T. Taghipour Bazargani 1 , R. Rahchamani 1 ,<br />

H. Sharifi 2 , A. Hosseinzadeh 3 , I. Ashrafi Tamay 1 , Sh. Moaddab 1 ,<br />

E. Torki 1<br />

1<br />

Faculty of Veterinary Medicine, University of Tehran, Department<br />

of Clinical Science, Tehran, Iran<br />

2<br />

Faculty of Veterinary Medicine, University of Tehran, Department<br />

of Food Hygiene, Tehran, Iran<br />

3<br />

Urmia University, Department of Veterinary Microbiology, Urmia,<br />

Iran<br />

Objectives: To determine the efficacy of IC-therapy <strong>and</strong> comparing<br />

this method to other common therapies.<br />

Materials <strong>and</strong> methods: The study was carried out as a pilot in two<br />

dairy farms <strong>and</strong> after approval of the efficacy of subconjunctival<br />

therapy we used this method to as the routine treatment for<br />

conjunctivitis <strong>and</strong> keratoconjunctivitis. At the first stage we showed<br />

that the single SC injection of penicillin-streptomycin, showed<br />

completely superior results compared to other methods including<br />

systemic antibiotic therapies with oxitetracycline, oxitetracycline plus<br />

tylosine, florefenicole, <strong>and</strong> even systemic injection of penicillinstreptomycin<br />

(p=0.0001).In the next step we choose the method as the<br />

usual treatment for the disease in both calves <strong>and</strong> adult cattle. This<br />

resulted in deletion of the problem of blindness <strong>and</strong> growth retardation<br />

due to keratoconjunctivitis (P

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