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distributed over 13 different fattening farms. In the farms of origin, the calves of group EV (early vaccination) were either vaccinated twice at 1 month interval with a trivalent vaccine (bovine respiratory syncytial virus (BRSV), bovine parainfluenza type 1 (PI3), Mannheimia haemolytica), or twice at 1 month interval with a bivalent vaccine (BRSV, bovine viral diarrhoea virus (BVDV)). The vaccination of group EV was completed 15 days before transport to the trading center. A second group LV (late vaccination), of calves, that arrived at the trading centers at the same time as the EV group, was left unvaccinated until the arrival at the trading center. The animals of the group LV were vaccinated with the bivalent vaccine at the trading center (first dose) and the fattening farm (second dose, 1 month later). The two groups were raised under the same conditions in each fattening unit. The following parameters were recorded: morbidity and mortality due to respiratory disease, number of treatments due to respiratory disease during the first two months at the fattening unit. The observations were performed by persons that were not aware of the grouping. Results: The results showed a significant decrease in morbidity (5% versus 12.7%) and number of individual treatments (5.4% versus 18%) for the animals that received their vaccination at the breeding farm (group EV) as compared to the calves that were vaccinated at the trading center and the fattening unit (group LV). Conclusions: Timing of vaccination has a significant effect on the prevention of respiratory disease under the conditions described above. 672 The Use of ELISA for the Diagnosis of Goat Tuberculosis in Brazil C. Marassi 1 , C. Almeida 2 , S. Pinheiro 2 S. Vasconcellos 2 , W. Lilenbaum 1 1 Universidade Federal Fluminense, Microbiology, Niterói - RJ - Brasil, Brazil 2 Universidade de Sao Paulo, Preventive Medicine and Public Health, Sao Paulo, Brazil Bovine tuberculosis remains an endemic infection in several countries, despite several efforts to control the disease. In the past, it was believed that goats were naturally resistant to tuberculosis; however it is now well established that M. bovis and M. caprae could be responsible for this disease in goats. After one clinical case that was presented to the veterinary service of the University of Sao Paulo, Brazil, a complete screening by intradermal tuberculin test was performed in the herd. The goat herd was composed by 500 animals and 83 (16.6%) of them showed to be reactive to the comparative double cervical intradermal test. Four months after the test, all the reactive animals were slaughtered and 33 of them were randomly chosen for histopathological, serological and bacteriological procedures. Histopathology (Ziehl-Neelsen and Hematoxyllin-eosin) evidenced at least one characteristic lesion of tuberculosis in each animal, with typical granulommas where BAAR could be observed. Bacteriology (inoculation in Stonebrink-Lesslie media) was positive for M. bovis in 25 samples (75.8%), therefore confirming the etiology of the outbreak. Sera of these 33 animals were tested in an ELISA using the recombinant M. bovis protein MPB70 as capture antigens. From those, 31 were reactive to the test, with high ODs results, considering a cut-off point established by ROC curve analyzing results of M. bovis culture from tuberculous cattle (cut-off = 0.06; mean= 0.55; range: 0.157-1.357). These preliminary results suggested that MPB70-ELISA can be considered as a reliable tool to diagnose tuberculosis in goat herds, since this assay was capable to demonstrate the disease in 93.9% of the animals here examined. Key words: tuberculosis, goats, ELISA, diagnosis, M. bovis 673 Surveillance of Bluetongue Virus in Domestic Ruminants in Territory of the Slovakia- Draft of Research Program J. Bires, A. Kocisova, M. Mojzis, J. Pliesovsky, V. Petrovic University of Veterinary Medicine, Department of Cattle and Sheep Diseases, Kosice, Slovakia The research project is focused on acquirement of primary information about occurrence of health and economically serious orbivirus transmissive disease - bluetongue in cattle, sheep and goats in Slovakia. In content of project there will be the research of occurrence of vector Culicoides spp. and their position towards transmission of catarrhal fever on animals (entomological study), monitoring of potential occurrence of disease in cattle, sheep and goats (clinical and laboratory examinations) and verification and introduction virological diagnosis 94 XXV. Jubilee World Buiatrics Congress 2008 of aetiological factor of bluetongue. Diagnostic procedures of bluetongue on the level of orbivirus, vector and animals are in accordance with Manual of diagnostic tests and vaccines for terrestrial animals (OIE) and legislative issues of European Commission. The intensity of surveillance bluetongue will be realised on selected areas of Slovakia during whole year on all the levels of observation depending on climatic conditions and persistency of vectors in nature. Solution of project expects collaboration of experts from veterinary university, state veterinary institute, state veterinary administration, veterinary institute, veterinary surgeon and farmers. Key words: bluetongue, surveillance, domestic ruminants 674 Prevalence of BVDV Infection in Large and Small Cattle Herds in Southern Backa and SREM District in Serbia T. Petrovic 1 , S. Lazic 1 , D. Bugarski 2 , B. Djuricic 3 1 Scientific Veterinary Institute Novi Sad, Virology, Novi Sad, Serbia 2 Scientific Veterinary Institute Novi Sad, Department of Epizootiology and Health Protection of Ruminants, Novi Sad, Serbia 3 Faculty of Veterinary Medicine Belgrade, Department for Infectious Diseases- Epidemiology, Novi Sad, Serbia In order to determine the prevalence of BVDV infection in small and large cattle herds in Southern Backa (S. Backa) and Srem district, the examination was carried out on presence of VN antibodies in blood sera in breeding cattle that were older than 6 months during 2004. The examined 3457 samples were from small cattle herds in S. Ba_ka (9 communities) and 4120 samples in Srem district (8 communities). The examination included animals from all the settlements (156) in this area. The examined 2721 samples were from 18 large cattle herds from S. Backa, and 298 samples were from 4 large cattle herds from Srem district. The examination included animals from all large herds (22) in this area. Virus neutralization test (VN) was performed according to standard procedure with small modifications. In the test NADL strain of BVD virus and cell culture MDBK were used. The sera were examined in double solution from 1:2 to 1:512.The presence of VN antibodies was discovered in 1833 (24.19%) samples from small cattle herds. Out of this number in S. Backa district there were 1082 (31.30%), and in Srem district there were 751 (18.23%) seropositive animals. The largest number of seropositive animals in S. Backa district was determined in Titel municipality (68.07%) and the smallest in Backi Petrovac municipality (6.63%). In Srem district the highest percent of seropositive animals was found in Beocin municipality (34.16%) and the lowest in Irig municipality (7.62%). The presence of VN antibodies was discovered in 1667 (55.22%) samples from large cattle herds. Out of this number in S. Backa district large herds there were 1500 (55.13%), and in Srem district large herds there were 167 (56.04%) seropositive animals. The prevalence of seropositive animals in big cattle herds from S. Backa district ranged from 0% (5 large herds) to 96.61%. High prevalence (>70%) was determined in 7 out of 18 herds. The prevalence of seropositive animals in large cattle herds from Srem district ranged from 0% (1 big herd) to 96.96%. High prevalence (>70%) was determined in 2 out of 4 herds.The obtained results point out that BVD infection is spread on the examined area. At the same time, prevalence is not equal, what means that there are areas where it is not present, or is only sporadically present. Key words: BVDV, VN test, prevalence 675 Antimicrobial Resistance in Escherichia coli Isolated from Calves in Slovakia V. Kmet 1 , D. Bujnakova 1 , D. Demankova 1 , S. Novak 1 , R. Bobcek 2 , T. Szakal 2 , M. Huska 2 1 Slovak Academy of Sciences, Institute of Animal Physiology, Kosice, Slovakia 2 University of Veterinary Medicine, Department of Cattle and Sheep Diseases, Kosice, Slovakia A total of 159 faecal Escherichia coli isolates obtained from calves from seven regions in Slovakia during one year were examined for their quantitative susceptibility. MIC values to 20 antimicrobial agents were determined by a colorimetric microdilution test. The underlying resistance mechanism were tentatively identified from antibiogram profiles using interpretative reading method. The most frequently found resistance was to tetracycline (76 %), streptomycin (77 %) and ampicillin (78 %). Among tested E . coli isolates also high resitance rates to neomycin (51 %), chloramphenicol (30 %), cotrimoxazole (28
%) and spectinomycine (25%) could be recorded. Resistance to ampicillin+sulbactam (8,1 %), ceftiofur (4,4%), apramycin (0.6 %), gentamicin (7 %) and florfenicol (10 %) was lower. All of the tested E. coli isolates were sensitive to cefquinome and colistin. Resistance to ciprofloxacin (17 %) and enrofloxacin (16,9%) was intermediate. However, overall quinolone resistance detected by means of nalidix acid was as high as 29 %. The high level fluoroquinolone resistance (CIP: 4mg/L and ENR: 16 mg/L) (18%) as well as penicillinases ((TEM1,2/SHV1)-low (51%) and (TEM1,2/SHV1)-high (25%) was detected in E. coli isolates. This is the first report of a quantitative antibiotic susceptibility testing and phenotype resistance mechanisms determination in E. coli isolated from calves in Slovakia. Key words: E. coli, minimal inhibitory concentration, interpretative reading, quinolone resistance, calves 676 Deciphering the Value of Chlamydia-Serology in Cattle J. Boettcher, A. Vossen, A. Gangl, N. Meier, G. Wittkowski Bavarian Animal Health Service, Poing, Germany Chlamydiae are often suspected in reproductive and respiratory diseases of cattle. Because of frequently used ELISA-testing, we tried to assess its value. Based on 21683 sera a seroprevalence (SerP%) of 37.0%±0.3% was determined for Bavaria (Idexx-ELISA). Positive sera were detected in 92.8%±0.9% of the farms (n=765). Average within-herd prevalence was 37.0%±1.4%. Age-related effects (n=3522): SerP% was highest in calves less than 9 month of age (mona) (48%±6%) and lowest between 21-24 mona (41%±2%)(n.s.). On 5 clinically unaffected farms swabs were analysed for Chlamydia by PCR (357 animals). Detection rates for vaginal, nasal and conjunctival swabs were 2.5%±0.8%, 2.0%+0.7% and 1.1%±0.6%, respectively. Detection rate was 22% for calves 12 months (p=0.014). SerP% in ELISA were 36% and 10% for Idexx and Pourquier, respectively. While SerP% (Idexx) increased from 22% in calves
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130. évfolyam Különszám II. Vol
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The World Buiatrics Congress (WBC)
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lood and liver tissue. Absolute vit
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RNS causes increased lipid peroxida
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An experiment was conducted to stud
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2 Hungarian Institute of Agricultur
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and plasma HCO 3 - increased from H
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Materials and Methods: Serum sample
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vitamin A stores in liver, the hypo
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andomly assigned to one of five tre
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observed in group II of the animals
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The aim of the monitoring was to co
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health group (HG) categories for an
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lactating cows is sparse. Lotan and
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491 Effect of Acarbose on Milk Yiel
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499 Secondary Hepatogenous Photosen
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they all died in a two to three day
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and HCO 3 level. The concentration
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amount of saponins was observed in
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Water was offered ad-libitum. Feed
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Se, I and Co) more than 500 blood s
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541 Variability of Blood Profile an
Page 46 and 47: evaluated, highly significantly gre
Page 48 and 49: we measured sensitivity of the enti
Page 50 and 51: were 0.17; 0.13 °C (Max.: 0.73). D
Page 52 and 53: calving pregnancy period were the b
Page 54 and 55: 2 University of Nottingham, Departm
Page 56 and 57: animals) was treated with 5 mg enro
Page 58 and 59: In dairying, good management practi
Page 60 and 61: Somatic cell count (SCC) is one of
Page 62 and 63: This investigation was run in some
Page 64 and 65: eproductive data showed that cows f
Page 66 and 67: (3.6%) and others (Bacillus spp., N
Page 68 and 69: 602 Coagulase Gene Polymorphism of
Page 70 and 71: 2 Institute of Veterinary Bacteriol
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Page 74 and 75: 4 Universidade de Sao Paulo, Clinic
Page 76 and 77: incubated for one hour at 37 oC. Af
Page 78 and 79: In Belgium, an increasing amount of
Page 80 and 81: Conclusion: It can be concluded tha
Page 82 and 83: calves recovered after 4 to 10 days
Page 84 and 85: disease. Thus losses were estimated
Page 86 and 87: 635 Prevalence of the Pulmonary Les
Page 88 and 89: Conclusions The sanitation program
Page 90 and 91: Italy. After the sample examination
Page 92 and 93: Results: The cows sera were collect
Page 94 and 95: (Mb) as co-infecting agent is becom
Page 98 and 99: main infection sources to the urban
Page 100 and 101: 686 Characterisation of Cattle Orig
Page 102 and 103: using EPFC. The intradermal tubercu
Page 104 and 105: three cattle farms each. Leptospiro
Page 106 and 107: (cELISA) only to definitively confi
Page 108 and 109: 1 Islamic Azad University of Shahre
Page 110 and 111: Financial support: CNPq, CAPES, FIN
Page 112 and 113: 731 Detection and Investigation on
Page 114 and 115: necropsied, tissue samples were tak
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Page 120 and 121: decrease in animal aggregation in p
Page 122 and 123: 747 Effect of Dietary Supplementati
Page 124 and 125: 755 Study of Pulmonary Adenomatosis
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Page 128 and 129: 2006 was observed a small decreasin
Page 130 and 131: for O69 strain), and clinical signs
Page 132 and 133: 2 University of Veterinary Medicine
Page 134 and 135: 794 Serum Haptoglobin and Fibrinoge
Page 136 and 137: complete random design (CRD). The r
Page 138 and 139: - Ca, Mg, K, and Ca:P ratio were lo
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Page 142 and 143: concentrations of the goat milk, in
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cm long and 1/48 cm diameter. Middl
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circular external skeletal fixator
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847 Llama Anesthesia for Orthopedic
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28 Preliminary Results of a General
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Israeli commercial dairy herds cons
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109 Association between Johne’s D
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195 Changes in Plasma Beta-Carotene
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In the last year 2006, in the perio
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serious economic concern in the fat
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was a cumulative incidence rate of
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The level of antibodies produced in
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1096 Determination of Risk Indicato
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ParaCheck, CSL/Biocor, Australia (C
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1111 The Prevalence of Hock Lesions
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Objectives: To evaluate two differe
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1126 Relationships between Paratube
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1134 A Comparison between Two Calf
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The present work aimed to investiga
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University College Dublin, School o
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214 Cystic Ovarian Diseases in Dair
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inseminator were tested in regressi
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University of Nottingham, Animal He
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tissue (8,6 cm 2 ) was twice as lar
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libido in unmotivated males. Simila
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groups according to their diameter
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improve embryonic development are m
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in each sample). On day 0, 104 cows
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1 Warsaw University of Life Science
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and durable spermatozoa. Within the
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motility and CM integrity (p=0.869
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the median number of attempted moun
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909 The effect of “GnRH” and
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3 COOPEVOLIA, Centre Zootechnique,
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923 Production of Bovine Blackleg V
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Institute of Physiology and Sanocre
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2 University College Dublin, Animal
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(I.M.) 1 mg of estradiol cipionate
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Lameness results in major economic
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methods; ANOVA (with binary classif
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Initially two radiographs were obta
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number of positive faecal samples d
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Pfizer Animal Health, Veterinary Me
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Key words: calf, active immunity, p
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1 University of Camerino, Veterinar
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vaccinated (Group II) with Pastobov
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# Antigens Association in diarrhea
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1015 Prevalence of Eimeria Infectio
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group showed higher serum lactate c
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thymus in calves and heifers with c
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concentrations of calves with umbil
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The goal of this paper is to expose
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187 Cooling Strategies to Improve M
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inspections, carried out by specifi
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The origin of Hungarian Grey cattle
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University of Warmia and Mazury in
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POSTER ABSTRACTS 1225 The Effect of
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multiple fracture (n=1, 1.3%). The
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position. A fusiform skin incision
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Conclusions: It is the first presen
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1 Faculty of Veterinary Medicine, A
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of 101.55±4.94 mmHg. Afterwards th
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In Mexico, there is a wide variety
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Cardiovascular disorders are common
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Conclusion: The total contamination
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Material and methods: The trial inc
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detection of M. wenyonii infection.
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390 Study on Attenuation Mechanisms
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POSTER ABSTRACTS 1152 Detection of
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digestion. It works only on Apicomp
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determined. In addition the surface
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Africa and Europe, which causes mor
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Results: On D0, the animals include
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addition of mineral salt, during 12
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digestion could be improved with im
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Ltd., Blandford Forum, UK). The air
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cattle ocular squamous cell carcino
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pharmacokinetic/pharmacodynamic (PK
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Bovine Immunodeficiency Virus (BIV)
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significantly lower in groups vacci
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5.00+0.67 and 4.94+0.15 mEq/l in th
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potable water and food, avoiding st
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equal distributions at 08:00 and 16
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electrochemical properties of the a
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Selenium was discovered as an essen
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growth by measuring BW at 15-day in
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influence of different risk factors
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performances of primiparous cows ar
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anaerobes. Aerobic bacteria isolate
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P4 12 to 24 hours before calving is
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exists about Mb prevalence in dairy
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frequency of animals with BoHV anti
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esults, supported by a decrease in
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international drivers of change. Th
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The performance of two groups of no
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spasm is accompanied by kyphosis, w
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occurrence of a crisis of stiffness
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Notices 336 XXV. Jubilee World Buia
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