Oral and Poster Abstracts

post translational modifications. We found no putative coil-coiled
structure in ovine TLR7 protein by 14, 21 and 28 windows analysis.
The putative structural analysis was performed by TMHMM as well as
PFAM servers, wherein we found that the sheep TLR7 ectodomain
starts at amino acid position (AA) 27 (LRR N terminus 27 to 60 AA)
and terminates at 831 (LRR C terminus 808 to 831 AA). PFAM
showed 5 LRRs in ectodomain lied between 222-244, 311-334, 648-
670, 697-718 and 745 to 767 AA with resemblance of super-repeat
motifs. We predict that these regions are the most probable functional
regions involved in PAMPs recognition. The other variable or
conservative forms of LRRs of ovine TLR7 are not yet predicted,
however, we suppose their presence in the ectodomain. The
transmembrane domain spanned between 838 and 866 according to
TMHMM probability prediction and the TIR domain spanned between
890 and 1029. This study makes a benchmarking for further ovine
TLR7 gene analysis like SNPs detection, protein structural and
functional analysis.
Key words: TLR7, sheep, LRR, PAMP, toll like receptor
798 Drug Interaction of Succinylcholine and Ivermectin in Sheep
A. Raayat Jahromi, N. Vesal, S. Nazifi
School of Veterinary Medicine, Shiraz Univrsity, Shiraz, Iran,
Clinical Sciences, Shiraz, Iran
Drug interactions subject is one of the most important aspects of
therapy when two or more drugs are administered concurrently. In a
former study, an unusual reaction to succinylcholine was observed
following ivermectin administration. The aim of this study was to
evaluate possible interaction between these drugs. Ten native male
adult sheep, about one year old, were randomly assigned to control and
treatment groups (n=5). In order to study the possible effect of
ivermectin on plasma cholinesterase activity, measurement of serum
enzyme activity was performed by manual and automated methods
before ivermectin (0.02 mg/kg, SC) and normal saline (as placebo, SC,
in control group) injection at following intervals: 72 hours before
anesthesia, immediately before anesthesia, 1, 3, 5 and 15 days after
anesthesia. Following premedication with xylazine, anesthesia was
induced with ketamine and maintained with halothane in oxygen. After
stable anesthesia, succinylcholine (0.1 mg/kg) was administered
intravenously. Temperature and cardiopulmonary parameters (heart
rate, respiratory rate and end-tidal PCO 2) were recorded during
anesthesia at 5 minutes interval. Time from succinylcholine injection to
apnea, depression of twitch reflex, recovery times (return of
swallowing reflex, spontaneous breathing and voluntary
movements)and times to sternal and standing position were measured
in both groups. Data analysis was done by one-way ANOVA and
independent t-test and values of P0.05) although sheep fed with 0.1
% enzyme showed a little higher plasma glucose concentration than sheep
fed with 0.05 % enzyme (p0.05).
Result: This research showed that enzyme usage in two levels in
comparison with no enzyme application in diet has no effect on the
plasma glucose level (P>0.05) although sheep fed with 0.1 % enzyme
showed a little higher plasma glucose concentrations than sheep fed
with 0.05 % enzyme (p0.05) and also the blood urea nitrogen
concentration are not different (p>0.05) among treatments can be
connected with that diets were isoenergetic and have similar protein
percentages. Hristov et al. (1998) found that enzyme in diet of animal
has no effect on the plasma glucose and blood urea nitrogen
concentrations.
Key words: NATUZYME, sheep nutrition, enzyme complex, plasma
glucose, blood urea nitrogen (BUN)
801 Effect of Feeding NATUZYM (a Multi Enzyme Complex)
on Ruminal pH Level and Ammonia Nitrogen Concentration
in Sheep
H. Baghershah, K. Rezayazdi, M. Dehghan-banadaky, A. Zali,
M. Khansefid
University of Tehran, Animal Science, Karaj, Iran
In this research Twenty-four Varamini male sheep (An Iranian
vernacular breed) with initial birth weight of 22±1.5Kg were used to
evaluate the effects of feeding an enzyme complex on the ruminal pH
and ammonia nitrogen concentration in sheep. . The sheep were
divided into three groups and fed with three diets: a diet without the
enzyme (control diet) and diets contained 0.05% and 0.1% enzyme. All
diets include 1:1 forage to concentrate ratio. . Enzyme added to
concentrate and then mixed with forage in order to make a uniform diet.
All Sheep were fed at adlibitum level. Diets were isoenergetic and
contained similar protein percentages. The design of this research was
Sheep, Goat and Buffaloes 133