Oral and Poster Abstracts

Material and methods: The trial included 18 Czech-Pied bulls fed
a diet with a high proportion of concentrated feed and 18 Czech-Pied
bulls fed a diet based on maize silage, used as a control group. The
trial was launched after the weaning of calves. During the fattening
period, live weight and average daily weight gain were monitored.
The bulls were slaughtered at the live weight 550 - 600 kg, mean age
at the slaughter was 473 days for the high-grain diet group and 474
days for the control group. The carcasses were classified to SEUROP
quality grades, and net weight gain and carcass yield were
calculated. Samples of m. longissimus pars thoracis were withdrawn
from five bulls in each group to examine selected meat quality
parameters.
Results: In the period from weaning to slaughter, the high-grain diet
bulls and the control bulls achieved average daily weight gain of
1.29 kg and 1.21 kg, respectively. Differences between the groups
were not significant. The high-grain diet group showed a higher
average carcass weight and higher net weight gain, differences
between the groups were not significant, too. As to meat quality
parameters under study, a significant difference was found only in
meat lightness (L*), with the mean value in the high-grain diet group
being significantly (P < 0.01) lower than in the control group. Other
meat quality parameters did not show any significant differences
between the groups.
Conclusions: In this study, the high-grain diet gave similar
performance as the maize silage based diet in fattening bulls. The highgrain
diet group and control group showed comparable average daily
weight gain and selected carcass and meat quality parameters.
Key words: concentrated feed, weight gain, carcass weight, m.
longissimus
1217 Quantification of Salmonella in French Cattle Fecal Samples
at Slaughter House by MPN Real Time PCR
A. Dayhum, A. Fares, M. Sanaa, O. Cerf, Y. Millemann
Ecole Vétérinaire d'Alfort, Unité Epidémiologie et Analyse de Risque,
Maisons-Alfort, France
As a part of our effort in quantitative risk analysis of food-borne
diseases, the objective of this study was to develop rapid and reliable
protocols for detection and quantification of Salmonella in fecal
samples at slaughter house. First, for the detection of Salmonella in
artificially and naturally contaminated fecal samples, SYBR Green I
real-time PCR assay was used, where quantification of Salmonella
was achieved by combining this assay with most-probable-number
(MPN) method (MPN-real-time PCR). To develop or test this
protocol for detecting and enumerating of Salmonella in artificially
contaminated fecal samples, a Salmonella enterica serotype
Typhimurium DT104 strain was inoculated into fecal samples at
different levels of contamination. Data on artificially contaminated
fecal samples indicated that both detection and quantification
protocols were able to detect and enumerate as few as 1 CFU/mL of
fecal after 8-h of a single non-selective pre-enrichment step in
buffered peptone water. All MPN estimates corresponded well to
inoculum levels. The protocol was then applied to naturally
contaminated fecal samples. A total of 296 fecal and 26
environmental samples were aseptically collected from
slaughterhouse located in Meaux, France weekly during February to
March 2006 (an average of 40 samples per visit). Salmonella was
positive from 9.12% (27/296), 34.62% (9/26) fecal and
environmental samples respectively, with estimated MPN values or
counts of Salmonella ranging from 1400 MPN/g), while all the other 21 animals were less than 80
MPN/g (from them 13 animals 0.05) Also the most important cause
of microbial contamination and high microbial load of
hamburgers was belong to contamination of meat and carcasses in
slaughterhouses due to poor and unhygienic handling
and undesirable sanitary situation during production and distribution
of meat.
Key words: hamburger, microbial quality, Iran
1219 Comparative Survey on Hygienic Quality (Coliform ,
Escherichia coli and Staphylococcus aureus) of Industrial
Butters with Using Standard Methods and Impedance -
Splitting Method
A. Fazlara 1 , A. Khataminia 2
1
Shahid Chamran University, Food Hygiene and Quality Control
Department, Ahvaz, Iran
2
Veterinary Organization, Ministry of Jihad-e-Keshavarzi, Ahvaz,
Iran
This study was conducted to compare impedance - splitting method
and conventional standard (reference) methods to evaluate microbial
quality of produced pasteurized butters in Ahvaz area in Khuzestan
province of Iran. In this study, 90 sample of pasteurized butter from
different batches were collected during warm and cold seasons (from
October 2006 to September 2007) from market places in Ahvaz city
and tested for coliforms, Escherichia coli and Staphylococcus aureus
by both methods. The standard methods were based on references
and standard institute and industrial investigation of Iran’s
recommendation and the impedance method was done by measuring
the variation of electrical resistance of broth media (M-value) and
electrodes (E-value) for detection of coliform, Escherichia coli and
Staphylococcus aureus respectively. According to the results, there
was no contamination to coliforms and Escherichia coli in
pasteurized butters and the both methods were totally correlated and
the specifity of impedance method was 100 percent. Also 5 (5.5%)
and 3 (3.3%) of all samples were contaminated to Staphylococcus
aureus with standard reference method and impedance – splitting
method respectively , and the sensitivity of impedance method
was calculated equal to 60 percent. The difference of methods was
not statistically significant (P>0.05). Comparing the results in warm