Oral and Poster Abstracts

concentrations of calves with umbilical hernia were determined by
means of sodium dodecyl sulphate-polyacrylamide gel
electrophoresis and were compared with time 0.
Results: Five plasma and peritoneal fluid proteins (ceruloplasmin,
transferin, albumin, haptoglobin, and acid glycoprotein) were
increased in calves with umbilical hernia after surgery, compared
with baseline values. Peritoneal fluid findings: ceruloplasmin
(201.67 to 530.33%) and transferin (117.28 to 251.85%) showed the
highest percentage increase in concentration from day 1 to 15. The
highest percentage increase in concentration of haptoglobin (48.67
to 400%) and albumin (66,41 to 131,83%) were observed from day 1
to 7 and were lower than the other two previously mentioned. Acid
glycoprotein showed the highest percentage increase in
concentration on day 3 (355.4%). Plasma protein concentrations:
Haptoglobin was the protein with the highest percentage increase in
concentration (88.39 to 366.6%) from day 1 to 5. These values were
higher than those found in the peritoneal fluid during the same
moments.
Conclusions: Results indicate that acute phase proteins increase more
rapidly after the onset of inflammation in the peritoneal fluid than in
plasma, except for haptoglobin. Because of this, it may be possible to
use measurements of these proteins to monitor the progression of the
inflammatory response in the abdominal cavity after surgery.
Supported by grants from FAPESP (07/51389-4). Authors thank Mr.
Paulo César da Silva for his invaluable help in the acute phase protein
quantitations.
Key words: umbilical hernia, calf, acute phase proteins
1037 In vitro Evaluation of Curd Formation after Reconstitution
of Neonatal Calf Oral Rehydration Therapy Solutions in
Whole Cow’s Milk
G. Nappert 1 , P. Domps 2
1
Lachute Veterinary Hospital, Veterinary Consultancy and Research,
Lachute, Canada
2
CEVA santé Animale, Livestock Business Unit, Libourne, France
Objective: To compare the in vitro effects on whole cow’s milk curd
formation of 40 neonatal calf oral rehydration therapy (ORT) solutions
commercially available in Europe and in Canada.
Materials and Methods: Homogenized raw milk from a
commercial dairy farm was used and kept at 30 °C on a magnetic
stirring module with an electronic contact thermometer. The ORT
solutions were reconstituted in triplicate in 50 mL of whole cow’s
milk according to the manufacturer’s directions. The pH and the
osmolarity of each test solution were measured after
reconstitution. In vitro milk clotting was performed in triplicate,
using rennin as the clotting agent. The rennin, extracted from calf
abomasum, was diluted with double-distilled water to a
concentration of 12.5 g/L with a proximal activity of 50 U/mg
protein. The rennin solution (0,1 mL) was added to each test solution
(50 mL). Measurement of clotting time was determined by a sudden
variation in the conductivity of the solution within a 10-minute
period. Objective assessment of the weight of the coagulum was
measured with a desiccator scale.
Results: After reconstitution, only four products had osmolarities
between 400-600 mmol/L. ORT solutions containing large amounts of
bicarbonate, and/or citrate (>40 mmol/L), presented a significant
alkaline pH after reconstitution and had negative effects on curd
formation. Glucose-rich ORT solutions also had significant negative
effects on milk clotting time. Isotonic oral electrolyte solutions that
contained mainly acetate and propionate did not interfere with milk
clotting time and curd formation.
Conclusions: In accordance with previous studies, the feeding of
diarrhoeic calves with whole cow’s milk is recommended in
combination with isotonic metabolisable base ORT solutions. It has
already been documented that suckling bicarbonate/citrate-rich ORT
solutions result in sustained abomasal alkalisation. For diarrheic
calves, this may reduce the digestibility of milk.
Key words: calves, diarrhea, rehydration, milk, electrolytes
1038 Study of the Efficacy of Hiprabovis Pneumos in front of a
Challenge Infection with Mannheimia haemolytica in Young
Calves
A. Foix, S. Casademunt, R. March
Laboratorios Hipra, S.A., Amer (Girona), Spain
242 XXV. Jubilee World Buiatrics Congress 2008
Objective: To demonstrate the protection conferred to calves infected
with M. haemolytica by a vaccine against Mannheimia haemolytica
and Histophilus somni.
Materials And Methods: The vaccine Hiprabovis Pneumos (Laboratorios
Hipra, S.A.) of M. haemolytica A1 leukotoxoid (450 IEU
per dose) and H. somni (1x10 10 bact/dose) was tested (group A, 6
calves), and compared to non-vaccinated (placebo)+ infected (group
B, 6 calves) and to non-vaccinated + non-infected (group C, 2 calves
as sentinels). The vaccine and placebo (PBS) were administered
twice, at the dose of 2 ml, separated 21 days. Groups A and B were
experimentally infected in day 42 intratracheally with 1x10 10 cfu of
M. haemolytica in 10 ml per calf. Efficacy was evaluated by
studying clinical signs (scoring system based on rectal temperature,
apathy, anorexia, nasal discharge, lung auscultation, conjunctivitis,
dyspnoea and cough), macro and microscopic lung lesions and lesion
size. No other treatments were given during the study to these
animals of 100-150 kg and of 2 months of age. Presence of
antibodies to M. haemolytica leukotoxoid was an exclusion factor.at
beginning of the study. Mean and standard deviation were calculated
for rectal temperatures, clinical signs and lung lesions score at
different days post-vaccination for each group of calves. A t-test and
a Mann-Whitney test were used for continuous and discontinuous
variables respectively (P=0.05). Animals of group C were not
included in the statistical analysis.
Results: Clinical differences between infected (A, B) and noninfected
(C) proves the success of the method of infection. The
clinical signs score (0 to 3) showed differences between groups A
and B at day 1 (p=0.06), day 4 (p=0.02) and day 7 (p=0.007). After
infection 2 calves of group B died (days 1 and 6). Mean rectal
temperature was different for groups A and B at day 1 post-challenge
(p=0.02). Percentage of pneumonic pulmonary tissue was: A =
18.1%; B = 51.2% (A-B, p=0.01); C = 13.9%, and this means a
reduction of 64.6% for the vaccinated group. The severity of
pulmonary lesions score (0 to 5) was: A = 1.8; B = 3.8 (A-B
p=0.009); C = 1.5, and this means a reduction of 52.6% for the
vaccinated group.
Conclusions: Hiprabovis Pneumos proved to be effective in front of an
experimental infection with M. haemolytica as it did reduce the clinical
symptomatology, rectal temperature, percentage and severity of
pneumonic tissue.
1039 Neutrophil Oxidative Burst in Nelore and Limousin Calves
in the First Four Months of Life
M. Costa, K. Flaiban, R. Dognani, E. Vettorato, M. Balarin, J. Lisbôa
Universidade Estadual de Londrina, Londrina, Brazil
Objectives: To study the oxidative burst of neutrophils in Nelore and
Limousin calves in Brazil during the first four months of life.
Materials and Methods: 90 healthy calves (45 Nelore; 45
Limousin) were randomly selected from two farms in Parana State,
Brazil. Blood samples were collected at 24 to 36 hours, 15, 30, 60,
90, and 120 days of life. Neutrophil oxidative burst was determined
by the non-stimulated (NS-NBT) and stimulated (S-NBT) nitroblue
tetrazolium test. The variation of positive neutrophils during the
various ages was analysed by the Krushkal-Wallis statistical test.
Comparisons between the breeds at different ages were done using
the Mann-Whitney statistical test.
Results: The percentages of reactive neutrophils to the NS-NBT test
were 2.5, 2.0, 2.0, 3.0, 3.0 and 2.0 for Nelore and 2.0, 2.75, 4.0, 4.0, 5.0
and 5.0 for Limousin calves on the above cited ages. For the S-NBT,
the values of 6.0, 6.0, 6.5, 8.0, 10.5 and 6.0 for Nelore and 6.0, 8.0, 8.5,
9.5, 15.5 and 15.0 for Limousin calves were obtained.
Conclusions: The neutrophil oxidative burst was less effective in
newborn calves increasing with age in both breeds. There was a
significant increase in the stimulated test by 90 days of age.
However, this activity decreased at 120 days in the Nelore calves.
Such an increase can be due to the immune response against
hemoparasites, which are normally encountered at this age in calves
kept under range conditions in tropical countries. Statistical
differences among the two breeds were observed at 30, 90 and 120
days in the NS-NBT and at 15, 30, 90 and 120 days in the S-NBT.
Although, Limousin calves had a higher neutrophil activity, this
difference had no practical importance in the health of the studied
calves, probably because of the low challenge observed in animals
kept on pasture and the good management practices on both farms.
Younger calves can be considered to be more susceptible to disease