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Oral and Poster Abstracts

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The level of antibodies produced in colostrum following injection of<br />

the trivalent Rota-corona virus <strong>and</strong> E. coli vaccine was evaluated to<br />

underst<strong>and</strong> the prophylaxic effect of vaccine against calf diarrhea. The<br />

number of 46 pregnant cows from industrial dairy herd were selected.<br />

A 5 mls trivalent Rota-corona virus <strong>and</strong> E. coli vaccine was injected<br />

subcutaneousely in 7 months pregnant cows. After parturition a 50 mls<br />

colostrums was collected <strong>and</strong> evaluated for antibodies using<br />

compatative ELISA method <strong>and</strong> commercial kit of Rota-corona virus<br />

<strong>and</strong> E. coli (Bio X, Belgium). Meanwhile, 27 pregnant cows which<br />

have not vaccinated were used as control group. From out of 46<br />

vaccinated cows, antibodies against E. coli was detected in 33 cases<br />

(71.7%) including one positive were 4 cases (8.7%), two positive were<br />

28 cases (60.9%) <strong>and</strong> four positive was one case (3.1%). In control<br />

group 23 cases were negative (85.2%) <strong>and</strong> one positive were 3 cases<br />

(11.1%) <strong>and</strong> two positive was one case (3.7%). From out of 46<br />

vaccinated cows, antibodies against rota virus were detected in all<br />

samples (100%) including one positive were 3 cases (6.5%), two<br />

positive were 43 cases (93.5%). For corona virus one positive were 9<br />

cases (19.5%) two positive were 34 cases (73.9%) <strong>and</strong> 3 positive were<br />

3 cases (6.5%). All control group for rota <strong>and</strong> corona antibodies were<br />

two positive. The highest <strong>and</strong> lowest inhibition percent in vaccinated<br />

cows for E. coli antibody were 85% <strong>and</strong> 1%, rotavirus were 59% <strong>and</strong><br />

20% <strong>and</strong> corona virus were 61% <strong>and</strong> 20%, respectively. Chi-Square<br />

test shows significant difference (P

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