Oral and Poster Abstracts

andomly assigned to one of five treatments. Four and two weeks
before expected calving, the heifers were injected intramuscularly 0 ml
(T0), 10 ml (T1), 20 ml (T2), 30 ml (T3), 40 ml (T4) selenium and
vitamin E respectively. The control (C) group received no supplement.
Blood samples were collected from heifers four weeks before expected
calving and at calving day. Blood samples of calves were drowning
from the jugular vein at birth and 7 days of age. Serum concentrations
of Se were determined using hydride generation Atomic absorption
Spectrophotometer. The result indicated that the serum concentration
of Se, Cu and Fe increased in T3 and T4 treatment heifers at calving
day but no change seen in serum concentration of Zn. Serum Cu
concentrations in calves of treated groups increased at birth and first
week of age but was not significantly different. The serum
concentration of Cu and Fe increased in calves of heifers given Se and
vitamin E compound esp. in T3 and T4 treatments but the serum
concentration of Zn decreased. It seems that the Zn: Cu and Zn: Fe
ratios be disturbed which lead to Zn reduction. It can be concluded that
Zn should be supplemented when more than 40 mg/Kg Dm
administered to pregnant heifers.
Key words: Zn, Cu, Se, interaction, heifers
454 Effect of Mixed Enzymes on Alfalfa Hay Nutritive Value
Using in vitro Gas Production and in situ Techniques
B. Baghbanzadeh Nobari, A. Taghizadeh, H. Janmohamadi,
S. Alijani, H. Paya, G. Moghadam
Dept. of Animal Science, Faculty of Agriculture, University of Tabriz,
Tabriz, Iran
Fibrolytic enzymes are typically cellulase that degrade cellulose or
xylanase that act on hemicellulose (Beauchemin et al., 1995). Enzymes
applied to forages immediately before incubation enhance the digestion
of dry matter (DM) and neutral detergent fiber (NDF) suggesting that
fibrolytic enzymes applied at feeding can improve digestion in
ruminants.
455 Changes of Serum Parameters in Monensin-contained Diet of
Gezel Sheep
B. Baghbanzadeh Nobari 1 , A. Rezapour 2 , P. Namavar 1 ,
G. Moghadam 2
1
Dept. of Animal Science, Faculty of Agriculture, University of
Tabriz, Tabriz, Iran
2
Dept. of Animal Science, Faculty of Agriculture, Islamic Azad
University of Tabriz, Tabriz, Iran
Five Gezel male lamb treated with 4 levels of monensin (0, 10, 30, and
60 mg/kg of diet) in a change-over design. Blood samples were taken
one hour before and 2 hrs after feeding. Serum was separated and
freezed up to analysis. Serum levels of glucose, beta-hydroxy-butyrate,
triacylglycerol, total protein, albumin (albumin/ globulin), and blood
urea nitrogen (BUN) was measured by colorimetric methods.
Considering liver health, serum activity of AST, ALP, and GGT was
measured. Ruminal fluid sample was taken for pH analysis
(ruminocynthesis). Total and differential white blood cell count and
phagocytosis test undertaken. Results of this study showed that
monensin in a level of at least 30 mg/kg of diet could effectively inhibit
acidosis occurrence. Acidosis has a negative effect on appetite; acidotic
animals have lower levels of glucose in serum. But, monensin does not
have any independent positive effect on serum glucose. Even two-folds
of this dosage had no negative effect on liver. Monensin does not any
stimulatory effect on natural immunity either independently or dosedependently.
Generally staying, monensin may be inhibiting acidosis
elated complications such as natural immune depression.
Key words: gezel, immunity, monensin, sheep
456 D-vitamin Status of Cows in Preparturient and
Postparturient Periods under Injection of Cholecalciferol
L. Yuskiv 1 , B. Kurtiak 1 , V. Vlizlo 2
1 Institute of Animal Biology UAAS, Cattle Nutrition, Lviv, Ukraine
2 Institute of Animal Biology UAAS, Scientific Center for Prion
Infections Study, Lviv, Ukraine
The aim of our work was the investigation of influence of different doses
(210 and 420 IU D 3/kg body weight) of vitamin D 3 on vitamins status of
cows in preparturient and postparturient periods under the parenteral
18 XXV. Jubilee World Buiatrics Congress 2008
injection of vitamin D 3 once before calving and three times after calving
with interval of seven days. The experiment was carried out on the three
groups of cows of Black-and-White breed, 5-6-years-old age, in a winterspring
period. The cows of first group, to which vitamin D did not
injection governed as control. To the cows of 2-nd and 3-rd groups the
vitamin D 3 was injected intramuscularly in abovementioned doses. In
blood the level of 25-hydroxyvitamin D, the concentration of calcium,
magnesium, inorganic phosphorus, the activity of total alkaline
phosphatase and its isoenzymes at 3-5 days before calving, 5-7 days and
55-60 days after calving were determined. It was established, that the
content of 25-OH D 3 in the blood serum of cows of 1-st, 2-nd and 3-rd
groups at 3-5 days before calving was correspondently: 18,75; 25,95 and
32,85 nmol/l, and at 5-7 days after calving 15,83; 20,83 and 31,88 nmol/l.
At 55-60 day after calving the concentration of 25-OH D 3 in blood serum
of cows was the greatest and varied from 20,48 till 35,0 nmol/l. Thus in
the cows blood of 2-nd and 3-rd groups before and after calving dose
dependence increase of concentration of calcium of total, protein-linked
and ultra-filtrates was observed. The concentration of magnesium in the
blood of cows of 3-rd group in the 5-7 day after calving was statistically
significantly higher about 19%, and in the 55-60 day about 6 %,
comparatively with the control. At the same time, in the blood of cows of
2-nd and 3-rd groups, comparatively with the control group, dose
dependence increase of concentration of inorganic phosphorus and
decrease of the activity of alkaline phosphatase and its isoenzymes (bone
and intestinal) was observed. Parenteral injection of cholecalciferol to
cows before and after calving maintained the level of 25-OH Vit D 3 and
effected on the exchange of mineral components of blood, that prevents
the decrease of their level in postparturient period. The character of these
changes depend on the physiological state of cows and on the doses of
vitamin D 3.
Key words: cows, prepartum, postpartum, calcium, 25-hydroxycholecalciferol
457 Clinical and Subclinical Cases of Phosphorus Deficiency and
Hypophosphatemia in Egyptian Buffaloes
H. Morsy 1 , A. Sheikh 2 , M. El-gamal 3
1
Faculty of Veterinary Medicine, Suez Canal University, Department
of Animal Nutrition, Ismailia, Egypt
2
Faculty of Veterinary Medicine, Department of Animal Nutrition,
Ismailia, Egypt
3
Faculty of Veterinary Medicine, Department of Animal Nutrition,
Cairo, Egypt
The correlationship between food intake, hypophosphatemia, blood
contents, pregnancy and health condition were done in 35 bufalloes 2-
4 months before parturation at 7-9 years old (9 healthy as a control,12
with clinical and 14 with subclincal hypophosphatemia according to
blood , serum and feed analysis and clinical examination).
Analysis of feed stuff revealed a lower level of phosphorous, a higher
level of calcium and molybdenum with a normal level of copper.
Clinical cases of hypophosphatemia showed normal temperature,
elevated pulse and respiration, sluggish rumen movement, depraved
appetite, pica, dullness, emaciation, red haemoglobin urine and pale or
sometimes icteric mucosus membrane.
The subclinical cases showed a normal temperature, pulse, respiration,
depraved appetite, dullness and sometimes locomotors disturbances.
The blood level of hemoglobin content and PCV were significantly
decreased in clinical cases only.
Serum levels of inorganic phosphorous was highly significant
decreased with a decrease of serum copper level in clinical cases and
significantly decreased of phosphorous in subclinical cases. Serum
calcium and molybdenum levels were significantly increased in clinical
cases and slightly increased in subclinical cases. Serum magnesium,
sodium, potassium and chloride showed a non significant changes in
clinical and subclinical cases.
Treatment with Sodium dibasic phosphate (60 gm diluted in 300 ml
distilled water was given 4 times within 12 hours intervals, The first
injection was intravenous, the others was subcutaneous) as well as
daily 20 ml catozal intravenous and 250 gm bran and 10 gm copper
sulphate per os in food for one week was satisfy for treatment the
clinical cases. Subclinical cases were treated with oral daily of 30 gm
sodium dibasic phosphate, 125 gm of bone meal and 2-4 kilogram of
brain for a period of one month. Correction of feed supply was helpful
and advised in both cases. It is concluded that diet, blood and serum
analysis and the general health condition were helpful for diagnosis of
clinical and subclinical hypophosphatemia in pregnant buffaloes,