Oral and Poster Abstracts

566 Somatic Cells Count of Milk in Algeria
S. Kebbal, I. Gharbi, M. Amara, S. Guemra, D. Guetarni
University of Blida, Veterinary Medicine, Algeria
For a long time used for the following sanitary and management
engineering of the milk’s cattle, count cells is been the official criteria of
the milk’s payment and the control milk. In the present work, we give a
method of somatic cells’ count to mean of particular counter “COULTER
COUNTER” which is a new application in Algeria. After calibration of
the device, an evaluation of its performances has been realised by the
determination of reliability and repeatability of analysis on samples of
quarter’s milk classed before microscopic count by the method of Prescott
& Breed. The results obtained revealed a good correlation (CC = 0, 98)
with regard to the reference method. the repeatability has showed that the
coefficient of variation don’t exceed the 5% (3,26%, 2,39% and 3,57%)
for the cellular concentrations lower than 250 x 10 3 included between 250
x 10 3 and 750 x 10 3 and superior to 750 x 10 3 Cells/ml respectively. the
reproducibility showed a coefficient of variation o 0,55%. The
performances of “COULTER COUNTER” are good and this method of
measure of cells concentration can serve for the somatic cells count of
milk with a good precision.
Key words: Mastitis, concentration, somatic cells, raw milk
567 Prevalence of Mastitis and Raw Milk Safety in Hamdallaye
Dairy Cattle (Niger)
A. Harouna 1 , C. Locatelli 1 , L. Scaccabarozzi 1 , P. Cremonesi 1 ,
V. Bronzo 1 , G. Adamou 1 , P. Boettcher 2 , G. Ruffo 1 , P. Moroni 1
1
University of Milan, Milan, Italy
2
Institute of Agricultural Biology and Biotechnology (IBBA), Milan,
Italy
A study was performed in 2007 in Hamdallaye dairy herds (Niger) to
establish the prevalence of subclinical mastitis and possible associated
risks for public health. The studied sites are organized in the cooperative
“UPROLAIT”, created by Project of Safety of Niamey Periurban
Farming and Agriculture. Seven herds participated in the study and 572
quarter milk samples were collected from 143 lactating cows from four
local breeds: Azawak, Bororo, Djelli and Goudali. Each sample
underwent bacteriological examination and automated counting of
somatic cells (SCC). Selected strains (N = 15) of Staphyloccocus aureus
isolated from the samples were subject to antimicrobial susceptibility
tests and multiplex PCR to evaluate the potential production of
enterotoxins A, C, D, G, H, I, J and L. Descriptive statistics of general
mastitis prevalence, herd prevalence and relationships between bacteria
and SCC were estimated using SPSS version 15.0. According to surveys
of dairy farmers, 33% of cows had open sores on their udders and teats.
Statistically significant differences in prevalence and SCC were
observed among pathogen species (p < 0,05). General mastitis
prevalence in Hamdallaye dairy cattle was 28,15% with: S. aureus
(10,45%); Coagulase Negative Staphylococci (CNS) (15,55%) and
environmental microrganisms (2,15%) being the most common
pathogens. Herd prevalence ranged from 2% (Toka binekani) to 18%
(Gagare) and from 4% (Bartchawal) to 39% (Toka binekani) respectively
for S. aureus and CNS. The SCC ranged from 103 to 107
cell/ml of milk. Infections were assigned to four classes, according to
the major pathogen, and the respective mean SCC ( 2,000x1000cells/ml) and clinical signs. Silver ion was
infused through intramammary route. Ten quarters of 6 cows were
infused with 20 ml silver ion solution for consecutive 3 days. Blood
samples at 0, 1, 2, 3, 5 and 7 days after intra-mammary infusion of silver
ion were collected for hematological analyses from coccyegeal vein
using K3 EDTA tube. Milk samples were aseptically collected from the
infused quarters of each cow at 0, 12, 24, 48, 72, 120 and 168 hrs after
intra-mammary infusion of silver ion. The clinical signs of mastitis in
each cow responded differently to intra-mammary infusion of silver ion.
However, significant reduction in swelling (80%), firmness of
mammary gland (100%) and clots in milk (86%) was observed in silver
ion treated quarters. Mean SCS in milk from quarters having clinical
mastitis was reduced (P