Oral and Poster Abstracts

(3.6%) and others (Bacillus spp., Nocardia spp, Candida spp.) (1.7%).
These results demonstrate a significant share of minor pathogens beside
contagious mastitis pathogens as S. aureus and S. agalactiae in mastitis
incidents in Jalisco, Mexico.
Key words: clinical and subclinical mastitis, bacterial pathogens, dairy
cattle, Staphylococcus aureus, Streptococcus agalactiae
595 Evaluating Post-Milking Teat Dip Efficacy Using Somatic
Cell Count Data
A. Bradley 1,2 , J. Breen 1,2 , P. Janowicz 3 , M. McKinzie 3 , T. Hemling 3
1
University of Bristol, School of Veterinary Science, Bristol, United
Kingdom
2
Quality Milk Management Services Ltd., Wells, United Kingdom
3
DeLaval, Kansas City, United States
Objectives: Post milking teat disinfectants are traditionally assessed
by identifying new intramammary infections (IMI) using regular
individual quarter bacteriology. Somatic cell counts (SCC) are
recognised as a proxy for IMI; quarters can be classified as “infected”
or “uninfected” and new infections defined by movements across
defined thresholds. The aim of this study was to compare the efficacy of
two post milking teat disinfectants using both bacteriology and somatic
cell counts, and to assess the utility of these two approaches.
Materials and Methods: Two teat dip formulations (DeLaval Prima
(0.5% hydrogen peroxide) and Hamra Blue (0.4% chlorhexidine)) were
compared on three farms in the UK. Four hundred and fourteen cows
were randomly allocated (ratio 1:1) to be dipped post milking with one
of the two formulations. Cows were recruited over a 20 week period;
377 cows were eligible for inclusion in the subsequent analyses. Quarter
milk samples were collected for bacteriology and SCC from all cows at
enrolment and on completion of the study. During the study, quarters
were sampled for SCC every 14 days. Bacteriology to confirm new IMIs
was performed when quarters experienced a rise in SCC from below to
above 100,000 cells/ml. All cases of clinical mastitis were sampled
during the study in order to determine the cause of IMI. New IMIs were
defined using various criteria; efficacy was evaluated using statistical
protocols recommended by the National Mastitis Council (NMC).
Results: Efficacy of the two products was compared; new IMIs were
defined by movements across the 100,000 cells/ml threshold alone, and
in combination with supporting bacteriology. Efficacy was also
evaluated by comparing clinical mastitis incidence and the change in
prevalence of IMI between entry and exit of quarters from the study.
With the exception of the change in prevalence of infection between
entry and exit from the study (due to a difference in minor pathogen
IMI), none of the measures demonstrated a significant difference
between the products. Analysis by various statistical protocols
recommended by the NMC showed the experimental product (DeLaval
Prima) was equivalent to the control product (Hamra Blue).
Conclusion: The use of SCCs, with/without the judicious use of
bacteriology offers a viable, cost effective alternative to the extensive
use of bacteriology alone in assessing post-milking teat dip efficacy.
596 The Effectiveness of Cefalosporine+Kanamicine and
Ampiciline Gentamicine + Dexametazone in the Treatment of
Clinical Mastitis in the Livestock Bovine Milk in Mexico
M. Posadas, L. Matus, O. Blanco, B. Pena
Facultad de Medicina Veterinaria y Zootecnia-UNAM, Animal
production, México DF, Mexico
The present study was carried in Tizayuca Hidalgo, Mexico during July
to September in order to determining the effectiveness of the treatment
with the combination cefalexine monohydrate, kanamicine sulfate and
ampiciline - gentamicine + dexametazone. We administered by the
intrammary road for the solution of problems of clinical mastitis in
bovine producing of milk. The sample was took in a population of 50
animals diagnosed with clinical mastitis and that they presented
alterations in the milk besides that they obtained a grade 2 in test of
California (CMT), which were integrated in two groups of 25 glands
distributed randomly.The control group (A) it was conformed by 25
glands affected which was administered cefalexine monohydrate +
kanamicine sulfate during 2 days for a intramamary road.The witness
group (B) was conformed by 21 affected glands, to which was
administered: ampiciline - gentamicine + dexametazone for a
intramamary road with it forms to their medical prescription. They took
samples of milk of the glands affected to carry out the bacteriological
64 XXV. Jubilee World Buiatrics Congress 2008
exam and to know the etiology.The isolated agents of the sample of milk
of the two groups “A” and “B” was the following ones: Streptococcus
agalactiae, Staphtlococcus aureus, Corynebacterium pseudotuberculosis,
Pasterella spp., Moraxella spp., Eschericha coli, Staphylococcus
epidermidis, Staphylococcus, saprophyticus, Serratia spp., Streptococcus
uberis, Citrobacter spp., coagulase negative Staphylococci,
Actinobacillus salpingitidis, Staphylococcus intermedius and Aeromona
hydrophila. The results demonstrated that the combination cefalexine
monohydrate and kanamicine sulfate, it had an effectiveness of 88% to
control the presentation of clinical mastitis of the group “A” in
comparison with the group “B” that was of 66%. In the statistical analysis
they were not significant differences (P=.065>0.05). As for the
presentation of alterations in the milk, the animals of the group “A” they
recovered in 86% while the group “B” was of 76%. With regard to the
test of California for the group “A” 82% of the glands didn’t present
reaction some after the treatment while in the group “B” was of 68%,
what allows to deduce that the cefalexine monohydrate and kanamicine
sulfate is a highly advisable product for the treatment of clinical mastitis.
597 Effect of Anhydrous Cephalonium-Based Drug Indicated for
Bovine Mastitis Treatment during the Dry Period on
Phagocytosis Rates
C. Batista, M. Blagitz, M. Azedo, C. Stricagnolo,
M.C. Sucupira, A.M. Della Libera
Faculdade de Medicina Veterinaria da Universidade de Sao Paulo,
Clinica Medica, Sao Paulo, Brazil
The dry period is considered a very vulnerable phase of lactation to
mastitis and the phagocytosis mechanisms are fundamental for this
period. The antibiotic therapy used for the treatment of dry cows acts
on the elimination of existing intramammary infections, avoiding new
infections and decreasing its incidence on the next lactation. An
efficient drug must contain an adequate antimicrobial component in
harmony with other eventual components and must be harmless to the
organism’s defense mechanisms. Twenty-one paired samples, negative
to bacteriological tests, were used to evaluate the influence of
Cepravin ® , an anhydrous cephalonium-based drug indicated for the
treatment of mastitis during the dry period, on the phagocytic function
of milk leukocytes. In order to evaluate the cellular activity, leukocytes
were isolated in adequate quality (viability) and quantity
(concentration) and submitted to in vitro phagocytosis test with
Zymosan (Saccaromyces cerevisae cell wall), after exposition to a
solution containing the drug. The average phagocytosis rate for cells
submitted to the treatment (64.43% ± 15.10) did not differ in relation to
the average phagocytosis rate for the control group (58,33% ± 12,00).
In addition, the coefficient of variation of phagocytosis rate obtained
from cells submitted to the treatment (23.44) was not different from the
coeficient found in control samples (20.57). The results suggest that the
treatment, besides the antibiotic activity, does not interfere in the
phagocytic function of milk leukocytes, which is fundamental for the
defense of mammary gland, mainly during the dry period.
Key words: mastitis, treatment, phagocytosis, dry period
598 Microbiological Evaluation, Somatic Cell and Differential
Leukocytes Count in Relation to California Mastitis Test in
Sante In’s Ewes
M. Blagitz 1 , F. Souza 1 , V. Gomez 1 , C. Batista 1 , M. Azedo 1 ,
C. Strincagnolo 1 , N. Benites 2 , P. Melville 2 , M.C. Sucupira 1 ,
A. M. Della Libera 1
1 Faculty of Veterinary Medicine and Animal Husbandry FMVZ-USP,
Clinica Medica, Sao Paulo, Brazil
2 Faculdade de Medicina Veterinaria da Universidade de Sao Paulo,
Preventive Medicine and Public Health, Sao ppaulo, Brazil
To evaluate the variation in microbiological profile, total somatic cell
(SCC) and differential leukocytes counts (DSCC) in milk of Santa Ins
ewes according to CMT scores, 170 milk samples were examined for
microscopic somatic cell count (SCC), differential leukocytes count
(DSCC), microbiological examination and their relationship with
California mastitis test (CMT). On the 24.21% bacteriologically
positive samples, 86.96% were coagulase negative staphylococci
(CNS), and 13.04% were Streptococcus dysgalactiae. The mean (±
Standard deviation) logarithm SCC of uninfected sheep’s gland was
5.01 (±0.87) and 5.93 (±0.94) (p