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electrochemical properties of the analyte under analysis. Recent studies have demonstrated the capacity of these devices to detect and quantify micro- and submicro molar concentrations of both redox mediators (id. Metal-organic compounds), commonly used in electrochemical biosensors, and other molecules. The applications of these devices in Veterinary Medicine are: quantitative determination of drugs, steroids, pathogenic bacteria, toxins, Genetically Modified Organisms, Polychlorinated biphenils, and pesticides. In this study the copper ion (Cu 2+ ), has been determined as scientific literature reports both high serum Cu concentrations in molluscicide polluted water intoxication in bovine and ovine species, and low serum concentrations in calves with neuromuscular problems. An electrochemical nano-structured biosensor, with both high ratio Signal/Noise (S/N), and very high selectivity, has been utilised for determination of Cu 2+ in a water matrix. Materials and methods: A nano-structured electrochemical biosensor, ultra sensitive and highly accurate with a wide spectrum of application, was planned and developed in 2005-2007 years by Nano- SIMS s.r.l. by Nano Imprinting Lithography (NIL) on polymeric substrate, and utilised as a working electrode in electro analytical technique of cyclic voltammetery. This biosensor was used for ultra trace quantitative determination of Cu 2+ in water matrix has been developed. Results: This method, using a specific Cu biosensor, has allowed the determination of a very low Cu concentration of 1,2 x 10 7 ions/nL in water matrix. Conclusions: The development of this technology allows both the specific chemical programming of the biosensor, to assure the very high selectivity and specificity of the analyte to be determined, as well as the use of the biosensor in low cost devices for field applications. It is important to highlight the usage of this biosensor both regarding quality and safety of food and mass screening, and for the ultra trace quantitative determination of numerous other analytes, and for notable potential applications in Veterinary Medicine which will be studied in detail, in the near future, particularly in bovine and ovine species. Key words: nanotechnology, biosensor, copper, bovine and ovine species. 1242 Diagnostic Values of Haptoglobin and Serum Amyloid a Concentrations in Bovine Heart Diseases A. Rezakhani, S. Nazifi, A. Moaddeli, M. Zarifi School of Veterinary Medicine, Shiraz University, Clinical Sciences, Shiraz, Iran Congenital and acquired heart diseases have been reported in cattle. Acquired cardiac problems can be manifested as murmurs or arrhythmias. The prevalence of cardiac murmurs and arrhythmias among clinically healthy dairy cattle has been under investigation in our school for the past 5 years. The prevalence of valvular cysts which can produce cardiac murmurs has also been reported in our institution. Various methods of diagnostic tools have been used for diagnosis of heart diseases in animals. This study was carried out to find a possible relationship between cardiac murmurs and the concentration of acute phase proteins (haptoglobin and serum amyloid A) in dairy cattle population. Two hundred dairy cattle of different ages from four dairy farms were examined by auscultation on the base of method used by Rezakhani and Zarifi (2007). 77 out of these 200 cattle had various types of cardiac murmurs. Cases of pericarditis and endocarditis referred to our school were taken into this study for comparison. Twenty normal cattle without any sign of heart disease and cardiac murmurs were taken as a control group. Blood samples were taken from all cattle with cardiac murmurs, from cases referred to school teaching hospital and from the control group for determination of haptoglobin and serum amyloid A plus CBC. Statistical analysis carried out on the findings of this study showed that there was significant difference in the concentration of these two acute phase proteins in endocarditis and pericarditis group as compared with the control group and the clinically normal cattle with cardiac murmurs (P 0.05). The findings also showed that there was significant difference in CBC in cases with enocarditis and pericarditis as compared with group having cardiac murmurs and the control group (P< 0.05). This study showed that acute phase proteins 308 XXV. Jubilee World Buiatrics Congress 2008 measurement can not be used for the differentiation of functional from pathological murmurs. Key words: heart diseases, murmurs, cattle, haptoglobin, serum amyloid A 1243 Auditory Evoked Response in the Clinically Normal Cows: Middle Latency Components A. Pomianowski, Z. Kuleta, M. Kwiatkowska University of Warmia and Mazury, Department of Internal Medicine, Olsztyn, Poland Objectives of study: Our objective was to record the middle latency components (10 to 60 ms) of the auditory-evoked response in clinically normal mature dairy cows. Materials and methods: To detect the middle latency response (MLR), a series of suprathreshold tone pips was presented monoaurally to nonanesthetized cows (n = 6 mixed breed) at a stimulus frequency 1 kHz. Stimulus intensity tone pips were presented at 80 dB hearing level (nHL), introduced through insert earphone tranducer. Contralateral masking noise at 50 dB nHL was used. Filtered electroencephalic activity during the first 100 ms after each tone pip stimulation was recorded and averaged digitally for 2000 consecutive stimuli. Results: The response pattern in the cows was repeatable polyphasic configuration characterized by 4 major waves. Peaks of the waves were labelled, using a method developed in human beings. Mean latency ranges for the positive (P) or negative (N) peaks were 11,2 to 12,6 ms (Na), 17,5 to 18,3 ms (Pa), 27,1 to 28,8 ms (Nb) and 43,4 to 45,4 ms (Pb). Conclusions: Configuration of used parameters allows to detect MLR in cows. They reflect the functional integrity of the thalamus-cortical region and primary auditory cortex. Measurement of peak latencies provides information to supplement the results of standard neurologic examination and can be useful tool for monitoring anaesthesia and analgesia. Key words: middle latency responses, cows 1244 A Simplified Method of Estimation of Plasma Fibrinogen Concentration without Heat Precipitation A. Roussel 1 , J. Osterstock 1 , A. Bouchant 1 , E. Calzamiglia 1 , K. Russell 2 1 Texas A&M University, Large Animal Clinical Sciences, College Station, United States 2 Texas A&M University, Veterinary Pathobiology, College Station, United States Plasma fibrinogen concentration (FC) determined by heat precipitation (HP) has been used to support the diagnosis of inflammatory disease in cattle. This method requires a heated water bath which is not universally available in practice. Our objective was to evaluate an alternative method of FC measurement which does not require HP. We studied 75 cattle (sick and clinically normal) admitted to the Texas Veterinary Medical Center. Blood samples were collected into plain vacuum tubes and vacuum tubes containing EDTA. Blood from the EDTA-containing tubes was centrifuged for 4 minutes in 2 micro-hematocrit tubes within 60 minutes of collection, and the plasma protein concentration was estimated from one of the tubes using light refractometry. The second microhematocrit tube was placed in a water bath heated to 56° C for 5 minutes to precipitate the fibrinogen and then centrifuged. The serum protein concentration on this sample was estimated using refractometry. The difference in the protein concentration between the two micro-hematocrit tubes is the FC determined by HP. After 1 hour at ambient indoor temperature, the plain tube was centrifuged, and serum protein concentration was estimated using refractometry. The FC was then estimated by subtracting the serum protein concentration from the plasma protein concentration. Summary statistics were calculated and means were compared by method using a paired t-test and by operator using a t-test. The FC was also compared between the 2 methods using linear mixed-effects models. Mean FC from HP was 441 mg/dl (S.D. 142.0 mg/dl) and mean FC from ambient temperature clotting was 412 mg/dl (S.D. 155.4 mg/dl). The difference between the FC measured by HP and ambient temperature clotting was 0 for 22 cattle, 100 mg/dl for 42 cattle and 200 mg/dl for 11 cattle. Comparison of results obtained using HP between different operators revealed a significant difference
etween mean results for the 2 operators in this study (P = 0.003). Results from the mixed-effects model indicated that results of the two methods were not significantly different (P = 0.66). However, a significant effect associated with operator (P = 0.04) and a significant method-by-operator interaction (P = 0.02) were detected. The results of this model demonstrate that estimation of FC using HP and the ambient temperature clotting yields similar results, but differences in the results obtained by different operators may affect results of the HP method. 1245 Seasonal and Regional Differences of Iodine Concentration in Raw Cow, Sheep, and Goat Milk in Slovakia I. Paulikova, H. Seidel, O. Nagy, G. Kovac University of Veterinary Medicine, Kosice, Slovakia Aim of this work was to map iodine content in raw milk of dairy cows, sheep, and goats regardless breed, production, and reproduction cycle and to compare milk iodine levels in various Slovakian regions, as well milk iodine concentrations during winter and summer feeding periods. The samples were taken manually after washing of quarters and halves, resp., without use of iodine disinfectant. The animals from 32 farms (dairy cows - 23, sheep - 7, goats - 2) were divided into four groups according to the regions – Western, Middle, Eastern, and Northern Slovakia. We compared also milk iodine concentrations recorded during the summer (1 May - 31 October) and winter (1 November - 30 April) feeding periods. The milk iodine concentrations were determined by photometric method for analysis of trace iodine based on catalytic reaction NO 2 - /SCN - . Milk iodine concentrations were determined in 457 samples of raw milk from dairy cows, 79 samples of sheep, and 17 samples of goat milk collected in various Slovakian regions from 2002 to 2007. Among all the 457 samples of bovine milk, iodine content below 50 g.l -1 was recorded in 113 samples (29.94 %); 294 samples (64.33 %) ranged between 50 and 200 g.l -1 ; 19 samples (4.16 %) from 200 to 500 g.l -1 ; 17 samples (3.72 %) between 500 and 1000 g.l -1 , and 13 samples (2.85 %) showed milk iodine concentrations over 1000 g.l - 1 . Concentrations in the Slovakian regions showed the highest values in the Western, then Middle and Eastern Slovakia, and the lowest values in the Northern Slovakia (p < 0.05, p < 0.01). However, the results showed large variability within both regions and farms. In sheep and goat milk samples, we found milk iodine concentrations below 80 g.l -1 in 49 sheep (62 %) and in 6 goats below 60 g.l -1 (37.5 %), which are indicative of iodine deficiency. When comparing seasonal differences, sheep and goat milk was higher in the iodine content during the winter feeding period, however, in dairy cows we recorded opposite ratio. Except goat milk (p < 0.01) these difference were not significant. Key words: iodine, raw milk, cattle, sheep, goat 1246 Family Dairy Production Process in Mexico - a Case Study R. J. Jiménez 1 , F. P. Alonso 1 , L. H. García 2 , V. O. Espinosa 1 , A. P. Alonso 1 , L. P. Brunett 3 , J. G. Meléndez 1 , J. T. Cruz 1 1 Facultad de Medicina Veterinaria y Zootecnia - UNAM, Departamento de Economía, Administración y Desarrollo Rural, Mexico City, Mexico 2 Universidad Autónoma Metroopolitana Unidad Xochimilco, Producción Agrícola y Animal, Mexico City, Mexico 3 UAEM, Facultad de Medicina Veterinaria y Zootecnia, Mexico State, Mexico The aim of this project was to describe the family dairy process production in order to find proposals to improve the production of this system. The project was made in 20-production units in four communities of Maravatio, Michocan, Mexico. Through questionnaires, semi-structured interviews and participative observation was gotten information about the productive process. It was made a frequencies study and the activities of the process were described. Results obtained showed that cow ownership was influenced mainly by Holstein-Friesian cross. They have herds less to 20 cows that are maintained in semi-extensive system in summer and feed-lot system in winter. In technology, all of them have preventive medicine schedules; 14 producers performed artificial insemination, and only 7 producers have mechanical milking. In the feeding mainly are used holding crop residues, maize, sorghum, commercial balanced food and grazing. Family labour is used on production process in 95%. Four main activities were identified: facilities cleaning, milking, milk delivery and feeding. It was observed improper techniques in: 1) the manual milking that have a negative influence on the quality product; 2) the feeding, due to the inefficient use of resources; and 3) the marketing because of the entrepreneurs who reduced the producers’ income. However, some producers have adapted technology which let them to improve their system. The competitiveness and permanence in a global market is based on the quality product and the efficient use of sources. Furthermore, the Maravatio’s family diary production has both weakness, if it does not improve, it would be out of commercial market. This work was supported by Program for technological Research and Innovation Projects of the National autonomous University of Mexico (PAPIIT IN304307). Key words: family dairy, Mexico, process production 1247 Effect of Three Different Phytotherapics on Bovine Skin Wound Healing Process L. Lipinski 1 , A. Wouk 2 , N. Lemos da Silva 3 , D. Perotto 3 , R. Ollhoff 4 1 Universidade Federal do Paraná, Veterinary Clinical Sciences, Curitiba, Brazil 2 Universidade Federal do Paraná, Veterinary Clinical Sciences, Curitiba, Brazil 3 Instituto Agronômico do Paraná, Ponta Grossa, Brazil 4 Pontifícia Universidade Católica do Paraná, Ciencia Animal, Sao José dos Pinhais, Brazil Objectives: The present study aimed to compare the effect of the bark of Schinus therebentifolius, the sawdust of Tabebuia avellanedae and leaves and branches of Caesaria sylvestris, all native brazilian trees, on the wound healing process of the bovine skin by second intention. Material and methods: The skin of 11 Purun heifers was surgically removed with a 2cm - punch on 4 locations on both sides of the lombar region, on a parallel line 8 cm from the vertebral column and treated topically daily for 17 days with the decoctus of the three plants. One lesion on each side remained as control and was rinsed with saline solution. One row was randomly chosen to be used for the histopathological excisions. The lesion was macroscopically evaluated at 5 times and the microscopic aspect (inflammatory response, young and mature granulation tissue) was evaluated twice (day 6 and 16). The centripetal cicatrization was measured drawing the border line of the lesion on a transparent sheet and calculating the area beneath. All animals were kept in individual stables with a previous 10 days adaption and received the same balanced feed. The macroscopic aspect was judged always by the same person who applied 3 different degrees (1 = bad, 2 = regular, 3 = good), considering local hemorrhage, presence of coagula, crusts, granulation tissue, epithelialization, presence of exudate. Results and discussion: Lesions treated with Tabebuia were better evaluated probably due to his anti-inflammatory properties, although histopathology with all treatments showed no influence over inflammation. Lesions treated with Caesaria initially did not differ from Schinus, but could not be differentiated subsequently from the control. Lesions treated with Schinus had a more dry and irregular aspect, probably due to the effect of tannines. The cicatrization area did not differ at any time from the control, but Tabebuia treated lesions showed a statistically measured tendency of faster improvement. The histopathological examination revealed a positive effect of Tabebuia and Schinus over fibroplasia. Conclusion: The phytoterapic extracted from Tabebuia avellanedae and Schinus therebentifolius improved cicatrization whereas Caesaria sylvestris showed a beneficial effect only at the beginning of the healing process. Phytoterapics could be used to promote the healing process as an alternative to disinfectants i.e. in organic farming systems. Key words: phytotherapy, wound, healing, bovine 1248 Selenium Profile in Blood and Hair from Cattle, Environmentally Exposed to Selenium around Isfahan Ceramic Industry, Iran M. Pourjafar 1 , H. Sateri 2 1 Shahrekord University, Clinical Sciences, Shahrekord, Iran 2 Islamic Azad University of Shahrekord, Shahrekord, Iran Miscellaneous 309
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130. évfolyam Különszám II. Vol
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The World Buiatrics Congress (WBC)
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lood and liver tissue. Absolute vit
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RNS causes increased lipid peroxida
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An experiment was conducted to stud
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2 Hungarian Institute of Agricultur
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and plasma HCO 3 - increased from H
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Materials and Methods: Serum sample
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vitamin A stores in liver, the hypo
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andomly assigned to one of five tre
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observed in group II of the animals
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The aim of the monitoring was to co
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health group (HG) categories for an
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lactating cows is sparse. Lotan and
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491 Effect of Acarbose on Milk Yiel
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499 Secondary Hepatogenous Photosen
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they all died in a two to three day
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and HCO 3 level. The concentration
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amount of saponins was observed in
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Water was offered ad-libitum. Feed
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Se, I and Co) more than 500 blood s
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541 Variability of Blood Profile an
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evaluated, highly significantly gre
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we measured sensitivity of the enti
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were 0.17; 0.13 °C (Max.: 0.73). D
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calving pregnancy period were the b
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2 University of Nottingham, Departm
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animals) was treated with 5 mg enro
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In dairying, good management practi
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Somatic cell count (SCC) is one of
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This investigation was run in some
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eproductive data showed that cows f
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(3.6%) and others (Bacillus spp., N
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602 Coagulase Gene Polymorphism of
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2 Institute of Veterinary Bacteriol
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an important part of the pathogens
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4 Universidade de Sao Paulo, Clinic
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incubated for one hour at 37 oC. Af
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In Belgium, an increasing amount of
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Conclusion: It can be concluded tha
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calves recovered after 4 to 10 days
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disease. Thus losses were estimated
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635 Prevalence of the Pulmonary Les
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Conclusions The sanitation program
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Italy. After the sample examination
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Results: The cows sera were collect
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(Mb) as co-infecting agent is becom
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distributed over 13 different fatte
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main infection sources to the urban
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686 Characterisation of Cattle Orig
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using EPFC. The intradermal tubercu
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three cattle farms each. Leptospiro
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(cELISA) only to definitively confi
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1 Islamic Azad University of Shahre
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Financial support: CNPq, CAPES, FIN
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731 Detection and Investigation on
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necropsied, tissue samples were tak
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aseline values. The mean HCO 3, PCO
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immunization with either the curren
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decrease in animal aggregation in p
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747 Effect of Dietary Supplementati
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755 Study of Pulmonary Adenomatosis
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1 University of Erciyes, Faculty of
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2006 was observed a small decreasin
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for O69 strain), and clinical signs
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2 University of Veterinary Medicine
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794 Serum Haptoglobin and Fibrinoge
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complete random design (CRD). The r
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- Ca, Mg, K, and Ca:P ratio were lo
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818 Cerebral Coenurosis in a Goat:
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concentrations of the goat milk, in
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was significant and showed that bre
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cm long and 1/48 cm diameter. Middl
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circular external skeletal fixator
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847 Llama Anesthesia for Orthopedic
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28 Preliminary Results of a General
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Israeli commercial dairy herds cons
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109 Association between Johne’s D
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195 Changes in Plasma Beta-Carotene
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In the last year 2006, in the perio
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serious economic concern in the fat
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was a cumulative incidence rate of
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The level of antibodies produced in
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1096 Determination of Risk Indicato
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ParaCheck, CSL/Biocor, Australia (C
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1111 The Prevalence of Hock Lesions
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Objectives: To evaluate two differe
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1126 Relationships between Paratube
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1134 A Comparison between Two Calf
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The present work aimed to investiga
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University College Dublin, School o
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214 Cystic Ovarian Diseases in Dair
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inseminator were tested in regressi
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University of Nottingham, Animal He
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tissue (8,6 cm 2 ) was twice as lar
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libido in unmotivated males. Simila
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groups according to their diameter
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improve embryonic development are m
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in each sample). On day 0, 104 cows
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1 Warsaw University of Life Science
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and durable spermatozoa. Within the
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motility and CM integrity (p=0.869
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the median number of attempted moun
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909 The effect of “GnRH” and
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3 COOPEVOLIA, Centre Zootechnique,
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923 Production of Bovine Blackleg V
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Institute of Physiology and Sanocre
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2 University College Dublin, Animal
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(I.M.) 1 mg of estradiol cipionate
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Lameness results in major economic
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methods; ANOVA (with binary classif
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Initially two radiographs were obta
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number of positive faecal samples d
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Pfizer Animal Health, Veterinary Me
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Key words: calf, active immunity, p
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1 University of Camerino, Veterinar
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vaccinated (Group II) with Pastobov
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# Antigens Association in diarrhea
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1015 Prevalence of Eimeria Infectio
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group showed higher serum lactate c
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thymus in calves and heifers with c
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concentrations of calves with umbil
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The goal of this paper is to expose
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187 Cooling Strategies to Improve M
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inspections, carried out by specifi
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The origin of Hungarian Grey cattle
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University of Warmia and Mazury in
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POSTER ABSTRACTS 1225 The Effect of
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multiple fracture (n=1, 1.3%). The
Page 260 and 261: position. A fusiform skin incision
Page 262 and 263: Conclusions: It is the first presen
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Page 266 and 267: of 101.55±4.94 mmHg. Afterwards th
Page 268 and 269: In Mexico, there is a wide variety
Page 270 and 271: Cardiovascular disorders are common
Page 272 and 273: Conclusion: The total contamination
Page 274 and 275: Material and methods: The trial inc
Page 276 and 277: detection of M. wenyonii infection.
Page 278 and 279: 390 Study on Attenuation Mechanisms
Page 280 and 281: POSTER ABSTRACTS 1152 Detection of
Page 282 and 283: digestion. It works only on Apicomp
Page 284 and 285: determined. In addition the surface
Page 286 and 287: Africa and Europe, which causes mor
Page 288 and 289: Results: On D0, the animals include
Page 290 and 291: addition of mineral salt, during 12
Page 292 and 293: digestion could be improved with im
Page 294 and 295: Ltd., Blandford Forum, UK). The air
Page 296 and 297: cattle ocular squamous cell carcino
Page 298 and 299: pharmacokinetic/pharmacodynamic (PK
Page 300 and 301: Bovine Immunodeficiency Virus (BIV)
Page 302 and 303: significantly lower in groups vacci
Page 304 and 305: 5.00+0.67 and 4.94+0.15 mEq/l in th
Page 306 and 307: potable water and food, avoiding st
Page 308 and 309: equal distributions at 08:00 and 16
Page 312 and 313: Selenium was discovered as an essen
Page 314 and 315: growth by measuring BW at 15-day in
Page 316 and 317: influence of different risk factors
Page 318 and 319: performances of primiparous cows ar
Page 320 and 321: anaerobes. Aerobic bacteria isolate
Page 322 and 323: P4 12 to 24 hours before calving is
Page 324 and 325: exists about Mb prevalence in dairy
Page 326 and 327: frequency of animals with BoHV anti
Page 328 and 329: esults, supported by a decrease in
Page 330 and 331: international drivers of change. Th
Page 332 and 333: The performance of two groups of no
Page 334 and 335: spasm is accompanied by kyphosis, w
Page 336 and 337: occurrence of a crisis of stiffness
Page 338 and 339: Notices 336 XXV. Jubilee World Buia
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