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The present work aimed to investigate nitric oxide (NO) and hydrogen peroxide (H 2O 2) production in monocyte-macrophage lineage from dairy cows naturally infected with bovine leukemia virus. Thus, 138 Holstein cows were casually sera tested by agar gel immunodiffusion (AGID) and the leukocyte profile was performed to discriminate the distinct groups known as alymphocytotic (AL) and persistent lymphocytosis (PL). The results on serologic test showed 45.65% positive dairy cows. From these positive animals 17.46% presented LP. From these cows, 30 animals were selected and divided equally in 3 groups (negative, AL and LP). The mononuclear cells from peripheral blood (PBMC) were isolated by gradient density centrifugation. The PBMC were incubated overnight and then the adherent cells were removed. Thus, 2x10 6 cells/ml monocytemacrophage lineage were plated per well for NO and H 2O 2 determination. NO synthesis in cell cultures was measured by Griess method and the H 2O 2 were analyzed using a simple colorimetric method with and without phorbol 12-myristate, 13-acetate (PMA). The median of H 2O 2 production from LP (28.98 nmol/mL) (p= 0.001) animals were higher than those observed from negative (SN) (6.74 nmol/mL) and AL (9.49 nmol/mL). The same were seen in H 2O 2 values with PMA stimulus (p
Material and Methods: The authors examinated post mortem 3372 bovine in the 356 herds from January 2001 to December 2007 and collected informations from farmers and veterinarians. The study population included 25838 cattle, The main causes of deaths were classificated in eight categories: respiratory disorders,digestive disorders,calving disorders,metabolic disorders,accidents,udder/teat disorders,other known reasons and unknown reason. Data collected was analysed and compared. Results: Table 1 report the mortality rate in years from 2001 to 2007. Table 1. Deaths cattle and mortality rate 2001 2002 2003 2004 2005 2006 2007 N° deaths 496 492 447 446 498 491 502 mortality 1,91% 1,90% 1,73% 1,73% 1,92% 1,90% 1,94% rate We found a higher mortality in older dairy cows (2007). The mortality rate rise from 3,41% in dairy cows 3-5 years old to 3,84% in dairy cows 5-8 years old and 4,34% in dairy cows than 8 years older.In the beef breeds ( Piemontese ) the mortality rate is higher in cows 3-5 years old (1,63%) and cows 8 years older ( 1,67%) than cows 5-8 years old ( 1,29%).The table 2 report the percentages to each group of death causes on dairy cows and beef breed cows. Table 2. Causes of death on dairy cow and beef breed cow mortality (%). Respi- Calving Meta- Accid- Udder Diges- Other Unratory dis- bolic ents /teat tive cause known disor- order disor- disor- disor causes ders ders ders ders Dairy 6 5 24 7 13 7 10 28 cows Beef 7 25 4 3 2 10 18 31 breeds cows The inter-herd variability was significant, with mortality rates running between 0 and 8%.We found that 44% of dairy cow deaths occurred within the first four weeeks of the lactation.All cattle twenty four months older (1472 deaths) were tested for Bovine Spongiform Encephalopathy (BSE) and were negative. Conclusions: We don’t found significant difference between the mortality rate in small and large herds. The quality of management result the major discriminant to reduce deaths cattle rate.A good practice breeeding has a strong positive impact on animal health, welfare and mortality. Key words: cows, cattle, mortality rate 1146 Effect of Bovine Respiratory Disease on Growth and Carcass Quality during Early and Late Finishing Periods Using Treatment Records and Lung Lesions Scoring in French Feedlot Cattle F. Corbiere 1 , C. Lacroux 1 , R. Besse 1 , R. Chevalier 2 , PR. Cloet 2 , F. Schelcher 1 1 National Veterinary School of Toulouse, Pathology of Ruminants, Toulouse, France 2 Schering Plough Vétérinaire, Animal Health Division, Levallois Perret, France Aims: Evaluate the effect of bovine respiratory disease (BRD) and lung lesions on growth and carcass quality during early and late finishing periods in French feedlot cattle. Material and Methods: 407 Blonde d’Aquitaine five to seven months old male beef calves from a selection unit were monitored. Weight was recorded at entry (day one) and monthly until slaughtering after an average of 240 days on feed. The rectal temperature and clinical signs of BRD were recorded daily during the first 3 weeks. Onward, the rectal temperature was recorded only for calves with general or respiratory clinical signs. A treatment for BRD was initiated if rectal temperature was >40°C. At slaughter, the occurrence and extent of lung lesions were recorded and scored using a standardized scale and carcass quality was evaluated according to the EUROP scale. Associations between dependant variables (mean daily weight gain (ADG) and EUROP score) and the presence and extent of lung lesions or treatment for BRD were analyzed using linear mixed regression models adjusted for potential confounders. Results: Clinical BRD occurred in 52.8% of calves, mostly (77.6 %) during the first month on feed but mortality due to BRD remained low (0.48%). At slaughter, lung lesions were observed in 46.9% of calves, mostly in cranial pulmonary lobes. The prevalence of lung lesions was not significantly different between animals never treated for BRD, animals treated once and animals treated twice or more (p=0.53). The overall ADG was 1553g ± 155g. Treatment for BRD was associated with a 177g reduction in ADG (p
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130. évfolyam Különszám II. Vol
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The World Buiatrics Congress (WBC)
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lood and liver tissue. Absolute vit
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RNS causes increased lipid peroxida
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An experiment was conducted to stud
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2 Hungarian Institute of Agricultur
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and plasma HCO 3 - increased from H
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Materials and Methods: Serum sample
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vitamin A stores in liver, the hypo
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andomly assigned to one of five tre
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observed in group II of the animals
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The aim of the monitoring was to co
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health group (HG) categories for an
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lactating cows is sparse. Lotan and
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491 Effect of Acarbose on Milk Yiel
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499 Secondary Hepatogenous Photosen
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they all died in a two to three day
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and HCO 3 level. The concentration
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amount of saponins was observed in
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Water was offered ad-libitum. Feed
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Se, I and Co) more than 500 blood s
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541 Variability of Blood Profile an
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evaluated, highly significantly gre
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we measured sensitivity of the enti
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were 0.17; 0.13 °C (Max.: 0.73). D
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calving pregnancy period were the b
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2 University of Nottingham, Departm
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animals) was treated with 5 mg enro
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In dairying, good management practi
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Somatic cell count (SCC) is one of
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This investigation was run in some
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eproductive data showed that cows f
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(3.6%) and others (Bacillus spp., N
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602 Coagulase Gene Polymorphism of
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2 Institute of Veterinary Bacteriol
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an important part of the pathogens
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4 Universidade de Sao Paulo, Clinic
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incubated for one hour at 37 oC. Af
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In Belgium, an increasing amount of
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Conclusion: It can be concluded tha
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calves recovered after 4 to 10 days
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disease. Thus losses were estimated
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635 Prevalence of the Pulmonary Les
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Conclusions The sanitation program
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Italy. After the sample examination
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Results: The cows sera were collect
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(Mb) as co-infecting agent is becom
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distributed over 13 different fatte
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main infection sources to the urban
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686 Characterisation of Cattle Orig
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using EPFC. The intradermal tubercu
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three cattle farms each. Leptospiro
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(cELISA) only to definitively confi
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1 Islamic Azad University of Shahre
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Financial support: CNPq, CAPES, FIN
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731 Detection and Investigation on
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necropsied, tissue samples were tak
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aseline values. The mean HCO 3, PCO
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immunization with either the curren
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decrease in animal aggregation in p
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747 Effect of Dietary Supplementati
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755 Study of Pulmonary Adenomatosis
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1 University of Erciyes, Faculty of
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2006 was observed a small decreasin
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Page 132 and 133: 2 University of Veterinary Medicine
Page 134 and 135: 794 Serum Haptoglobin and Fibrinoge
Page 136 and 137: complete random design (CRD). The r
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Page 142 and 143: concentrations of the goat milk, in
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Page 146 and 147: cm long and 1/48 cm diameter. Middl
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Page 150 and 151: 847 Llama Anesthesia for Orthopedic
Page 152 and 153: 28 Preliminary Results of a General
Page 154 and 155: Israeli commercial dairy herds cons
Page 156 and 157: 109 Association between Johne’s D
Page 158 and 159: 195 Changes in Plasma Beta-Carotene
Page 160 and 161: In the last year 2006, in the perio
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Page 166 and 167: The level of antibodies produced in
Page 168 and 169: 1096 Determination of Risk Indicato
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Page 172 and 173: 1111 The Prevalence of Hock Lesions
Page 174 and 175: Objectives: To evaluate two differe
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Page 178 and 179: 1134 A Comparison between Two Calf
Page 182 and 183: University College Dublin, School o
Page 184 and 185: 214 Cystic Ovarian Diseases in Dair
Page 186 and 187: inseminator were tested in regressi
Page 188 and 189: University of Nottingham, Animal He
Page 190 and 191: tissue (8,6 cm 2 ) was twice as lar
Page 192 and 193: libido in unmotivated males. Simila
Page 194 and 195: groups according to their diameter
Page 196 and 197: improve embryonic development are m
Page 198 and 199: in each sample). On day 0, 104 cows
Page 200 and 201: 1 Warsaw University of Life Science
Page 202 and 203: and durable spermatozoa. Within the
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Page 206 and 207: the median number of attempted moun
Page 208 and 209: 909 The effect of “GnRH” and
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Page 212 and 213: 923 Production of Bovine Blackleg V
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Page 220 and 221: Lameness results in major economic
Page 222 and 223: methods; ANOVA (with binary classif
Page 224 and 225: Initially two radiographs were obta
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Key words: calf, active immunity, p
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1 University of Camerino, Veterinar
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vaccinated (Group II) with Pastobov
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# Antigens Association in diarrhea
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1015 Prevalence of Eimeria Infectio
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group showed higher serum lactate c
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thymus in calves and heifers with c
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concentrations of calves with umbil
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The goal of this paper is to expose
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187 Cooling Strategies to Improve M
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inspections, carried out by specifi
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The origin of Hungarian Grey cattle
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University of Warmia and Mazury in
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POSTER ABSTRACTS 1225 The Effect of
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multiple fracture (n=1, 1.3%). The
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position. A fusiform skin incision
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Conclusions: It is the first presen
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1 Faculty of Veterinary Medicine, A
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of 101.55±4.94 mmHg. Afterwards th
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In Mexico, there is a wide variety
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Cardiovascular disorders are common
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Conclusion: The total contamination
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Material and methods: The trial inc
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detection of M. wenyonii infection.
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390 Study on Attenuation Mechanisms
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POSTER ABSTRACTS 1152 Detection of
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digestion. It works only on Apicomp
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determined. In addition the surface
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Africa and Europe, which causes mor
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Results: On D0, the animals include
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addition of mineral salt, during 12
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digestion could be improved with im
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Ltd., Blandford Forum, UK). The air
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cattle ocular squamous cell carcino
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pharmacokinetic/pharmacodynamic (PK
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Bovine Immunodeficiency Virus (BIV)
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significantly lower in groups vacci
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5.00+0.67 and 4.94+0.15 mEq/l in th
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potable water and food, avoiding st
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equal distributions at 08:00 and 16
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electrochemical properties of the a
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Selenium was discovered as an essen
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growth by measuring BW at 15-day in
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influence of different risk factors
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performances of primiparous cows ar
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anaerobes. Aerobic bacteria isolate
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P4 12 to 24 hours before calving is
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exists about Mb prevalence in dairy
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frequency of animals with BoHV anti
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esults, supported by a decrease in
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international drivers of change. Th
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The performance of two groups of no
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spasm is accompanied by kyphosis, w
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occurrence of a crisis of stiffness
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Notices 336 XXV. Jubilee World Buia
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