Oral and Poster Abstracts

potable water and food, avoiding stress, Chapter 7 obligations with
society. To compete version of the ethics code consultation you can
go to www.ammveb.net
Key words: bioethic
1070 Pollution et Syndrome des Corps Étrangers
M. Tlidjane, Z. Rouabah, N. Alloui, B. Safsaf
Batna University, Department of Veterinary Medicine, Algeria
This study has been archived in three areas of the wilaya of Batna.
The first one is located near a great domestic waste discharge at open
sky (Azab).The second area consisted of medium waste discharge
(El-Madher) and the third one without a fixed public waste discharge
(Sériana). Three types of investigation were performed. The first one
concerned the degree of pollution of pasture fields. It showed that
pollution is very high in the immediate nearby of the discharge and
this pollution had a direct effect on the animal health. The second
survey at slaughter-house showed that animals that had ingested the
most non-biodegradable foreign bodies come from breedings located
near great or average waste discharge. The survey on the frequency
of the foreign body syndrome showed a clear relation-ship between
the frequency and the degree of pasture field pollution. This finding
reveals that non-protected waste discharges constitute the main
source of pasture fields pollution and have a direct impact on animal
health.
1071 Genetic Relationships between Linear Type Traits, Somatic
Cell Score and Longevity in Holstein Cows of Iran
M. Bakhtiarizadeh, M. Moradi Shahr Babak, A. Pakdel,
M. Motlagh Khodaie
University of Tehran, Faculty of Agronomy and Animal Science,
Karaj, Iran
The objective of the present study was to estimate the genetic
parameter for 13 linear type traits, somatic cell count and longevity
in Holstein population of Iran.Two set data including 3000 (for SCS)
and 12226 (for longevity) first lactation records on cows distributed
across 219 and 1500 herd-year-season groups respectively. SAS
software and GLM proc were used to fit the model. Genetic
parameters were estimated by restricted maximum likelihood with
an animal model and ASREML software. Heritability estimates for
the type traits varied from 0.033 (foot angle) to 0.29 (stature).genetic
correlation among type traits ranged from 0.01 (suspensory ligament
and Body Depth) to 0.83 (stature and rump length). Genetic
correlation among type traits and longevity (Functional Length
Productive Life) ranged from 0.41 (udder depth) to -0.5 (chest with)
and among type traits and SCS ranged from 0.85(rear udder width)
to -0.6 (fore udder attachment). Most of the genetic correlations
among type traits (except foot angle, fore udder attachment, rear
udder height, suspensory ligament and fore teat placement) with
longevity and SCS had a reciprocal sign or in other words cows with
more longevity had a lower SCS and cows with lower longevity had
a more SCS. In result more attention should pay to these traits in
breeding programs for better animal welfare and profit.Negative
genetic correlation among foot angle, udder depth, fore udder
attachment and rear leg side view with SCS and also positive genetic
correlation among suspensory ligament, udder depth, fore teat
placement and rear leg side view with longevity indicate that these
traits can be used within a selection index in national progenytesting
program. In general results of this study indicated that
genetically taller, wider, deeper with bigger rump length cows have
a more SCS and lower longevity.
Key words: type traits, SCS, longevity
1072 Effect of Cadmium Ion on Peroxidase Activity of Serum in
Cow: (An in vitro study)
H. Tayefi-Nasrabadi 1 , Gh. Moghaddam 2 , Z. Rajabi 3 , Gh. Dehghan 4
1 Faculty of Veterinary Medicine, University of Tabriz, Basic
Sciences, Tabriz, Iran
2 Faculty of Agriculture, University of Tabriz, Animal Sciences,
Tabriz, Iran
3
Faculty of Veterinary Medicine, University of Tabriz, Clinical
Sciences, Tabriz, Iran
4
University of Tabriz, Faculty of Veterinary Medicine, Tabriz, Iran
304 XXV. Jubilee World Buiatrics Congress 2008
Cadmium is a widely distributed metallic pollutant of our
environment, which can be absorbed into biological systems through
direct uptake as well as by accumulation in food chains. It has toxic
effects on all living systems, whether human, animal, plant or
bacterium. Peroxidases are important detoxifying enzymes system
serving to rid cells of excess H 2O 2 under normal and stress
conditions, including contamination by toxic levels of heavy metals.
However, extreme stress conditions may affect the activity of the
detoxification enzymes themselves. For clarifying some possible
mechanism of cadmium toxicity, the effect of increasing amounts of
Cd 2+ ion on peroxidase activity was investigated in vitro in serum of
cow. The H 2O 2-mediated oxidation of o-dianisidine was used to
assess the peroxidase activity. Results show that after preincubation
of serum with 0.25-100 mM Cd 2+ concentration for 5 minutes,
peroxidase activity was inhibited compared to the control and
decreased rapidly with increasing metal concentrations. The enzyme
was completely inhibited after 5 min preincubation in 100 mM Cd 2+ .
When the preincubation of serum and Cd 2+ was prolonged to 0.5, 1
and 24 hours, the enzymatic activity decreased more rapidly with
increasing metal concentration, and the enzyme was completely
inhibited at lower metal concentrations (at 50 mM Cd 2+ after 30-min
preincubation, at 30 mM Cd 2+ after 60-min preincubation and at 5
mM Cd 2+ after 24-h). By considering of long biological half-life of
cadmium in body of animals, it suggested that the damage caused by
exposure to heavy metals is often not only dose-dependent, but also
time-dependent. Even though detoxifying enzymes may not show
any effect after brief exposure to low concentrations of heavy
metals, prolonged incubation will affect the enzymatic activity,
leading eventually to complete inactivation.
Key words: cadmium, time dependent, peroxidase activity, serum,
cow
1073 In situ Dry Matter Degradation Kinetics of Treated and
Untreated Sainfoin (Onobrychis Viciifolia)
H. Khalilvandi, K. RezaYazdi, M. Dehghan-Banadaky,
N. Vahdani, H. Khazanehei
University of Tehran, Animal Science Department, Karaj, Tehran,
Iran
Sainfoin is tanniferous legume forage with CT concentration of 2.5
to 7.7 % of dry matter. In order to investigation of different
chemicals to improving degradation characteristics of Sainfoin, an In
situ Experiment carried out using 3 ruminally fistulated Holstein
cows. Samples of forage were chapped 3-5 cm length, and
then treated with NaoH (0.05 M) and Sodium Bicarbonate (0.1 M)
solutions with forage to reagent volume ratio of 1:4 (W/V).
Treatments carried out in 25 ° C temperature, for 20 min, with hand
shaking. 5 % solution of PEG (6000 MW) was sprayed to forage
with 1:1 ratio of 1:4 (W/V). Treated forages then expose to 40 ° C
temperature in a forced air oven, for 48 hour. All forage samples
grind to pass 2 mm screen size (Wiley mill). 5 g of forage samples
were weighed into nylon bags (10_20 cm) with 53 µm pore size.
Duplicates were incubating for 4, 8, 12, 24, 48, 72 and 96h in ventral
rumen. PEG treated forage has greater rapidly soluble fraction (a),
PEG treatment can not increase potentially degradable fraction (b),
but NaoH solution was done. Effective degradability in different
rumen dilution rates were high for PEG treated Sainfoin compared to
others.
Treatment a (%) b(%) C(h -1 ) K=0.03 K=0.05 K=0.08
(h -1 ) (h -1 ) (h -1 )
PEG 47.7 a 32.8 ab 0.076 a 69.03 a 64.53 a 60.06 a
NaHCO 3 37.4 b 30.17 b 0.076 a 56.53 c 52.26 c 48.06 c
NaoH 35.9 c 37.0 a 0.08 a 61.2b 56.5 b 51.73 b
Control 33.4 d 36.35 a 0.066 a 57.2b c 54b c 49.9b c
Treatment Rumen incubation time(h)
4 8 12 24 48 72 96
Control 40.71 b 49.37 b 51.52 b 62 c 67.43 b 69.25 bc 70.22 bc
NaHCO 3 39.03 b 43.8 c 50.06 b 62.92 c 66.4 b 67.04 c 67.13 c
NaoH 42.64 b 50.13 b 53.94 b 68.86 b 70.6 b 72.50 b 73.08 b
PEG 50.36 a 56.88 a 62.16 a 75.56 a 79.34 a 80.01 a 80.15 a