Oral and Poster Abstracts

clinical examination did not show other congenital abnormalities
such as hair lip and Clift palate.
Conclusions: In a case we can find more than one congenital
abnormalities such as case former, also although some congenital
abnormalities not-life threatening, is usually considered an important
defect if detected during soundness or respiratory examination such as
second case.
Key words: congenital abnormalities, tail absence, spina bifida
occulta, achondroplasia, brachygnathia, calves
998 Diarrhea of Neonatal Calves due to Klebsiella (First Report
from Iran)
F. Zavoshti 1 , M. Dezfouli 2 , A. Bahonar 3 , M. Rabbani 4 , T. Salehi 4
1 Faculty of Veterinary Medicine, University of Tabriz, Department of
Clinical Sciences, Tabriz, Iran
2 Faculty of Veterinary Medicine, University of Tehran, Department
of Clinical Sciences, Tehran, Iran
3 Faculty of Veterinary Medicine, University of Tehran, Department
of Food Hygiene, Tehran, Iran
4 Faculty of Veterinary Medicine, University of Tehran, Department
of Microbiology, Tehran, Iran
Objectives of the study: Clinical evaluation of Klebsiella induced
diarrhea and frequency of this agent in the diarrhea of calves in Iran.
Materials and Methods: Procedure was collecting stool sample
from rectum of the all affected and apparently healthy calves in a
cross sectional study and using bacteriological standard methods for
detection of this bacterium. Statistical analysis was SPSS package.
Results: 31 samples out of 209 diarrheic calves (lees than one month
age) was examined stool examination that was infected with
Klebsiella. In this regard, 18 samples (8.6%) were from diarrheic
calves and 13 samples (15.3%) from control group. Mean of age in
diarrheic calves was 12.00±3.08 days. In diarrheic group 6 calves
(33.3%) showed severe diarrhea. All diarrheic calves (100%)
showed thin and vivacity in general condition. Nine diarrheic calves
(50%) had yellow feces. 17 of diarrheic calves (94.4%) had poor
feeding behavior and all diarrheic calves (100%) showed second
dehydration degree.
Conclusions: The clinical implication is Klebsiella can be one of
causative agents in the calves’ diarrhea syndrome in the less than one
month age.
Key words: Klebsiella, diarrhea, calf, Iran
999 Clinical Trial of Different Type of Dried Colostrum Powders
in Neonatal Holstein Calves to Prevent Diarrhea in Iran
F. Zavoshti 1 , M. Dezfouli 2 , M. Rabbani 3 , T. Salehi 3 , H. Seifi 4
1
Faculty of Veterinary Medicine, University of Tabriz, Department of
Clinical Sciences, Tabriz, Iran
2
Faculty of Veterinary Medicine, University of Tehran, Department
of Clinical Sciences, Tehran, Iran
3
Faculty of Veterinary Medicine, University of Tehran, Department
of Microbiology, Tehran, Iran
4
Ferdowsi University of Mashhad, Department of Clinical Sciences,
Mashhad, Iran
Objectives of the study: Transfer of passive immunity in newborn
calves occurs through the oral consumption and subsequent
absorption of immunoglobulin (Ig) soon after birth. Prevalence of
failure of passive transfer (FPT) in calves has been reported to range
from 11 to 31% in North America. Many attempts have been made to
artificially augment the ability of calves to attain passive immune
support, such as stored surplus colostrum, injectable Ig solutions,
dried colostrum and concentrated milk whey. However, none of
these methods have proven to provide adequate passive immune
support. The aim of this study was to test the value of lyophilized
and spray-dried colostrum as a colostrum supplement on the
incidence of neonatal calf diarrhea.
Materials and Methods: One hundred and twenty healthy Holstein
calves were assigned randomly at birth to one of four groups; each
group contained 30 calves and the calves were received as follows:
group A, 85± 5 g of lyophilized colostrum powder dissolved in 3 kg
of whole milk; group B, 85± 5 g of spray- dried colostrum powder
dissolved in 3 kg of whole milk; group C, 750 ml frozen and thawed
colostrum in 3 kg of whole milk, and group D, only 3 kg of whole
milk without colostrum supplement. Each group was subdivided into
3 sub- groups of 10 calves as follows: calves fed colostrum
supplement at 24 or 48 or 72 h after birth. All calves fed fresh
colostum within 6 h after birth at the amount of 5% BW by bottle.
Results: Calves which were fed lyophilized colostrum supplement had
a significant lower incidence of diarrhea in comparison to control
calves. In addition, calves were received lyophilized colostrum
supplement at 24 h after birth showed less incidence of diarrhea than
calves fed supplement at 48 and 72.
Conclusions: According to the results of present study providing
lyophilized colostrum supplement at 24 h after birth might have been
preventive effect on calf diarrhea. The spray- dried colostrum
supplement had not the same effect.
Key words: calf, diarrhea, colostrum, lyophilized, spray-dried
1000 The Importance of Acute Phase Protein Measurements in
the Clinical Diagnosis of Calves Experimentally Induced
with Lipopolisaccharide (E. coli) Endotoxemia
A. Coskun, I. Sen
University of Selcuk, Faculty of Vet. Med., Department of Internal
Medicine, Konya, Turkey
The objectives of this study was to determine the clinical changes,
the importance of disseminant intravasculer coagulopathy and
concentrations of acute phase proteins (Haptoglobin, fibrinogen and
Serum Amilod A) in the diagnosis and prognosis of calves that were
experimentally induced with Lipopolisaccharide (LPS) E. coli
endotoxemia.
Materials and methods: a total of eight healthy Holstein calves,
aging 30 to 45 days old were used in this study. In all calves, the
endotoxemia were induced by giving 0,1Ïg/kg doses of LPS in 50 ml
phisyologic saline via intra-venous route in constant infusion during
30 minutes. Blood samples were collected before (24 hour) and after
the endotoxemia induction at 0, 0.5 th, 1, 2, 4, 6, 8, 12, 18, 24, 48, 72,
96, 120, 144, 168, 192, 216 and 240th hours of study. In addition,
clinical symptoms of endotoxemia were also observed following
LPS infusion.
Results: in general, abnormal clinical changes such as anorexa,
depression, increased respiratory and heart rate were observed 30
minutes later following LPS infusion. Haemotological examination
showed that a significant leucopenia, folowed by a leucousytosis and
decresead T and B-Lyphocytes levels were detected at the first hours of
study. Abnormal chances were also observed in cougulation
parameters such as APTT, PT, trombosit and fibrinogen during
study.The plasma Haptoglobin (Hp) concentrations were increased
from basal levels (8,13 µg/ml) to 20,84µg/ml levels at 6 th hours
following LPS infusion. The highest Hp concentration (500,08 µg/ml)
was found at 36 th hours after LPS infusion. And than, the Hp levels
were declined to basal levels at 144 th hour of study. First 3 hours
following LPS infusion, the Serum Amilod A (SAA) concentrations
have increased from basal levels (12,04 µg/ml) to 30,22 µg/ml levels,
and the highest concentration (185,27 µg/ml) of SAA was measured at
24. at hours of study. And than SAA level has declined to basal levels
at 120 th hours of study.
Conclusion: based on clinical and laboratory findings, the
endotoxemia was successfully induced by giving 0,1µg/kg doses LPS
via intra-venous route in constant infusion. Results suggest that both
Hp and SAA could be used as a indicator in the diagnosis of calves with
endotoxemia. But, we could be say that haptoglobin might be a better
prognostic indicator for calves with endotoxemia compare to SAA.
This study was supported by both Tubitak and S.U. BAP
Key words: calf, LPS, haptoglobin, SAA, endotoxemia
1001 Protective Effect of Pastobov and Alterations of Peripheral
Blood Leukocyte Subpopulations in Calves Experimentally
Challenged with Mannheimia haemolytica A1 Leukotoxin
D. Bednarek 1 , R. Urban-Chmiel 2 , K. Dudek 1
1 National Veterinary Research Institute, Department of Cattle and
Sheep Diseases, Pulawy, Poland
2 Faculty of Veterinary Medicine University of Agriculture,
Department of Veterinary Prevention, Institute of Infectious and
Invasive Diseases, Lublin, Poland
The aim of the study was to evaluate the effect of Mannheimia
haemolytica A1 leukotoxin (LKT) on clinical status and alterations
of cellular immune response in calves vaccinated (Group I) and non-
Calf Physiology, Management and Diseases 231