Oral and Poster Abstracts

lesions in other organs. Young animals most frequently become infected
by ingestion of infected milk. We present a case report of tuberculous
meningoencephalitis in a dairy calf that was less than one year old, who
presented nervous signs (recumbency, irritability and aggression,
weakness, dizziness), which progressed to death in three days. At
necropsy, white nodules were found in the liver, lungs and diaphragm.
The clinically suspected diagnosis was rabies, but results were negative
for direct immunofluorescence and intracerebral inoculation in mice.
Brain samples and spinal cord were preserved in 10% formalin for
histopathological analysis (HE staining). There was severe granulomatous
meningomyelitis with Langhans’ giant cells. In cerebral neuropile there
were some granulomas with a central area of necrosis, perivascular
cuffing with mononuclear cells, gliosis and hyperemia. The lung had
many caseated granulomas, a typical finding in bovine tuberculosis, with
central necrosis, surrounded by epithelioid macrophages, lymphocytes,
plasma cells, Langhans’ giant cells and fibrosis at the periphery. There
was mild calcification of the pulmonary granulomas. Samples of lung and
brain were decontaminated by the Petroff method and inoculated into
Petragnani and Stonebrink media and M. bovis was isolated. This case
report shows that bovine tuberculosis should be included in the
differential diagnosis of bovine neurological disorders. According to
Brazilian regulation, sanitary measures in the heard infected (test-andslaughter)
should be adopted.
FAPESP
Key words: tuberculosis, Mycobacterium bovis, meningoencephalitis,
calf
712 A Study on Diagnosis, Pathogenesis of Pseudomonas
aeruginosa Isolated from Different Cases of Human and
Animals in Mosul City - Iraq
D. Ali 1 , B. Ismaeel 2
1 College of Veterinary Medicine, University of Mosul, Iraq,
Department of Microbiology, Mosul, Iraq
2 College of Veterinary Medicine, University of Dohuk, Department of
Microbiology, Duhok, Iraq
The current study tackles the isolation and identification of
Pseudomonas aeruginosa from different human infection cases
(wounds, otitis media, urine, stool, and burns) in Al-Zahrawi, Al-Salam
and Ibn-Al-Atheer hospitals at Mosul city. From animals (sheep, cows,
goats) samples were also collected (wounds, otitis, urine, feces and
mastitis) cases for P. aeruginosa isolation and identification. The
morphological, cultural and biochemical tests confirmed 43 (17.4%)
isolates as P. aeruginosa from 250 human samples, and 17 (10.3%)
from 165 animal samples. P. aeruginosa human and animals’ isolates
virulence factors represented by enzymes (protease, lipease,
lecithinase, b-lactamase gelatinase, coagulase, haemolysin, toxins) and
capsule production were studied; 80% of human isolates showed
capsule presence, while all animal isolates (100%) contained capsule.
The pigments production especially pyocyanin, which differentiates P.
aeruginosa from other microorganisms, was detected in 80% of the
human isolates and in 100% of the animal isolates. The study showed
similarity between P. aeruginosa of human and animal isolates in their
virulence factors production ability. The study results revealed that 15
pathogenic isolates from 43 tested human isolates and 10 pathogenic
isolates from 17 tested animal isolates produced positive results for an
exotoxin A production in rabbit skin reaction test, through inducing
hypersensitivity reactions of type I (early or immediate) and of delayed
type (Type IV). Baby suckling mice test and rabbit ileal loop test
confirmed that both human and animal P. aeruginosa isolates produced
an enterotoxin. The histopathological study revealed different changes
represented by elongation and blunted villi with necrosis.
713 Bovine Paralityc Rabies: Prevention and Control in Mexico
M. Manzano 1,2 , S. Vázquez-Morón 2 , R. Ramirez 1 , M. Orozco 3 ,
J. Echevarría 2 , A. De Vega 1
1
Comite de Fomento y Salud Animal, Campana de Rabia, Puebla,
México, Mexico
2
Instituto de Salud Carlos III, Servicio de Microbiologia
Diagnostica, Madrid, Spain
3
Unión Ganadera Regional Sierra Oriente, Gerencia, Teziutlan,
Mexico
Bovine paralytic rabies is a contagious and fatal disease that affects a large
number of productive animal species and also humans, creating a risk both
to human and animal health. In America this involves a serious problem.
One of the determining factors to prevent cases of rabies in animals and in
humans is, without any doubt, the diffusion and training on measures of
prevention and control of the disease. A DVD on this kind of measures
was recorded in the City of Puebla, Mexico, by the Committee of
Promotion and Animal Health and the Foundation “PRODUCE”, Puebla.
First, the DVD has been presented all over the country, and, on a
subsequent occasion, it was presented in the RITA (The international
meeting on research advances in rabies control in the Americas) meetings
of Brazil and Mexico, in coordination with the Panamerican Health
Organization. The DVD was distributed to all American countries
through these meetings.The mentioned DVD shows two aspects of this
problem: in the first part a case of an outbreak of paralytic rabies is
narrated in the colloquial language of the cattle farmers from the northern
mountains belonging to the Puebla State; while the second part includes
recommendations about the cold chain, antirabies vaccination, sending
specimens to the laboratory, classification of bats and control of
hematophagous bats.Furthermore, molecular studies are currently being
realized in the Instituto de Salud Carlos III (Madrid, Spain) to
complement the field work made in Mexico. For all the reasons exposed it
is of great interest to share this experience with European Buiatric groups.
714 Effect of Cow Vaccination against BRSV and PI3 on Immune
Status ante Partum and the Transfer of Colostral Antibodies
to Calves
B. Makoschey 1 , R. Brunner 2 , M. Koenig 3 , C. Gumpert 4 , H. Heckert 4
1
Intervet Schering-Plough, Int. Marketing, Boxmeer, The Netherlands
2
Intervet Schering-Plough, Businessunit Cattle, Unterschleissheim,
Germany
3
Justus-Liebig-Universität Gießen, Faculty of Veterinary Medicine,
Institute of Virology, Giessen, Germany
4
Freie Universität Berlin, Clinic for Ruminants and Pigs, Berlin,
Germany
Objectives: The effect of immunization of pregnant cows with
respiratory pathogens (Bovine Respiratory Syncytial Virus (BRSV) and
Parainfluenza Type 3 (PI3)) on the antibody levels in the serum and
colostrum as well the transfer of the colostral antibodies to the newborn
calves should be determined.
Material and Methods: The study was performed on a dairy farm where
the dams and their calves are housed at two different locations. A
commercial vaccine containing inactivated antigens of BRSV, PI3 and
Mannheimia haemolytica (M. haemolytica) (Bovipast ® RSP / Bovigrip
RSP plus, Intervet) was used. Twenty-six dams (n=26) were vaccinated
twice (6-8 and 2-4 weeks before the anticipated date of calving). A group
of 19 cows was not vaccinated with the respiratory vaccine. In addition,
all cows (n=45) were routinely immunized with a neonatal calf diarrhea
containing inactivated bovine rotavirus, bovine coronavirus and E. coli at
6 weeks before calving. Colostrum was collected from each cow and fed
to the respective calf at a volume of at least 4 liter within the first 12 hours
of life. Blood samples of the dams were taken at the time of the first
vaccination and around calving. Moreover, samples of the first milkings
were collected. Blood samples of the calves were taken two days after
birth. Neutralising antibody titers against BRSV and PI3 were measured
in the serum and colostrum samples.
Results: The interpretation of results regarding BRSV was troubled by
high pre-vaccination antibody levels in a certain proportion of animals.
Significantly higher antibody titres against BRSV were found in dams
from the vaccinate group after adjustment of measured titres by
subtracting individual pre-vaccination values. Moreover, there was a
correlation between the BRSV antibody titer in the cows and the titers
measured in the calves. With regard to PI3, vaccination of dams resulted
in a booster of neutralizing antibodies and a transfer of the antibodies to
their newborn calves via colostrum regardless the pre-vaccination titer
against PI3. Moreover, the PI3 antibody titers in the cows correlated well
with the titers measured in the two days old newborns.
Conclusions: Cow vaccination against BRSV and PI3 could be a
strategy to achieve more homogeneous antibody levels in the serum of
the dams and their calves and can therefore complement the active
immunisation program in the calves.
715 Detection of Prevalence of Rotaviral and Corona Viral
Diarrhea in Newborn Calves
B. Shojaee 1 , F. Hemmatzadeh 1 , H. Momtaz 1 , M. Ahmadi bozorg 1 ,
B. Khoramian 2 , M. Eslampour 2
Infectious and Zoonotic Deseases (Public Health) 105