Oral and Poster Abstracts

654 Complete Protection from Renal Infection with Leptospira
Borgpetersenii Serovar Hardjo in Calves after Transfer of
Specific Antibodies
E. Klaasen 1 , T. Mols 1 , M. Smit 1 , B. Makoschey 2
1
Intervet-Schering-Plough, R&D, Boxmeer, The Netherlands
2
Intervet-Schering-Plough, Int. Marketing, Boxmeer, The
Netherlands
Objectives: The purpose of this study was to examine whether
administration of serum antibodies can protect cattle from renal
infection with Leptospira borgpetersenii serovar Hardjo, even if
challenge is performed as long as 7 weeks after serum transfusion.
Materials and Methods: Ten conventional 5-week-old calves free of
serum antibodies against serovar Hardjo were randomly assigned to
two groups of five animals each. Each calf of group 1 received a
transfusion of a hyper-immune serum with a titer of 9.1 [log2/ml] of
antibodies specific for serovar Hardjo. The calves of group 2 (control
group) were left untreated. Seven weeks later all animals were
challenged on three consecutive days by conjunctival instillation of
Leptospira borgpetersenii serovar Hardjo genotype hardjobovis.
Serum samples were taken from the calves of groups 1 and 2
immediately prior to the transfusion as well as 24 hours, 4 weeks and 7
weeks thereafter. Antibodies specific for serovar Hardjo, were
measured by ELISA. Urine samples were taken pre-challenge and 2, 3,
4, 5 and 6 weeks post-challenge to assess the effect of the challenge.
The calves were sacrified 6 weeks post-challenge and kidney tissue was
taken. Urine samples and kidney tissue homogenates were cultured.
The cultures were examined every 3 weeks with dark-field microscopy.
Cultures were considered negative when no leptospires were detected
after 18 weeks of incubation.
Results: The titration of serum samples taken before the transfusion
confirmed that the calves of both groups were free of serum antibodies
against serovar Hardjo and the untreated animals in group 2 remained
seronegative until the time of challenge. By contrast, mean titers of 5.8,
4.0, and < 3.3 were measured in the samples taken from animals of
group 1 at 24 hours, 4 weeks and 7 weeks after transfusion respectively.
Renal infection was demonstrated in four out of five control animals,
whereas in none of the five pre-treated calves renal infection could be
detected.
Conclusion: In this study, complete protection of cattle from renal
infection with serovar Hardjo was obtained with merely humoral
immunity through passive immunization. In this study, the protection
lasted for at least 7 weeks after transfusion. At this time point, the
serum titre of antibodies had declined below the detection limit of the
ELISA.
655 Compatibility of a Live IBR Marker Vaccine and an
Inactivated BVDV Vaccine
B. Makoschey 1 , J. Munoz Bielsa 1 , L. Santos 2 , M. Alvarez 3
1
Intervet-Schering-Plough, Int. Marketing, Boxmeer, The
Netherlands
2
Intervet-Schering-Plough, Laboratorios Intervet S.A., Salamanca,
Spain
3
University of Leon, Animal Health Department, Leon, Spain
Objectives: The target animals and vaccination regimes for vaccines
against the bovine rhinotracheitis (IBR) and the bovine viral diarrhea
virus (BVDV) are very similar. In order to simplify animal handling it
would be desirable to combine the two vaccination procedures.
Therefore, we have examined whether a live IBR marker vaccine
(Bovilis ® IBR marker live, Intervet) and an inactivated BVD vaccine
(Bovilis ® BVD, Intervet) can be applied at the same time.
Materials and Methods: A total of 57 head of cattle were included in
the study and seven groups of animals were established. The animals
were vaccinated on days 0, 28 and 208 according to different
(combined) vaccination schedules. The immune responses against the
bovine herpesvirus type 1 (BoHV-1) and BVDV were determined and
compared for the different vaccination schedules.
Results: BoHV-1 antibody titers after simultaneous (together in the
same syringe) or concurrent (two separate injections) application of the
inactivated BVD vaccine were not lower than after vaccination with the
BoHV-1 vaccine alone. To the contrary, BoHV-1 titers were even
higher after simultaneous application at the first or second dose of the
basic vaccination course of the BVD vaccine and at the booster
vaccination. Likewise, BVDV antibody titers after simultaneous or
concurrent application of the live BoHV-1 vaccine at the first or second
dose of the basic vaccination course of the BVD vaccine and at the
booster vaccination were not different from the titers after vaccination
only with the BVD vaccine.
Conclusions: There was no negative effect on the antibody response
against either of the two vaccines when the live IBR marker vaccine
was applied simultaneously (together in the same syringe) or
concurrently (two separate injections) with the first or second dose of
the basic vaccination course of the BVD vaccine and then at the booster
vaccination (3rd dose).
656 Compatibility of an Inactivated IBR Marker Vaccine and an
Inactivated BVDV Vaccine
B. Makoschey 1 , J. Donate 2 , L. Santos 2 , M. Alvarez 3
1
Intervet-Schering-Plough, Int. Marketing, Boxmeer, The
Netherlands
2
Intervet-Schering-Plough, Laboratorios Intervet S.A., Salamanca,
Spain
3
University of Leon, Animal Health Department, Leon, Spain
Objectives: The target animals and vaccination regimes for infectious
bovine rhinotracheitis (IBR) and the bovine viral diarrhoea virus (BVDV)
vaccines are very similar. To simplify animal handling on the farm it
would be desirable to combine both vaccination regimes. We have
performed a study to assess the efficacy in terms of antibody response of
the simultaneous (both vaccines in the same syringe) and concurrent (both
vaccines at the same time, but different injection sites) use of Bovilis ®
BVD (Intervet, The Netherlands) and Bovilis ® IBR marker inac (Intervet,
The Netherlands) in dairy cattle under field conditions.
Materials and Methods: The study was carried out as a randomised,
partially blinded and controlled field trial on a commercial dairy farm
in Spain. All animals included in the study were shown to be free of
antibodies against the IBR and the BVD virus prior to the first
vaccination. Forty animals were included in the study and assigned at
random to one of the four groups of ten animals each. The vaccination
protocols were as follows: group A received only inactivated IBR
marker vaccine, group B received only BVD vaccine, group C were
vaccinated simultaneously (in the same syringe) with both vaccines,
group D were vaccinated concurrently (both vaccines at the same time,
but in two separate injection sites) with the two vaccines. All animals
were vaccinated twice (at admission (d0) and four weeks later (d 28).
Results: The neutralizing antibody response against BVDV did not
reveal any differences between the group vaccinated only with the
BVD vaccine and the groups that were vaccinated simultaneously or
concurrently with the IBR marker vaccine. Likewise, the neutralizing
antibody titers against BoHV-1 did not exhibit any negative effect by
the simultaneous or concurrent use of the two products as compared to
the single IBR marker vaccination.
Conclusions: These results indicate that the two vaccines can be
applied at the same time either at the same or at different injection sites
without any negative effect on the neutralising antibody response
against the two vaccine viruses.
657 A Serological Study on Bovine Viral Diarrhea - Mucosal
Disease (BVD-MD) in Kurdistan Province
Sh. Fakur 1 , Fa. Hemmatzadeh 2
1 Faculty of Veterinary Medicine Islamic Azad University Sanandaj,
Large Animal Clinical Sciences, Sanandaj, Iran
2 Facutly of Veterinary Medicine, University of Tehran, Large Animal
Clinical Sciences, Tehran, Iran
Objectives of study: Bovine viral diarrhea disease is one of the viral
diseases in cattle that was reported 10-90 percent in many countries.
The causative RNA virus is a member of the family togaviridae and the
genus pestivirus which is antigenically closely related with the virus
of BD in sheep. Cattle are the most sensitive species to virus and are
considered the principal reservoir of BVD viruses. A large proportion
of BVD infections are subclinical and majority seropositive cattle.
Since the virus has affinity for the lymphoreticular tissue this can result
immonosuppression and abortion, produce calves with congenital
abnormalities and persistant infectious (PI), So diagnostic laboratory
procedures such as virus isolation and serologic methods are required.
Materials and methods: In this study 410 serum samples were tested
by serum neutralization test (SN) by using the NADL strain of BVD
virus.
Infectious and Zoonotic Deseases (Public Health) 89