Oral and Poster Abstracts

virulence factors and virulotypes will be presented. More than 50
virulotypes were observed when the results were concatenated. Strains
belonging to the most two frequent virulotypes (9 and 4%) were
positive for the type 8 capsular antigen and for ClfA/B, FnbA, EbpS,
SdrC, Spa, HlA/B/D/G, SspA and lukD/E/M, but differed by the
collagen adhesin and the intercellular adherence factor. Twenty-five
strains were further compared by the multi-locus sequence analysis
(MLST) based upon 7 housekeeping genes: 12 strains belonged to the
clonal complexes 97 and 133, but the other strains belonged to various
sequence types. Though the clonal complex 133 was related to one the
most frequent virulotype, the clonal complex 97 comprised strains
belonging to different virulotypes. Based on this limited number of
strains tested, the clonal relatedness of S. aureus strains based on
MLST analysis can not be transposed to the classification into
virulotypes. Results of similar studies in other countries will help to
choose the immunogens to incorporate into the vaccines.
Key words: Mastitis, cattle, S. aureus, virulotyping, Multilocus
Sequence Analysis
576 The Effect of Fermentated-herb Extract on Subclinical
Mastitis in Dairy Cows
TY. Hur 1 , SJ. Kang 1 , YH. Jung 1 , YJ. Do 1 , CY. Choi 1 , IS. Ryu 1 ,
HS. Kim 1 , YS. Lee 1 , SJ. Park 1 , GH. Suh 2
1 National Institue of Animal Science, Animal Resources
Development, Cheonan, South Korea
2 Chonnam National University, College of Veterinary Medicine,
Gwangju, South Korea
Therapeutical potential of fermentated-herb extract (FHE) in bovine
subclinical mastitis was investigated. The fermentated-herb extract was
made by the process of aqueous extract of twelve herbs inoculated with
yeast and probiotic to provide feed additive. Somatic cell count (SCC),
milk yields and total bacterial count (TBC) were evaluated after fifty
grams of the FHE fed each cows per day for 4 weeks. The results
revealed that the FHE reduced the TBC and SCC, and increased milk
yields. The results suggest that the components of FHE possesses some
biologically activity in bovine subclinical mastitis. As such, the present
work substantiates the therapeutic use of fermentated-herb extract and
also emphasizes on the potential of the commonly available non-toxic
substance to enhance the cow health. However, more experiments are
necessary for confirmation of these results.
Key words: mastitis, fermentated herb, somatic cell count
577 Prognosticable Fatal Outcomes of Naturally Occurring
Escherichia coli Mastitis in Dairy Cows – Consumption
Coagulopathy, Hemoconcentration and Hepatobiliary
Damage
K. Mori 1 , S. Hagiwara 2 , H. Okada 3 , H. Nagahata 4
1
Engaruchiku NOSAI, Clinic of Farm Animals, Engaru, Japan
2
Hokkaido NOSAI, Livestock Insurance, Sapporo, Japan
3 National Institute of Animal Health, Research Team for Prion
Diseases, Tsukuba, Japan
4
Rakuno Gakuen University, Department of Animal Health, Ebetsu,
Japan
Objectives: To determine diagnostic characteristics capable of
prognosticating the fatal outcomes of naturally occurring Escherichia
coli mastitis in dairy cows.
Materials and Methods: Vital signs (temperature, pulse, respiration)
were measured, and blood tests, including white blood cell (WBC)
count, antithrombin (AT) activity, platelet (Plt) count, hematocrit (Ht)
value,-glutamyl transpeptidase (GGT) activity and non-esterified fatty
acid (NEFA) levels, were conducted for all the cows. These values
were compared between 7 cows that died due to E. coli mastitis and 17
cows that survived. Five blood test parameters (AT, Plt, Ht, GGT and
NEFA) that showed significant differences between the dead and
surviving cows were used to determine the cut-off values; these
parameters would also be used as criteria to differentiate between the
outcomes. The diagnostic criterion to prognosticate the fatal outcome
was decided using the cut-off values. The practicability of the
diagnostic criterion was verified by trial use on 51 other clinical cases
(12 dead and 39 surviving cows).
Results: All the values measured within 12 hours of onset (day 1) did
not vary between the dead cows and those that survived. However,
significant differences in the five parameters (AT, Plt, Ht, GGT and
NEFA) were observed from the second day (day 2). The diagnostic
criterion for fatal prognosis was determined using the cut-off values of
the above-mentioned parameters. The fatal outcomes could be
predicted if at least two of these parameters were within the ranges
defined by their cut-off values (AT < 120%, Plt < 15 x 10 4 /ml, Ht 32%,
GGT 45 IU/l and NEFA > 0.6 mEq/l). The sensitivity, specificity,
predictive value and likelihood ratio of the diagnostic criterion on day
2 were 100%, 76%, 64% and 4.25, respectively. Verification of the
practicability of the diagnostic criterion in the 51 other clinical cases
examined on day 2 showed the sensitivity, specificity, predictive value
and likelihood ratio to be 67%, 69%, 40% and 2.16, respectively.
Conclusions: Consumption coagulopathy (low AT and Plt),
hemoconcentration (high Ht) and hepatobiliary damage (high GGT and
NEFA) were determined as the diagnostic characteristics in the fatal
cases. AT, Plt, Ht, GGT and NEFA values can be used as criteria for the
prognosis of fatal E. coli mastitis.
578 Pseudomonas Mastitis: Elimination from Contaminated
Wash-water Systems by Slightly Acidic Electrolyzed Water
K. Kawai 1 , M. Kitou 1 , T. Mitamura 2 , I. Uchida 3 , K. Hirose 4 ,
H. Nagahata 5
1
Seibu Veterinary Clinical Center, Tokachi NOSAI, Shimizu, Japan
2
Ecolofrontier Co., Ltd., Sapporo, Japan
3
Hokkaido Research Station, National Institute of Animal Health,
Sapporo, Japan
4
Clinical Research Center, MeiJi Seika Kaisha Ltd., Yokohama,
Japan
5
School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu,
Japan
Objectives of study: Pseudomonas aeruginosa (PA) is usually
responsible for less than 1% of the mastitis infections in dairy cows.
The source of infection for PA mastitis is contaminated water used to
wash the udder or when antibiotics used for intramammary infusion
have become contaminated. PA mastitis infrequently causes clinical
mastitis and their spread of infection inflicts damage on the farms. In
this study, we present effective mastitis control in dairy herd with
increased incidence of PA mastitis.
Materials and Methods: 387 dairy cows were milked in the parlor.
The preventing measures of PA mastitis were started since Oct. 2005.
Milk samples of clinical cases were obtained for bacteriologic culture,
and isolated bacteria were identified using standard techniques. Liners,
jeters, drop hoses and wall of cistern in the parlor were wiped with
cotton swabs and cultured. Milking procedure was checked and
changed to the recommended procedure. The chlorine concentration of
water was increased from 0.05 ppm to 0.5 ppm. Milking equipment and
floor of parlor were sterilized by 200 ppm sodium hypochlorite. The
cows infected with PA were isolated and milked at the end. The milk
samples of all cows were cultured after calving and before dry period.
Slightly acidic electrolyzed water system (Purester MP240E: Morinaga
Milk Industry Co., Ltd., Tokyo) was introduced to the parlor and was
used at milking time since Jan. 2006.
Results: The occurrences of PA clinical mastitis were 35cows in 2003
and 45cows in 2004. After implementation of the preventing measures,
the occurrence of PA clinical cases was 8.3% on all clinical mastitis in
2005. P. aeruginasa, P. fluorescences and P. putida were isolated from
environment (eg, liners, jeters, drop hoses, wall of cistern in the parlor
and reservoir in free stall), and same pathogens were isolated from
clinical cases and 23 of nonclinical cases. However, after the use of
slightly acidic electrolyzed water (hypochlorous acid: available
chlorine, 12 ppm) in 2006, Pseudomonas sp. in the environment, the
occurrence of PA clinical mastitis and the reservoir of Pseudomonas
sp. in the nonclinical cases were disappeared.
Conclusions: The use of slightly acidic electrolyzed water at milking
time is beneficial in reducing exposure of the cows to the organism and
it was proved to be effective on prevention of PA mastitis.
Key words: mastitis, P. aeruginosa, slightly acidic electrolyzed water
579 Acute Clinically Mastitic Animals in Some Assiut
Governorate Villages: Diagnosis and Treatment
M. Sayed 1 , A. Abd El-Rady 2
1
Faculty of Veterinary Medicine, Assiut University, Food Hygiene,
Assiut, Egypt
2
Faculty of Veterinary Medicine, Assiut University, Animal Medicine,
Assiut, Egypt
Mastitis / Udder Health and Milk Quality 59