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libido in unmotivated males. Similarly, identification of diestrus specific chemicals in urine may be applicable to monitor the cyclicity. The bull specific compounds are immense beneficial in induction of estrus in delayed pubertal or postpartum anestrus female and also to enhance estrus intensity in silent estrus individual. In the present study, urinary volatile chemical compounds were identified in samples from cows at estrus and diestrus stage and from sexually active bulls, using gas chromatography mass spectrometry (GC-MS). Dichloromethane was used for extraction of urine and identification of analytes compound was carried out in Perkin Elmer gas chromatograph linked to mass spectrometry. The analyses of cow and bull urine revealed the presence of total fifteen compounds with molecular weights ranging from 114 to 446 Da. GC profile of estrus cow urine was specified by the presence of two compounds viz., Octane and 3hexanol 2,2-dimethyl, whereas, only one compound (decane, 6ethyl, 2-methyl) was identified from urine of cows at diestrus stage. The chromatographic profile of bull urine was demarcated by the presence of three specific compounds namely, nonacosane; 1,2, benzene dicarboxylic acid- dihexyl ester and heptacosane. The present study is a novel attempt to provide valuable chemical information on the urinary compounds that might have potential role in olfactory communication among the conspecific members. However, behavioural assay with synthetic compounds is indicated to acquire more information on the olfactory communication and to identify the sex or estrus specific marker in this species. Key words: urine, chemo-signal, cow, bull, gas chromatography-mass spectrometry 853 The Effect of Season and Parity on Heat-synch Program in Dairy Cows M. Bolourchi, R. Emadi, A. Niasari-Naslaji, P. Hovareshti Faculty of Veterinary Medicine, University of Tehran, Department of Clinical Sciences, Faculty of Veterinary Medicine, Tehran, Iran This study was conducted to investigate the effect of season and parity on Heat-Synch program in dairy cows. Dairy cows (n=230) from two dairy farms located in the vicinity of Tehran,with the average daily milk of 30 kg and with voluntary waiting period of 50 days were assigned into 2 groups (Control and Heat-Synch) during 4 seasons considering their parity (uniparous and multiparous). All cows received 2 luteolytic doses of prostaglandin F 2a analogue (PGF; 500 µg cloprostenol, Nasr Company, Iran) on Days 35 and 48 after parturition. Cows in the control group (n=148) did not receive any further treatment and artificially inseminated with frozen semen 12 hours after showing standing estrus. Cows in Heat-Synch program (n=82) received an intramuscular injection of 12.5 µg GnRH analogue (Luliberin, Aburaihan, Iran; Day 0) followed by an intramuscular injection of PGF on Day 7. On Day 8, females in these groups received an intramuscular injection of estradiol benzoate (1 mg; Aburaihan, Iran). Estrous detection was performed for 48 hours after estradiol injection. Females, displayed standing estrus, were inseminated with frozen semen. Those females that did not display estrus were inseminated blindly at 48 hours after estradiol injection. Pregnancy was diagnosed on Day 35-40 after AI via rectal palpation. First service conception rates were analyzed using Genmod procedure in SAS including logit statement in the model. First Service conception rates were not different among groups between the two dairy farms. Therefore data from these dairy farms were pooled for further analyses. There was no significant difference between two groups regarding different seasons [Control: spring: 39.53% (17/43); summer: 61.11% (11/18); fall: 57.45% (27/47); winter: 55% (22/40); Heat-Synch: spring: 64% (16/25); summer: 66.67% (6/9); fall: 50% (11/22); winter: 57.69% (15/26); P>0.05]. There was also no significant difference between two groups regarding their parity [Control: uni-parous: 48.6% (18/37); multi-parous: 53.1% (59/111); Heat-Synch: uniparous: 35.7% (5/14); multiparous: 64.2% (43/67); P>0.05]. In conclusion, season and parity did not have any significant effects on first service conception rates in dairy cows enrolled in Heat-Synch program compared to control. 854 Determination of PAG in the Milk and Blood in Cow’s in Comparison to other Examination Methods for Pregnancy Diagnosis Z. Gajewski 1 , N. Melo de Sousa 2 , A. A Konopka 1 , JF. Beckers 2 , R. Thun 3 190 XXV. Jubilee World Buiatrics Congress 2008 1 Warsaw University of Life Sciences, Departament of Animal Reproduction, Warsaw, Poland 2 University of Liege, Department Physiology of Reproduction, Liege, Belgium 3 University of Zurich, Clinic of Reproduction, Zurich, Switzerland Introduction: In clinical practice a few pregnancy diagnostic methods, which differ in effectiveness and time are used, one of the methods is measuring PAG (Pregnancy Associated Glycoprotein) concentration in milk and blood. Aims and methods: the aim of the present study was to use a new developed method for evaluating the PAG in milk and blood and compare these results with USG and rectal investigation for pregnancy diagnosis in the cows. The experiment was carried out on a group of 220 cows of HF breed. The used animals were 3 to 8 years old. All animals underwent pregnancy testing: ultrasonography with an ALOKA SSD-500 with 5.0 MHz probe, 30-55 days after insemination and rectal control after the 55 th day of pregnancy. Blood and milk were used for PAG tests. The concentration of two PAGs, bPAG and cPAG, was measured in milk and in serum the concentration of bPAG was determined. Blood samples were taken from the jugular vein (v. jugularis) and 50 ml milk samples were taken and stored at -20 °C. Results: the profile of bPAG and cPAG concentration in milk increased after 28 day of pregnancy and showed a rapid increase close to parturition. The same results we observed in the blood concentration of bPAG. The drop of bPAG concentration was faster in the milk than in the blood. We found a good correlation between the results from the blood, milk, rectal palpation and USG pregnancy detection. The investigations showed that the concentration bPAG and cPAG in milk were not higher than 10 % of the bPAG concentration in the blood. Analysis of variance presented the correlation between pregnancy and cPAG concentration in milk (P < 0.1), and its higher than with bPAG in the blood plasma, what suggests lower correlation. Conclusion: our data showed that the RIA method was very precise for measuring PAG concentrations in the maternal blood and milk of the investigated cows. The results showed the possibility of the use of cPAG in milk and bPAG and cPAG in blood as pregnancy test to replies the traditional rectal investigation or USG method. Key words: PAG, ultrasonography, cows 855 Efficiency of Different Cloprostenol Doses in the Postpartum Period of Zebu (Bos Taurus Indicus) Beef Cows C. Fernandes 1,2 , B. Alves 2 , E. Oliveira 2 , J. Viana 3 , A. Figueiredo 1,2 , M. Gioso 1 , E. Oba 4 1 University of Alfenas, Veterinary Medicine, Alfenas-MG Brazil, Brazil 2 Biotran, Veterinary Medicine, Alfenas-MG Brazil, Brazil 3 Embrapa Gado de Leite, Animal Reproduction, Juiz de Fora - MG - Brasil, Brazil 4 UNESP- School of Veterinary Medicine and Animal Sciences, Animal Reproduction, Botucatu, Brazil Reestablishment of reproductive activity after parturition depends on two physiological processes, uterine involution and reestablishment of the ovarian luteal cyclic activity. Impaired or delayed uterine involution can also affect ovarian activity. Prostaglandins have an important function in this process. The prostaglandin F 2a (PGF 2a), normally produced during uterine involution, stimulates uterine muscular layer activity. The use of PGF 2a synthetic analogous in bovine postpartum, however, has been limited. Many studies demonstrate a beneficial effect of synthetic prostaglandins in the postpartum period of cows, but it is still not clear whether their isomers would have a similar effect. The kinetic of cloprostenol isomers can be different in the receptors located in the uterus or in the corpus luteum. The aim of this study was to compare the effect of two different doses of a racemic cloprostenol mixture (D+L- Cloprostenol) given in the postpartum period, on reproductive performance of Nelore (Bos taurus indicus) cows. The study was performed in a farm in Minas Gerais State, Brazil. Beef cows with normal parturition were evaluated and randomly distributed into 3 groups: G1 (n=144), Control Group; G2 (n=145), 0.530mg of D+L- Cloprostenol, given IM at 3 to 5 days after parturition, and G3 (n=145), 1.06 mg of D-Cloprostenol, in the same schedule as in group 2. Parameters evaluated were: services per conception (x 2 ), days from parturition to first estrus, and days open (Tuckey’s test).
There was no difference in body condition score among groups at parturition or during the postpartum period (P>0.05). There was also no difference in the number of services per conception. The average number of days from parturition to first estrus were 73.38±15.56 a ; 64.42±17.05 b and 65.34±18.31 b , and the average number of days open were 97.34±26.54 a , 86.38±28.81 b and 85.23±30.12 b for groups 1, 2 and 3, respectively (P
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130. évfolyam Különszám II. Vol
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The World Buiatrics Congress (WBC)
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lood and liver tissue. Absolute vit
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RNS causes increased lipid peroxida
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An experiment was conducted to stud
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2 Hungarian Institute of Agricultur
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and plasma HCO 3 - increased from H
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Materials and Methods: Serum sample
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vitamin A stores in liver, the hypo
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andomly assigned to one of five tre
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observed in group II of the animals
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The aim of the monitoring was to co
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health group (HG) categories for an
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lactating cows is sparse. Lotan and
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491 Effect of Acarbose on Milk Yiel
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499 Secondary Hepatogenous Photosen
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they all died in a two to three day
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and HCO 3 level. The concentration
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amount of saponins was observed in
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Water was offered ad-libitum. Feed
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Se, I and Co) more than 500 blood s
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541 Variability of Blood Profile an
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evaluated, highly significantly gre
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we measured sensitivity of the enti
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were 0.17; 0.13 °C (Max.: 0.73). D
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calving pregnancy period were the b
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2 University of Nottingham, Departm
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animals) was treated with 5 mg enro
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In dairying, good management practi
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Somatic cell count (SCC) is one of
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This investigation was run in some
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eproductive data showed that cows f
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(3.6%) and others (Bacillus spp., N
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602 Coagulase Gene Polymorphism of
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2 Institute of Veterinary Bacteriol
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an important part of the pathogens
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4 Universidade de Sao Paulo, Clinic
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incubated for one hour at 37 oC. Af
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In Belgium, an increasing amount of
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Conclusion: It can be concluded tha
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calves recovered after 4 to 10 days
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disease. Thus losses were estimated
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635 Prevalence of the Pulmonary Les
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Conclusions The sanitation program
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Italy. After the sample examination
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Results: The cows sera were collect
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(Mb) as co-infecting agent is becom
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distributed over 13 different fatte
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main infection sources to the urban
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686 Characterisation of Cattle Orig
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using EPFC. The intradermal tubercu
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three cattle farms each. Leptospiro
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(cELISA) only to definitively confi
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1 Islamic Azad University of Shahre
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Financial support: CNPq, CAPES, FIN
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731 Detection and Investigation on
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necropsied, tissue samples were tak
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aseline values. The mean HCO 3, PCO
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immunization with either the curren
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decrease in animal aggregation in p
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747 Effect of Dietary Supplementati
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755 Study of Pulmonary Adenomatosis
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1 University of Erciyes, Faculty of
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2006 was observed a small decreasin
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for O69 strain), and clinical signs
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2 University of Veterinary Medicine
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794 Serum Haptoglobin and Fibrinoge
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complete random design (CRD). The r
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- Ca, Mg, K, and Ca:P ratio were lo
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818 Cerebral Coenurosis in a Goat:
Page 142 and 143: concentrations of the goat milk, in
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Page 150 and 151: 847 Llama Anesthesia for Orthopedic
Page 152 and 153: 28 Preliminary Results of a General
Page 154 and 155: Israeli commercial dairy herds cons
Page 156 and 157: 109 Association between Johne’s D
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Page 160 and 161: In the last year 2006, in the perio
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Page 166 and 167: The level of antibodies produced in
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Page 174 and 175: Objectives: To evaluate two differe
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Page 178 and 179: 1134 A Comparison between Two Calf
Page 180 and 181: The present work aimed to investiga
Page 182 and 183: University College Dublin, School o
Page 184 and 185: 214 Cystic Ovarian Diseases in Dair
Page 186 and 187: inseminator were tested in regressi
Page 188 and 189: University of Nottingham, Animal He
Page 190 and 191: tissue (8,6 cm 2 ) was twice as lar
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Page 196 and 197: improve embryonic development are m
Page 198 and 199: in each sample). On day 0, 104 cows
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Page 202 and 203: and durable spermatozoa. Within the
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Page 208 and 209: 909 The effect of “GnRH” and
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Page 218 and 219: (I.M.) 1 mg of estradiol cipionate
Page 220 and 221: Lameness results in major economic
Page 222 and 223: methods; ANOVA (with binary classif
Page 224 and 225: Initially two radiographs were obta
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Page 230 and 231: Key words: calf, active immunity, p
Page 232 and 233: 1 University of Camerino, Veterinar
Page 234 and 235: vaccinated (Group II) with Pastobov
Page 236 and 237: # Antigens Association in diarrhea
Page 238 and 239: 1015 Prevalence of Eimeria Infectio
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thymus in calves and heifers with c
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concentrations of calves with umbil
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The goal of this paper is to expose
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187 Cooling Strategies to Improve M
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inspections, carried out by specifi
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The origin of Hungarian Grey cattle
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University of Warmia and Mazury in
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POSTER ABSTRACTS 1225 The Effect of
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multiple fracture (n=1, 1.3%). The
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position. A fusiform skin incision
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Conclusions: It is the first presen
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1 Faculty of Veterinary Medicine, A
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of 101.55±4.94 mmHg. Afterwards th
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In Mexico, there is a wide variety
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Cardiovascular disorders are common
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Conclusion: The total contamination
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Material and methods: The trial inc
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detection of M. wenyonii infection.
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390 Study on Attenuation Mechanisms
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POSTER ABSTRACTS 1152 Detection of
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digestion. It works only on Apicomp
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determined. In addition the surface
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Africa and Europe, which causes mor
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Results: On D0, the animals include
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addition of mineral salt, during 12
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digestion could be improved with im
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Ltd., Blandford Forum, UK). The air
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cattle ocular squamous cell carcino
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pharmacokinetic/pharmacodynamic (PK
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Bovine Immunodeficiency Virus (BIV)
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significantly lower in groups vacci
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5.00+0.67 and 4.94+0.15 mEq/l in th
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potable water and food, avoiding st
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equal distributions at 08:00 and 16
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electrochemical properties of the a
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Selenium was discovered as an essen
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growth by measuring BW at 15-day in
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influence of different risk factors
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performances of primiparous cows ar
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anaerobes. Aerobic bacteria isolate
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P4 12 to 24 hours before calving is
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exists about Mb prevalence in dairy
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frequency of animals with BoHV anti
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esults, supported by a decrease in
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international drivers of change. Th
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The performance of two groups of no
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spasm is accompanied by kyphosis, w
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occurrence of a crisis of stiffness
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Notices 336 XXV. Jubilee World Buia
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