Oral and Poster Abstracts

lipases (porcine pancreatic lipase type II and type VI-S, microbial lipase),
detergents (SDS 10%; Lubrol), and incubation periods (0.5-16 h) were
tested. Triolein (Sigma, Steinheim, Germany) was used as internal
standard. The coefficient of variance (CV) of TL analysis in the ten liver
bioptates (range: 40-314mg/g FW) was in average 2.2% (max. 4.5%;
three repetitions). For the extra incubation step prior to the final enzymatic
TAG analysis (MTI diagnostics, Idstein, Germany) microbial lipase,
Lubrol, and 16 hours of incubation provided best results. Mean recovery
of Triolein was 101% (97%-107%) with mean intra and inter day (10
samples, 5 repetitions) CV% of 0.75% and 2.7%, resp. The liver TAG
(range: 4-260 mg/g FW) analysis showed mean intra and inter day CV%
of 2.5% (0.35-5.6%) and 3.4% (2.3-4.9%), resp. The presented combined
method for TL and TAG determination in small amounts of bovine liver
tissue was simple, accurate and reproducible.
This work was supported by WILHELM SCHAUMANN STIFTUNG,
Hamburg, Germany.
288 Study on the Mechanism in the Damage of Erythrocyte
Membrane in Low-phosphorus Cows
SW. Xu, FQ. Shi, DW. Xuan
Northeast Agricultural University, College of Veterinary Medicine,
Harbin, China
Objectives: Investigate the mechanism in the damage of erythrocyte
membrane (EM) in low-phosphorus cows.
Materials and Methods: These cows with field cases were divided into
three groups, including hemoglobinuria group (HG), low-phosphorus
group (LPG) and control group (CG). The phospholipid composition,
skeletin, antioxidant function and shape of EM were determined.
Results: The phospholipid composition, skeletin, antioxidative function
and shape of EM obviously changed in HG and LPG. (1) Phosphatidylethanolamine
(PE) content in HG was significantly lower than
that in LPG and CG, but sphingomyeline (SM) and phosphatidycholine
(PC) + phosphatidylserine (PS) content in HG was significantly higher
than that in LPG and CG. In comparison between LPG and CG, PC + PS
content were lower and SM content was higher in LPG. Significant
positive correlation and negative correlation were observed between
serum phosphorus and PE content, serum phosphorus and SM content,
respectively. (2) There were no difference was found in EM skeletin
between LPG and CG. Spectrin I, spectrin II, and band IV-2 content was
lower in HG than that in LPG and CG, but band III was higher in HG than
that in LPG and CG. (3) SOD activity and GSH-Px activity in HG and
LPG was significantly lower than that in CG. MDA content in HG and
LPG was significantly higher than that in CG. There were a significant
positive correlation between serum phosphorus and erythrocytic SOD
activity and GSH-Px activity, and a negaitive correlation between serum
phosphorus and erythrocytic MDA content. (4) Through the observation
of scanning electron microscope, the course of erthrocytolysis attributable
to low phosphorus intake was proved: the erythrocytes changed from
biconcaval shape to acanthocytes, spherocytes and to be destroyed
eventually with the decresed serum phosphorus content. Treatment with
phosphorus preparation could signficantly alleviate the change in
erythrocyte shape and make it to return to normal shape.
Conclusions: The phospholipid composition, skeletin, and shape of EM
changed, and the antioxidant function of EM decreased in lowphosphorus
cows. These further caused the occurrence of the haemolysis.
Key words: cow; low-phosphorus; erythrocyte membrane
289 Prevalence of Digital Dermatitis in First Lactation Cows
Presented at Auction
M. Hulek 1,2 , I. Sommerfeld-Stur 3 , J. Kofler 1
1 University of Veterinary Medicine Vienna, Department of Horses
and Small Animals, Clinic of Orthopaedics in Large Animals,
Vienna, Austria
2 Veterinary Practice Michael Hulek,Oberneukirchen, Austria
3 University of Veterinary Medicine Vienna, Department of Animal
Breeding and Reproduction, Institute of Animal Breeding and
Genetics, Vienna, Austria
Objective: The risk of introducing digital dermatitis (= DD) to dairy
herds by the introduction of infected, newly purchased cattle is
assumed to be considerable. The aim of the study was to assess the
prevalence of DD in first lactation cows (FLCs) presented as breeding
cattle at the monthly auction in one auction centre in Austria over a
period of 10 months.
Material and Methods: The FLCs to be examined were selected
randomly for claw examination for each auction from the monthly auction
catalogue. At each auction a minimum of 25% of FLCs were selected,
with an average of 36% of all FLCs of all 10 auctions. After obtaining
owner’s consent, the hindclaws were examined in a walk-in crush for DD.
DD lesions were evaluated by the parameters localisation (plantar,
interdigital, dorsal aspect), diameter in cm and lesion type (M1-M4).
Other claw lesions and trimming status of the claws were also noted.
Results: From a total of 1110 FLCs registered for the ten auctions on
the catalogues, 399 were chosen for examination, and of these 199
FLCs could be examined. In 63 cows from the random sample the
owners did not consent to the examination. A total of 24 FLCs were
found to have DD lesions on one or both hindlimbs, with at least one
cow detected at nine of the ten auctions. The size of lesions ranged
from 0.5 to 3 cm in diameter. The prevalence of DD determined at 10
auction dates within 10 months was 12.06%.
Conclusions: The results of this study show that about 12% of FLCs
presented as breeding cattle at auction were affected with DD. This
result suggests that in the worst case, if each of these FLCs affected
with DD is introduced into a DD free herd, 24 new herds could be
infected by DD during a 10 months period from one single auction
centre in Austria. An additional risk is the infection of other animals at
the auction from the use of shared walkways and pens, with a
potentially much larger number of herds affected at a later stage. In
order to reduce both these risks, we recommend that dairy farmers
purchase only cows free from digital dermatitis. An examination of the
claws for the presence of DD lesions should be carried out in all cows
one or two weeks before they are presented at auction. This
examination should be performed by trained experts and the findings
should be documented in a special protocol, which should be presented
with the cow at the auction, and be available for inspection by all
potential buyers.
POSTER ABSTRACTS
443 Influence of Age, Season and Physiological State on some
Biochemical Parameters in South Eastren Algerian Desert
Goats
H. Nadia 1 , M. Toufik 1 , M. Bakir 2 , B. Mabrouk 1
1 Batna University, Veterinary, Batna, Algeria
2 Batna University, Virology, Batna, Algeria
Blood plasma Ca, P, Mg, Na, K, and Fe were analysed in order to
establish biochemical references, and to study the influence of age,
season and physiological state on the variation of these parameters.
The results demonstrate:
– Ca, Mg, and Na levels were high at birth and decrease with age.
– The season had a signficant effect on the levels of these ions, for
example Ca, Mg, K decreased and inversely Na increased during dry
season.
– The values obtained for the plasma Ca, Mg, Na, K, Ca:P and Fe levels
in pregnant goats were 80.02±4.84 mg/l, 22.14±1.61 mg/l, 142±1.73
mEq/l, 6.43±0.40 mEq/l, 1.29±0.37 and 91.11±18.84 µg/100ml
respectively. They were significantly higher than in non pregnant and
lactating females.
– Ca (77.67±3.20 mg/l), Mg (20.44±1.66 mg/l), K (5.65±0.57 mEq/l)
and Ca:P (1.35±0.35) were lower in lactating goat compared to
pregnant and non pregnant goats.
These values of plasma minerals can be used as reference to detect
metabolic and/or nutritional disorders in goat.
Key words: mineral metabolism, goat, lactating, desert
44 The Evaluation of Vitamin A and ß-carotene Levels during
Postpartum Period in Semi Industrial Dairy Farms in Iran
M. Tehrani Sharif 1 , R. Mozaffary 2 , J. Gholami Seyed kolaee 3 ,
M. Rezaee ghale 2 , A. Cheraghzadeh 2
1 Islamic Azad University , Garmsar branch, Department of
Pathobiology , Faculty of Veterinary Medicine, Garmsar, Iran
2 Islamic Azad University , Garmsar branch, Student, Garmsar, Iran
3 Shahid Chamran University, Student, Ahwaz, Iran
Objective of study: Vitamin A, retinol, plays a vital role in vision sense.
Due to the presence of large amounts of beta-carotene in cattle’s foods &
Nutrition and Metabolic Disorders 15