Oral and Poster Abstracts

Results: The cows sera were collected from Kurdistan province of
Iran. By SN test the rate of infection in all samples was 27.7%. This
result done with similar study was approximately like that one carried
out on 1357 serum samples from other provinces in Iran.
Conclusions: Being on the border and consequently animals entering
the country explains the high rate infection in Kurdistan province.
Key words: viral diarrhea, serum neutralization, Sanandaj
658 Treatment of Infectious Diseases in Sheep with Enrofloxacin
10% (Kinetomax) - Case Reports
MI. Ferreira 1 , EJ. Facury Filho 2 , AU. Carvalho 2 , I. Borges 1 ,
G. Macedo Junior 1 , N. Martins 3
1
Escola de Veterinária da UFMG, Departamento de Zootecnia, Belo
Horizonte, Brazil
2
Escola de Veterinária da UFMG, Departamento de Clinica e
Cirurgia Veterinaria, Belo Horizonte, Brazil
3
Escola de Veterinária da UFMG, Departamento de Medicina
Veterinária Preventiva, Belo Horizonte, Brazil
Enrofloxacin 10% (Kinetomax) is a potent chemotherapist agent
showing bactericidal action. It has a broad spectrum of antimicrobial
action presenting rapid absorption, distribution and metabolism in the
body. Thirty-two crossbred LacaunexSanta Ines sheep were allocated
to individual stalls and kept in confinement in the Laboratory of
Animal Calorimetry and Metabolism, School of Veterinary Medicine
at UFMG, for 120 days in lactation for researching on production and
composition of milk. Case 1: seven animals - five sheep and two lambs
- had acute inflammatory reaction of the conjunctiva; sclera hyperemia;
photophobia; blepharospasm; lacrimation; mucopurulent ocular
discharges; and corneal opacity, without ulceration, resembling
keratoconjunctivitis. Enrofloxacin 10% (Kinetomax) was used for
treatment in accordance with the recommendation of 7,5 mg/Kg /b.w.,
in all affected animals. On the fourth day after application of the
product, the reduction of clinical symptoms was observed, with
complete recovery of the animals. Prior to the treatment onset, samples
of ocular secretion were collected, with the help of sterile swabs, and
sent to the Laboratory of Microbiology at the Department of Preventive
Veterinary Medicine, where Staphylococcus caprae was isolated. Ten
days after treatment, two animals showed clinical relapse and the
occurrence of two new cases. The animals were subjected to treatment,
following the fore-cited protocol. On the fourth day after the second
treatment, there was complete reduction of clinical symptoms. Case 2:
monitoring the daily consumption, a gradual decrease of food intake
and reduced milk production of one animal was observed for five
consecutive days. In the clinical examination, there was an increasing
in volume, redness, and heat on the crown of the left forelimb hoof
(coronary band), so that the animal could not stand, featuring an
interdigital phlegmon (footrot), in initial state of development. For
treatment, enrofloxacin 10% (Kinetomax) was used at a dose of 7,5
mg/Kg /b.w., along with flumexine meglumine at the dose of 2,2
mg/Kg. Hoof trimming was made in the hoofs where no deeper injuries
were found. On the following days, there was an increase of food intake
and the recovery of the clinical signs was complete on the third day
after treatment.
Key words: footrot, keratoconjunctivitis, Kinetomax, sheep
659 Efficacy Following a Flexible Dose Regimen of Spirovac R , a
Leptospira Borgpetersenii Serovar Hardjo (Type Hardjobovis)
Bacterin, in a Challenge Study in Cattle
D. Cleary 1 , S. Behan 1 , J. Wong 1 , M. Fisher 1 , S. Saginala 1 , L. Kopta 1 ,
M. Tory 1 , A. Weber 1 , G. Gallo 1 , R. Leyh 1 , J. Galvin 1 , J. Salt 2
1
Pfizer Animal Health, Veterinary Medicine Research and
Development, Kalamazoo, Michigan, United States
2
Pfizer Animal Health, Animal Health Research Division, Sandwich,
United Kingdom
A research study was conducted to demonstrate the protection in cattle
afforded by a flexible dosing regimen using a Leptospira
borgpetersenii serovar Hardjo (type hardjo-bovis) bacterin (Spirovac,
Pfizer Animal Health). All animal care and procedures were approved
by an ethical review board for compliance with animal welfare
guidelines. Spirovac (n=20) or placebo (n=11) treatments were
randomly assigned to 4-week-old L. borgpetersenii seronegative
[microscopic agglutination test (MAT) antibody titers of [1:12.5] heifer
calves. The Spirovac’ group of calves received a second bacterin dose
90 XXV. Jubilee World Buiatrics Congress 2008
a minimum of four months later. Three weeks following administration
of the booster dose, all animals, including the placebo-vaccinated
control group, were challenged for three consecutive days via
conjunctival instillation of a heterologous strain of L. borgpetersenii.
Urine samples were collected for bacterial culture up to five weeks
following challenge when the animals were humanely euthanized and
kidney samples were collected. All animals were seronegative to L.
borgpetersenii at the time of first vaccination and all placebo control
animals were seronegative at the time of the challenge. The primary
variable utilized for the evaluation of protection was bacterial isolation
(presence or absence) of L. borgpetersenii in the urine following
challenge and in the kidneys at necropsy. Ninety-one percent (10/11) of
the placebo control animals were positive for L. borgpetersenii
isolation in both urine and kidney samples, confirming a valid
challenge. No L. borgpetersenii was isolated from the urine or kidneys
in the group receiving two doses of bacterin (0/20). These results
confirm that two doses of Spirovac administered approximately four
months apart can protect calves against virulent challenge.
Key words: Leptospira hardjo, Spirovac, vaccines, immunology, cattle
660 Survey on Dairy Cows Infective Abortions in the North-East
of Italy
A. Barberio 1 , N. Pozzato 1 , L. Ceglie 2 , S. Nardelli 2 , M. Badan 1 ,
G. Vicenzoni 1
1
Istituto Zoop. Sper. Venezie, Sezione Verona e Vicenza, Vicenza,
Italy
2
Istituto Zoop. Sper. Venezie, Dipartimento Sanita’ Animale, Legnaro
(PD), Italy
Objectives of study: The purpose of this study is to describe the results
of the abortion surveillance program in Veneto region (North-East of
Italy) over a period of two years.
Materials and Methods: Aborted fetuses accompanied by the dam’s
blood sample were delivered to the Regional Veterinary Laboratory
(IZS), and submitted to a panel of laboratory tests. The cows sera were
tested for antibodies against Brucella, Neospora, IBR, Leptospira, and
BVD non structural protein NS2-3. ELISA tests were used for
Neospora (VMRD), BVD (Pourquier) and IBR (Bommeli) antibodies
detection, while microagglutination with the method of Martin and
Petit was used for Leptospira. Brucella antibodies were detected
following the EU regulation mandatory method. Necroscopy and
routine bacteriology were performed on all fetuses, while Brucella spp
isolation was carried out only if the abortion occurred after the 5th
pregnancy month; Campylobacter fetus isolation was performed on
abortions occurred before the 5th month. In case of positive results for
BVD or N. caninum antibodies in mother serum, identification of these
microorganisms in fetus was performed with RT-PCR for BVDV and
PCR for Neospora.
Results: During year 2005 and 2006, 577 abortions were delivered to
IZS laboratories: 30% of abortions (170) were due to infective
microorganisms and among these N. caninum was the most frequent
(63%). Among the other microorganisms found, BVDV was isolated in
14% of positive fetuses, Arcanobacterium pyogenes in 9%, and
Listeria monocytogenes in 4%. Considering that only fetuses aborted
by serological positive cows were tested with PCR for N. caninum, the
percentage of fetuses found infected by this parasite was very high
(47%), and is in agreement with the reports indicating that cows with N.
caninum antibodies are more likely to abort than seronegative cows.
Serological tests showed a high prevalence of antibodies against BVD
virus (54%), Neospora caninum, (39%), and IBR virus (32%). On the
contrary there was a low frequency (3%) of positive sera for
Leptospira, and among the 16 positive samples, only two cows had a
serological titer high enough to be considered the consequence of a
recent infection.
Conclusions: In our opinion the abortion surveillance program
provided many useful and interesting information about the health
status of dairy farms and the activities to be performed to prevent
abortion diseases in the future, besides guaranteeing a mandatory
requirement for the declaration of officially brucellosis-free region.
661 Severe Outbreak of Malignant Catarrhal Fever in a Dairy
Herd
T. Autio 1 , P. Syrjälä 2 , M. Hautaniemi 3 , U. Rikula 3 , S. Pelkonen 2
1
Finnish Food Safety Authority Evira, Production Animal Health,
Kuopio, Finland