Oral and Poster Abstracts

motility and CM integrity (p=0.869 and p=0.892 respectively,
Friedman Test), in the three concentrations studied. Amicacine
sulphate was confirmed as a good option for the control of semen
microbiota mainly in IVF embryo production.
This paper was supported by FAPESP: Fundaçio de Amparo a Pesquisa
do Estado de Sao Paulo, Brazil
Key words: amicacine sulphate, spermatozoa viability, membrane
integrity
895 Efficacy of a Treatment with hCG 4 Days after AI to Reduce
Pregnancy Losses in Lactating Dairy Cows after
Synchronized Ovulation
C. Fischer-Tenhagen 1 , G. Thiele 1 , W. Heuwieser 2 , B. Tenhagen 3
1
Tierarztpraxis Thiele, Baruth, Germany
2
Freie Universität Berlin, Tierklinik für Fortpflanzung, Berlin,
Germany
3
Bundesinstitut für Risikobewertung, Infection epidemiology and
zoonoses, Berlin, Germany
The objective of the study was to investigate, whether a treatment
with hCG 4 d after AI could reduce pregnancy losses in high yielding
dairy cows. Pregnancy rates (PR) and pregnancy losses were
compared in four groups of cows. Cows of one large dairy herd
presented to the veterinarian in a fixed reproductive management
protocol were treated with an Ovsynch protocol if no corpus luteum
(CL) could be palpated per rectum (Group OV). Cows with a CL
received cloprostenol (0.15 mg) and buserelin (0.01mg) 2 d later and
timed AI 16 to 20 hrs later (Group PG). Cows in both treatment
protocols were then assigned to two groups by the final digit of their
identification number (even vs. odd numbers) to receive 2,500 IU of
hCG i.v. four days after AI (Groups OV-hCG, OV-Control, PG-hCG
and PG-Control). Pregnancy diagnosis was carried out 27 d after AI
via ultrasound and 39 d after AI by rectal palpation. Pregnancy
losses were defined as cows being pregnant on d 27 but not pregnant
on d 39 after AI. Pregnancy rate by d 27 did not differ between the
four treatment groups (35.4, 35.0, 37.0 and 38.0 % for Groups OVhCG,
OV-Control, PG-hCG and PG-Control, respectively).
Pregnancy losses between d 27 and d 39 after AI were smaller in
hCG treated animals in the summer months, but did not differ in
autumn and spring. Pregnancy rate by d 39 after AI were higher in
PG than in OV groups, but independent of hCG-treatment. In
conclusion, treatment with hCG 4 d after AI did not significantly
increase PR 39 d after AI. A positive effect of hCG on pregnancy
losses during the summer months warrants further investigation.
896 The Influence of Inmodulen on PMN and L activity and
Antioxidative Potential in Blood of Cows
H. Markiewicz, M. Gehrke, E. Malinowski, M. Kaczmarowski
National Veterinary Research Institute, Department of Pathophysiology
of Reproduction and Mammary Gland, Bydgoszcz, Poland
The aim of the study was to establish of the best term of the second
injection of Inmodulen and evaluate the influence of its on oxygenic
metabolism of neutrophils and antioxidative potential in blood of
cows in the puerperal period.Preliminary examinations were
conducted on 20 healthy, non pregnant cows between 80 and 100 day
of lactation. Ten cows received a single i.m. injection of 10 ml of the
Inmodulen and the remaining 10 cows constituted the control group.
The blood samples from experimental and control cows were taken
from the tail vessels on day 0, 7, 14, 21. The level of both
spontaneous and induced luminodependent chemiluminescence (CL)
was determined. It was stated that single injection caused a
significant increase of spontaneous and induced CL on the 7 th and
14 th day (vs. the “0” day), which decreased on 21 st day after
injection. The highest CL was detected on day 14, with exception of
OZ (similar level was on day 7 and 14). On the basis of these results
it has been suggested that the second application of the preparation
should be made 14 days after the first application. The basic
investigations were carried out on 35 cows: 18 experimental
receiving Inmodulen and 17 controls. Inmodulen was injected on the
day of parturition and on the 14th day thereafter. The blood was
sampled once a week during the first 5 weeks of lactation. The level
of CL, phenotype of subpopulation of T lymphocytes (CD2, CD4,
CD8) with flow cytometry, the TAS of plasma as well as SOD, GPx,
and CAT were examined. A significant influence was demonstrated
202 XXV. Jubilee World Buiatrics Congress 2008
of both the preparation and the time after parturition on the increase
of the value of spontaneous and induced CL. The time of
examination was important for CD2 lymphocytes, whereas the
preparation and the time after parturition influenced CD4
subpopulation. CD8 subpopulation did not change. The time of
samples collecting had a significant influence only on the activity of
TAS and SOD unlike GPX whose levels depended on the
administration of Inmodulen.The level of TAS and SOD
systematically increased from 1 to 5 week, unlike CAT whose level
was the same all time. It was stated that Inmodulen i.m. injected on
the day of parturition and 14 days thereafter, resulted in the
significant increase of CL, which directly points on an increase of
bactericidal activity of PMNs. The activation of these cells did not
influence on the pro-antioxidative equilibrium of plasma and the
activity of SOD and CAT, however, it increased of GPx activity.
Key words: cows, PMN, chemiluminescence, antioxidative potential,
Inmodulen
897 Endometrial Changes in Postpartum Period in Cows
I. Sematovica 1 , M. Pilmane 2 , A. Jemeljanovs 1 , D. Ikauniece 1
1
Research Institute of Biotechnology and Veterinary Medicine Sigra
of the Latvia University of Agriculture, Sigulda, Latvia
2
Riga Stradins University, Institute of Anatomy and Antropology,
Riga, Latvia
The aim of the research was to reveal inflammatory factors,
neuropeptide-containing innervation, distribution of growth
stimulating, degradation factors, and apoptosis in the cows’
endometrium in postpartum period. Nine multiparious cows after
normal calving were biopsied twice in the first and the fifth week
after parturition. Matrix metalloproteinases (MMP2 un MMP9,
1:100, R&D, England), tumor necrosis factor (TNF, 1:100, Abcam,
England), interleukin 10 (IL10, 1:400, Abcam, England), vascular
endothelial growth factor (VEGF, 1:50, DakoCytomation,
Denmark), nerve growth factor receptors (NGFR p75, 1:150,
DakoCytomation, Denmark), protein gene product (PGP 9.5, 1:1600,
DakoCytomation, Denmark) were immunohistochemically detected,
and the TUNEL method was used for detection of apoptosis (PCD).
Haematoxylin and eosin staining was performed for each sample.
Hyperemia, different level of infiltration of tissue with inflammatory
cells, oedema, detachment of the lining epithelium, and erosive
surface of mucous membrane in separate samples were observed in
the first week after parturition. Four weeks postpartum, hyperplasia
of basal cells, hyperemia, proliferation of endometrial glands,
perivascular and periglandular infiltration with inflammatory cells,
as well as lymphatic follicules were found. There were detected a
significant increase of the number of inflammatory cells, TNF
amount, and expression of VEGF, NGFR p75. A mild positive
correlation was observed between the amount of inflammatory cells
and TNF, PCD and TNF, TNF with NGFR p75, NGFR p75 and PGP
9.5, VEGF and PCD, VEGF and NGFR p75. A strong positive
correlation was seen between TNF and VEGF, as well as VEGF with
NGFR p75. The increased expression of NGFR p75, VEGF and TNF
seems to correlate with the ischaemia of tissue increased by
inflammatory action in the cows endometrium from the first up to
the fifth week postpartum. The latter also seems to stimulate the
apoptosis and proliferation of nerve fibres in the cows endometrium.
Key words: uterus, after parturition period, cows
898 Efficacy of Three Antibiotic Protocols Added to Bull Semen
Extender for the Inactivation of Leptospira Spp. Serovars
Hardjo, Wolffi and Grippothyphosa
M. Genovez 1 , T. Gotti 1 , V. Castro 1 , C. Horbach 2 , F. Viana 3 ,
S. Azevedo 4
1 Instituto Biológico, Animal Health Center, Sâo Paulo, Brazil
2 Universidade Estadual Paulista, Campus Botucatu, Animal
Reproduction and Veterinary Radiology, Botucatu, Brazil
3 Universidade Federal De Campina Grande, Department for
Infectious diseases- Epidemiology, Patos, Brazil
4 sersia Brasil, Animal Reproduction &Ai, Bragança Paulista, Brazil
Two approaches can be applied to assure the safety of semen in
AICenter: continuous surveillance of bulls before and after semen
production and end product checking. The World Organization for
Animal Health recommends adding at least one antibiotic treatment