Oral and Poster Abstracts

3 Clínica Privada, México DF, Mexico
4 UAEM, Toluca Edo, MEXICO, Mexico
The study aims to find out the situation of leptospirosis through a
bibliographic national analysis considering the ecological regions in
Mexico. The articles were chosen according to the following criteria: a)
diagnosis technique: microscopic agglutination, b) positive criterion
1:100 or more, c) time period: 1991-2003, d) type of publication (such
as thesis, conference reports, well-known and certified magazines) e)
location according to the state. Duplicated information was excluded.
The results of frequency and serovars of leptospirosis were reported in
each state , according to ecological regions: dry and semi-dry, warm, dry
and humid tropic. The results include 17 of 31 states. Antibodies against
19 different serovars and seropositive range from 49.7% to 42,779
serum were analysed. The dry and semi-dry region had a 37.8%
frequency (range from 31% to 59%), the most prevalent serovars were
Hardjo genotype hardjoprajitno strain H-89, Hardjo, Wolffi y
Tarassovi. Warm weather: the average frequency of leptospirosis was
39.4% (from 22% to 66%). The most prevalent serovars were
Icterohaemorrhagiae strain Palo Alto, Canicola serovar Portland-vere,
Bratislava, Pyrogenes; Pomona; strain H-89; Hardjo; Wolffi y
Tarassovi. Dry tropic: 45.9% (27% to 72%), the most prevalent serovars
were Wolffi, Hardjo y Tarassovi. Humid tropic: the average frequency
was 63.8% (from 31.7% to 84.6%), the most prevalent serovars were
Hardjo genotype hardjoprajitno strain H-89, Hardjo, Wolffi y
Tarassovi. We conclude that leptospirosis in cattle is endemic in
Mexico. This suggests a high serological frequency, and that ecological
factors can determine the prevalence of the disease. The most frequent
serovars in bovines are Hardjo hardjoprajitno strain H-89, Hardjo
hardjoprajitno international reference strain, Wolffi and Tarassovi.
Key words: leptospira, leptospirosis, Mexico, Hardjo, cattle.
1116 Sanitary Management Related to the Presence of Antibodies
against Leptospira, Brucella and Neospora in Cows from a
Dairy Farm
M. Botello 1 , A. Yaez 1 , J. Torres 1 , L. Moles 1 , D. Gavaldon 2
1
Universidad Autónoma Metroopolitana Unidad Xochimilco,
Producción Agrícola y Animal, México DF, Mexico
2
Universidad Autónoma Metroopolitana Unidad Xochimilco, México
DF, Mexico
This study aims to determine serologically the leptospirosis, brucellosis
and neosporosis sanitary status in a dairy farm of 300 animals, located
in the central high plateau of Mexico. The sanitary management
comprises colostrums intake after birth and maternal milk feed during
the 6 first days, intensive medical care and the appropriate treatment
when a disease is diagnosed, as well as diagnostic tests when ever are
required. It is important to point out that most of the calves are retained
in the dairy farm to replace the remained cows for milk production
porpoises so there is no animal introduction. There are different zones
in the farm as the calf breeding, gestation and parturition and the milk
production areas, so there is no animal contact between one each other,
the semen used for artificial insemination has a sanitary certification.
The 300 bovines were sampled for leptospiral diagnosis with 13
serovars using the microscopic agglutination test considering 1:100
dilution or higher as a positive titer, brucellosis with bengal rose test
and ELISA for neosporosis. All the animals tested were serologically
negative to the 3 diseases studied and it is probably due to the adequate
sanitary management and the proper installations in the farm that avoid
the presence and dissemination of the infectious agents of these 3
diseases at least. It is convenient to mention that an adequate sanitary
management is necessary to keep a dairy farm free of some infectious
agents that are evolved in reproductive disorders.
Key words: leptospira, brucella, neospora, cattle, Mexico
1117 Lymphocyte Proliferation in Bovine Leukemia Virus-
Intected Dairy Cows
F. Souza 1 , M. Azedo 1 , M. Sakai 2 , K. Kieling 2 , R. Weigel 1 ,
B. Sanches 1 , C. Strincagnolo 1 , M. Blagitz 1 , C. Massoco 3 ,
AM. Della Libera 1
1 Faculty of Veterinary Medicine and Animal Husbandry FMVZ-USP,
Clinica Medica, Sao Paulo, Brazil
2 Faculty of Veterinary Medicine and Animal Husbandry FMVZ-USP,
Department of Pathology, Sao Paulo, Brazil
3 Cornell University, Clinical Sciences, Ithaca, United States
The purpose of the present trail was to determine the lymphocyte
proliferation in dairy cows infected with bovine leukemia virus (BLV)
with distinct lymphocyte profiles in infected animals known as
alymphocytotic (AL) and persistent lymphocytosis (PL). Thus, 202
Holstein cows were sera tested by agar gel immunodiffusion (AGID)
and enzyme-linked immunosorbent-assay (ELISA), 47 days before and
at the day of the lymphocyte proliferate assay. Besides this, the
leukocyte profile was evaluated to discriminate these distinct groups
(AL and PL). The lymphocyte proliferation was done with no
stimulation (basal), concavalin-A (Con-A) (100 mg/ml) and
lipopolysaccharide from Escherichia coli serotype O127:B8 (LPS) (250
mg/ml) when 2x10 6 /ml lymphocytes were plated per well and analyzed
by flow cytometry. The results on serologic test showed 51% positive in
both AGID test and ELISA, but in ELISA test about 38% were classified
as suspect. From these positive animals in both tests 15.60% presented
LP. From these animals, 15 cows were selected and divided uniformly
in 3 groups (negative, AL, LP). The mean (± standard deviation) total
leukocytes counts were 12.04 (±2.51), 11.78 (±0.62) and 35.30 (±11.14)
x10 9 /L (p