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Oral and Poster Abstracts

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1134 A Comparison between Two Calf Breeding Systems <strong>and</strong> its<br />

Influence in Weight Gaining at Weaning<br />

ML. Botello, JI. Torres, LP. Moles, D. Galvadon<br />

Universidad Autonoma Metropolitana-Xochimilco, Producción<br />

Agrícola y Animal, México D.F., Mexico<br />

The goal of the research is to contrast two breeding systems as a well as<br />

the nutritional value of two nutriments given to calves in order to<br />

determine the weight gaining at weaning. The study was carried out in<br />

two Holstein dairy farms with similar environments <strong>and</strong> managerial<br />

strategies. The colostrum given during first three days colostrum from<br />

one, two <strong>and</strong> three parturitions cows was given to the group (A) during<br />

the first three days after birth. The calves were provided with<br />

commercial food from this point until the 80 th day. Meanwhile group B<br />

was fed colostrum from three parturition cows for two days <strong>and</strong><br />

commercial food up to the 90 th days. A bromatological analysis was<br />

carried out to colostrum, whole milk, commercial food <strong>and</strong> dried<br />

alfalfa. In order to determine the average of body weight gained 15<br />

newborn <strong>and</strong> 15 weaned calves were measured. The colostrum’s<br />

infrared spectroscopical analysis gave the following results: group A<br />

had 12.03% proteins, 3.46% fat, 3.45% lactose <strong>and</strong> total solids 19.63%.<br />

Whereas group B displayed 19.86% proteins, 3% fat, 3.22% lactose<br />

<strong>and</strong> 27.48% total solids. The proximal analysis of the commercial food<br />

<strong>and</strong> dried alfalfa showed for the group A 18.35 % crude protein (CP),<br />

19.05% crude fat (CF), 0.13% crude fiber (CFi) <strong>and</strong> pellet milk’s<br />

substitutes 28% CP, 10% CF <strong>and</strong> 1% CF, commercial food 18.97% CP,<br />

0.10.86% CF, 3.64% CFi, 71.66% nitrogen free elements (NFE) <strong>and</strong><br />

5.69% ashes (A), dried alfalfa 24.5% CP, 0.0892% CF, 20.79% CFi,<br />

43.06% NFE, 11.54% A. The group B milk substitute 20% CP, 21%<br />

CF, 0.15% CFi, dried alfalfa 25.6% CP, 2.7% CF, 20.1% CFi, 36.1%<br />

NFE, 11.9% A. The commercial food reports 25% CP, 3% CF, 3% CFi.<br />

The weight gained in group A was 51.9 Kg <strong>and</strong> group B 62.250 Kg.<br />

Group B complies better with the minimal nutritional requirements<br />

than group A. The data suggest that a good quality milk substitute<br />

works better than whole milk because satisfies the minimal<br />

requirements at a lesser cost. The former secures quick ruminal<br />

development al weaning. Getting to the adequate body weight requires<br />

good management during lactation stage. It is always desirable to<br />

supply high quality colostrum during the calf’s growing stage.<br />

Key words: breeding systems, weight gaining, calves<br />

1135 Anti Leptospiral Antibodies in Equines <strong>and</strong> Canines in an<br />

Endemic Area of Bovine Leptospirosis<br />

C. Mendez 1 , JI. Torres 1 , P. Melendez 1 , L. Benavides 2<br />

1 Universidad Autonoma Metropolitana-Xochimilco, Producción<br />

Agrícola y Animal, México DF, Mexico<br />

2 Universidad Autonoma Metropolitana-Xochimilco, Sistemas<br />

Biologicos, México D.F., Mexico<br />

We pretended to identify specific antileptospiral antibodies in equine<br />

<strong>and</strong> canine sera from an area of endemic bovine leptospirosis caused<br />

mainly by serovars Hardjo <strong>and</strong> Tarassovi. We essayed 222 sera from<br />

bovines: 70 in gestation, 83 non gestating <strong>and</strong> 69 calves; 24 equine sera<br />

<strong>and</strong> 6 canine sera, all from Soto la Marina, Tamaulipas state in Mexico.<br />

The antileptospiral titration of the sera was performed by the micro<br />

agglutination (MAT) technique using thirteen serovars of Leptospira as<br />

antigens: Double dilutions of the sera starting in 1:50 were done, the<br />

titer was considered as the maximal dilution of sera in which 50% of<br />

agglutination of the antigens was observed. The results with the bovine<br />

sera were: 48 (68.57%) positives from the pregnant cows, to one or<br />

more serovars, the more frequent ones: Hardjo strain H89, 40<br />

(57.14%); Hardjo prajitno 36 (51.42%), Wolffi 34 (48.57%) <strong>and</strong><br />

Tarassovi 18 (25.71%). From the non gestating ones 52 (62.65%) were<br />

positives to one or more serovars, principally to Hardjo H89 38<br />

(45.78%) <strong>and</strong> Wolffi 18 (21.68%). The calves had 79.71% (55) of<br />

seropositivity, being Hardjo prajitno y Hardjo H89 the highest (51 or<br />

73.91%) <strong>and</strong> Wolffi 28 (40.58%). The horse sera reacted against one or<br />

more serovars, 22 positives (91.6%) mainly against Tarassovi<br />

11(54.83%), Hardjo prajitno 9 (37.5%), Bratislava 7 (29.16%) <strong>and</strong><br />

Canicola 7 (29.16%). The entire dog sera were positives to one or more<br />

serovars especially to Canicola 6 (100%), Portl<strong>and</strong>-Vere 6 (100%),<br />

Icterohaemorrhagiae Palo Alto 6 (100%), Icterohaemorrhagiae 4<br />

(66.6%) <strong>and</strong> Pyrogenes con 3 (50%). As in other parts of the Country,<br />

we confirmed the incidence of bovine leptospirosis in Soto la Marina<br />

specially the serovar Tarassovi <strong>and</strong> the related ones from the Serjöe<br />

176 XXV. Jubilee World Buiatrics Congress 2008<br />

Serogroup. Since the equine sera reacted mainly with serovar<br />

Tarassovi, we need to establish the importance of this species as a<br />

source of infection <strong>and</strong> transmission of this strain of Leptospira. This<br />

type of studies are relevant because of the close contact that horses<br />

have with bovines in this region <strong>and</strong> since it is not known the role that<br />

this species play in the transmission of serovar Hardjo to the bovines.<br />

Key words: antileptospiral, antibodies, equines, caninen <strong>and</strong> bovines<br />

1136 Antileptospiral Antibodies in Bovine <strong>and</strong> Human<br />

Populations Ina Dairy Farm in Tizayuca, Hidalgo Mexico<br />

JI. Torres 1 , L. Benavides 2 , P. Melendez 1 , D. Galvadon 1 , ML. Botello 1<br />

1 Universidad Autonoma Metropolitana-Xochimilco, Producción<br />

Agrícola y Animal, Mexico, D.F., Mexico<br />

2 Universidad Autonoma Metropolitana-Xochimilco, Sistemas<br />

Biologicos, México D.F., Mexico<br />

The objective of this work was to compare the serological profiles<br />

against leptospira in the human <strong>and</strong> bovine populations from a dairy<br />

farm near Mexico City. From an approximate population of 27,000<br />

milk producer bovines from the dairy farm of Tizayuca (TDF) in the<br />

Hidalgo state, Mexico; 240 bovine sera <strong>and</strong> 45 sera from farm workers<br />

<strong>and</strong> veterinarians, with one to 20 years of work in the farm, were<br />

sampled. The antileptospiral titration of the sera was performed by the<br />

microagglutination (MAT) technique using twelve serovars of<br />

Leptospira as antigens: Bratislava Jes-Bratislava, Canicola Hond<br />

Utrecht IV, Portl<strong>and</strong>-vere Sinaloa ACR, Grippotyphosa Moskva V,<br />

Icterohaemorrhagiae RGA, Icterohaemorrhagiae Palo Alto, Pomona<br />

Pomona, Pyrogenes Salinem, Hardjo Hardjobovis LT 1085, Hardjo<br />

Hardjoprajitno H-89, Wolffi 3707 <strong>and</strong> Tarassovi Perepelitsin. The titer<br />

was considered as the maximal dilution of sera in which 50% of<br />

agglutination of the antigens was observed. From 240 bovines sera,<br />

61%(147) reacted against on or more of the serovars used as antigens in<br />

the MAT, the most frequent was Hardjo Hardjoprajitno H-89 (isolated<br />

from TDF in 1989) with 42% (101); Hardjo with 27%(65); Wolffi con<br />

21%(51) <strong>and</strong> Tarassovi con 16%(39). In the 45 human sera analyzed<br />

we could not detect any reaction with the serovars used in the MAT.<br />

From the reactivity observed in the bovine sera against the serovars<br />

Hardjo <strong>and</strong> Wolffi from the serogroup Serjoe of Leptospira indicate<br />

that these serovars are in active circulation in the bovine population of<br />

the farm. The Hardjo serovar has been reported as the principal<br />

responsible of abortions <strong>and</strong> mortinates, weak calves at the birth,<br />

infertility <strong>and</strong> low milk production. In humans the main source of<br />

infections are the rodents <strong>and</strong> occasionally other mammals as may be<br />

the bovines. The fact that in this study the serological profiles of<br />

antileptospiral antibodies of bovines <strong>and</strong> men in the TDF did not<br />

coincide led us to think that L. Hardjo is well adapted to the bovine but<br />

not to man, even when he is working in close contact with active<br />

infected animals.<br />

Key words: antileptospiral antibodies, bovine, human, Mexico<br />

1137 Association of Bovine Viral Diarrhea Virus (BVDV) <strong>and</strong><br />

Bovine Herpesvirus-1 (BHV-1) in Abnormal Cervical<br />

Mucous Discharge <strong>and</strong> Fertility in Cows <strong>and</strong> Heifers<br />

S. Yavru 1 , M. Kale 2 , A. Ayhan 3 , O. Bulut 4 , MS. Gulay 5 ,<br />

O. Yapkc 4 , F. Pehlivanoglu 6<br />

1<br />

University of Selcuk, Faculty of Veterinary Medicine, Virology,<br />

Konya, Turkey<br />

1<br />

University of Mehmet Akif Ersoy, Faculty of Vet. Med., Virology,<br />

Burdur, Turkey<br />

3<br />

University of Mehmet Akif Ersoy, Faculty of Vet. Med., Department<br />

of Artificial Insemination, Burdur, Turkey<br />

4<br />

University of Selcuk, Faculty of Veterinary Medicine, Virology,<br />

Konya, Turkey<br />

5<br />

University of Mehmet Akif Ersoy, Faculty of Vet. Med., Department<br />

of Physiology, Burdur, Turkey<br />

6<br />

University of Mehmet Akif Ersoy, Faculty of Vet. Med., Department<br />

of Veterinary Microbiology, Burdur, Turkey<br />

The purpose of the present study was to describe whether Bovine Viral<br />

Diarrhea Virus (BVDV) or Bovine Herpesvirus-1 (BHV-1) in<br />

abnormal cervical mucous discharge (A-CMD) has effects on fertility<br />

of cows <strong>and</strong> heifers. For this purpose, after visual examination of CMD<br />

of the animals in estrus (n=222), animals having clear discharges (68<br />

cows, 38 heifers) with normal viscosity <strong>and</strong> without bad odor were<br />

grouped as normal cervical mucous discharge (N-CMD) group. The

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