Oral and Poster Abstracts

Institute of Physiology and Sanocreatology, Chisinau, Moldova
Spermogram is the wide used laboratory’s method for researching
the state of bull’s reproductive system. Sperm or ejaculate is the
mixture of excretion of men’s genitals secretion products (testicle
and their appendages, prostate glands, seminal vesicles,
bulbourethral (Cowper’s) glands, urethra) and consists of the liquid
from seminal vesicles, liquid from prostate and spermatozoon.
Collected sperm is putted into test-tube for the centrifugation during
20 min. with 10 000 r/min. Plasma of sperm in quantity of 1 ml is
putted into the other test-tube and deposited with the help of
sulfosalicylic acid (6%). The liquid is mixed and is leaved for 1 hour
in a refrigerator and after is centrifuged during 30min with 3000
r/min. The sample has to be stored in place with low temperature.
Acid from this liquid is evaporated with help of rotary evaporator
with temperature of 40 °C, adding distilled water periodically till
time when pH will be 2.2. This process has to be done till the sample
will be dry out. It is possible to analyze the same without preliminary
drying out. Sperm is found in the sediment of the sample (3-30mg).
It is influenced with 1 ml of sulfosalicylic acid (6%), after is
centrifuged during 30min with 3000 r/min. Acid from this liquid is
evaporated with help of rotary evaporator with temperature of 40 ºC,
adding distilled water periodically till time when pH will be 2.2.
Sediment dissolves in starts lithium buffer (1ml, pH 2.2). Sample is
stored in refrigerator. The analyze is carried out in standard regime
of free amino acid’s determination using lithium buffer (pH 2,90;
2,95; 3,20; 3,80 and 5,00) with the speed of flow 12,0 ml/hour. The
obtained data were statistically analyzed using Students t-criteria
method (Mercurieva, 1976). It was revealed that sum of bull’s free
amino acids in sperm liquid was 1835.4±579.77 micromole/100mg
but in sperm it was 35.8±17.53micromole/100mg. Analyzing
obtained data we can make a conclusion that it is corresponding to
the existing standards and that free amino acids contains in sperm
liquid more than in sperm.
931 Definition of Quality Sperm of the Bulls Frozen with High
Effective Environments at Use of Different Technological
Modes
V. Buzan, Gh. Boronciuc, V. Buzan, I. Mereuta
Institute of Physiology and Sanocreatology, Chisinau, Moldova
Efficiency of freezing biological objects is defined by components
of synthetic environments and modes of freezing. These two
directions are important and require studying at updating or
improvements of technologies of long storage of sperm of
agricultural animals. In result the lead experiences it has been proved
that one of the best-investigated environments is medium lactosesorbite-glycerin-yolk.
One of the important factors at freezing sperm
is the stage of cooling. Therefore, in one special experience
influence of the period of cooling and time of filling of sperm has
been investigated. For research sperm of bulls of breed Baltata cu
negru (Holstein) farms containing in conditions was used. Mobility,
life expectancy and an absolute index have been appreciated with the
accepted methods. It was used sperm of the bull with mobility of not
less than 7.0 points and concentration of 0.7 for experiences. With
medium was 1:5. At crioconservations sperms of the bull in
polymeric LSTGG environment was used. All experiences repeated
not less than 5 times. For performance of total problems, experiences
were carried out under below specified scheme. In experience,
influence of duration of cooling of sperm is investigated at 2-40.
Besides it, time of cooling of sperm in a refrigerator before filling
and after that is investigated. Performance of the given circuit
promotes definition of optimum time of freezing of sperm of the bull
and at what height from a surface of liquid nitrogen this
technological operation demands for realization. Proceeding from
experiences at use of LSTGG environment for freezing sperm of
bulls in polymeric the following circuit is offered: 1. Filling
polymeric sperm at once after it. 2. Cooling a seed material during 3
hours. Such simplified mode allows receiving quality of the frozen
sperm, which corresponds to technology requirements. Other series
of researches has been directed for research of the new environment
for freezing sperm of bulls. As a result of the lead researches, it is
possible to draw conclusions: 1. Cooling sperm of bulls is necessary
to carry out during 3 hours at temperature 2-4. 2. It is possible to
dilute sperms, to put it in polymeric without its adaptation to new
conditions.
212 XXV. Jubilee World Buiatrics Congress 2008
932 Effect of Lineage of Oocyte Donors on the Efficiency of OPU
in Nellore Cows
A. Martins Jr 1 , R. Calegari 1 , C. Silva 1 , A. Crespilho 2
1 Sao Paulo State University - UNESP, Clinic, Surgery and Animal
Reproduction, Aracatuba SP, Brazil
2 Sao Paulo State University - UNESP, Animal Reproduction and
Veterinary Radiology, Botucatu-SP, Brazil
It is well-known that oocyte donors can influence the number of
recovered oocytes by ultrasound-guided follicular aspiration.
Thereby, this study was designed to assess the effect of lineage of
donors on the number of cumulus oocyte-complexes (COCs)
harvested by ovum pick up (OPU). Fifty-six Nellore (Bos indicus)
cows derived from two distinct lineages of bulls, named Karvadi (K)
and Taj Mahal (T) were used. The animals were assigned to four
groups, according to the maternal (m) or paternal (p) lineage, as
follows: Kp-Km (n=15), Kp-Tm (n=11), Tp-Km (n=21), and Tp-Tm
(n= 9). The experiment was conducted over two consecutive years.
All visible follicles were punctured using a real time B-mode
scanner (Aloka SSD-500, Japan) and a transvaginal convex
transducer (5 MHz) to which a needle guide and a suction pump
were connected. Retrieved COCs were evaluated and classified into
seven categories (I-VII), graded from the best (I) to the worst (VII;
degenerated) quality, based on the cumulus cells investment and
cytoplasmic appearance. The data were transformed into log (x+1)
before statistical analysis (ANOVA and Tukey’s test) with P