Oral and Poster Abstracts

and HCO 3 level. The concentration of K ions holds in reference values.
The concentration of Na ions was various, but still was on proper
levels.
Conclusions: The DCAB levels of diets oscillated between +66 and
+228 meq/kg DM and were lower then optimal. Obtained BE indices
were higher than reference value, that stated about alkalosis
occurrence.
Key words: dietary cation-anion balance, feeds, dairy cows
514 GH Serum Values in Dairy Cows with Clinical Primary
Ketosis
A. Cuomo 1 , L. Scaletta 1 , G. Bertoni 2 , L. Esposito 3
1
Faculty of Veterinary Medicine, Dipartimento di Scienze Cliniche
Veterinarie, Teramo, Italy
2
University Cattolica Sacro Cuore, Istituto di Zootecnica, Piacenza,
Italy
3
Faculty of Veterinary Medicine, Dip. Scienze Zootecniche ed Isp.
Alimenti, Napoli, Italy
The objective of the present study was to investigate the GH values in
dairy cows with spontaneous clinical ketosis. Eight ketotic Holstein
Fresian cows (KT group) within the sixty days after parturition, with ßhydroxybutyrate
(BHBA) values higher than 1.2 mmol/L and
nonesterified fatty acid higher than 0.6 mmol/L, were evaluated for
metabolic profile, plus RIA for insulin and GH. Before sampling the
clinical diagnosis of ketosis was confirmed by portable dry analyzer
Medisense-Optium Abbott. A control group of six normal no lactating
cows (CT group) was evaluated in the same way. Any subject was treated
by drugs before sampling and the samples were collected at 09.00 a.m. in
both groups. Statistical analysis was performed respectively in the
groups and between the groups, by software GraphPad Prism 5. In the
group KT mean value was respectively for GH 2.473 ab ng/ml (SD
1.038), insulinemia, 4.570 bcA µU/ml (SD 2.101), glycaemia 46.00 bC
mg/dl and (SD 7.746), BHBA 2.213 ab mmol/L (SD1.606) and NEFA
1.796 ac mmol/L (SD 0.5299). In the CT group mean value was
respectively for GH 2.162 a ng/ml (SD 0.9184) insulinemia 8.445 bB
µU/ml (SD 1.082) glycaemia 71.50 ABC mg/dl (SD 8.264) BHBA
0.283333 a mmol/L (SD 0.075277) and NEFA 0.1904 a µmol/L (SD
0.1692). All the results are in accordance with the literature except the
GH in KT group. Usually in cow 60 days post-parturition the GH value
ranges around 5-10 ng/ml, significantly different respect our data in
ketotic cows for the same period of lactation. Statistical analysis shows
between the groups significance for insulinemia, glycaemia, BHBA and
NEFA values as expected, at difference of GH that is not significant. No
correlation has been observed between NEFA and GH values in the KT
group, at difference of positive correlation described in literature for
ketosis-free lactating cow. We hypothesize, that low GH concentration in
dairy cow affected by primary ketosis, could have a protective effects
against more pronounced fats mobilization during NEB.
Key words: cow, ketosis, GH
515 Romifidine as Treatment for Primitive Ketosis in Dairy Cow
A. Cuomo 1 , L. Scaletta 1 , G. Bertoni 2 , L. Esposito 3
1
Faculty of Veterinary Medicine, Scienze Cliniche Veterinarie,
Teramo, Italy
2
University Cattolica Sacro Cuore, Istituto di Zootecnica, Piacenza,
Italy
3
Faculty of Veterinary Medicine, Dip. Scienze Zootecniche ed Isp.
Alimenti, Napoli, Italy
Romifidine (RMF) is an a-2 agonist utilized like analgesic-sedative drug
in dog and horse, and rarely in cow. The a-2 agonist drugs induce a
transient anabolic effect with hyperglycaemia, biphasic hypohyperinsulinemia
and a rise of GH concentration. The objective of this
work was to evaluate the effectiveness of Romifidine in the treatment of
ketosis and its metabolic effect in dairy cows. Eight dairy cows affected
by clinical ketosis (KT group), with ß-hydroxybutyrate (BHBA) values
higher than 1,2 mmol/L and nonesterified fatty acid (NEFA) higher than
0.6 mmol/L, were included in the group. All the animal displayed the
syndrome in the sixty days after parturition and do not received treatment
before our study. Six not lactating cows with normal BHBA values were
enrolled in the control group (CT). Both groups were evaluated, for
metabolic profile plus GH and insulin, to study the RMF effects in a
negative energy balance mode (NEB) like group KT and in a positive
energy balance (PEB) like group CT. In KT group RMF was injected at
34 XXV. Jubilee World Buiatrics Congress 2008
09.00 a.m. for 5 days at dose of 7.5 ϵg/Kg/i.m, after collection of basal
blood sample and evaluation of levels of BHBA, by portable dry analyzer
Medisense-Optum Abbott. Adjunctive sample was collected at day 1 after
2.5 hours from RMF injection. In CT group the samples were collected
before injection and at 1; 1.5; 2; 2.5;3; 24 hours after it to evaluate a
complete daily curve. Each sample was processed for metabolic profile
plus insulin and GH and data were statistically processed. The sedative
RMF at 7.5 µg/Kg/i.m. induces a significant increase in GH and
glycaemia values and a biphasic hypo-hyperinsulinemia in both groups,
without any sedation effect. GH mean value shows significant differences
between KT and CT groups, before and after 2.5 hour RMF injection
respectively of 2.473 ab (SD 1,038) and 9.225 A ng/ml (SD 8.774) in KT
and 2.162 a (SD 0.9184) and 3.022 a ng/ml (SD 1.206). In the group KT
BHBA mean value falls down at 1 mmol/L (SD 0.7838) and food intake
normalizes within 5 days. RMF is effective as unique treatment for
primitive ketosis in dairy cow. We hypothesize, to explain this action, a
role of described hyperglycaemia and transient hypo-hyperinsulinemia,
plus the normalization of GH values, without the predictable increase
values of NEFA, wich is typical for GH action in NEB cow. For this
reason we could hypothesize some additionally, direct actions of RMF not
correlated with GH activity.
Key words: cow, ketosis, G˙, romifidine
516 Mycotoxin Screening in Healthy Cows and Cows with
Abomasal Displacement
A. Alkaassem 1 , M. Fürll 1 , S. Dänicke 2
1 University of Leipzig, Medizinische Tierklinik, Leipzig, Germany
2 Federal Agricultural Research Center (FAL), Animal Nutrition,
Braunschweig, Germany
Objectives: The aim of this study was a) to examine samples of fodder,
blood, milk and bile for concentration of deoxynivalenol (DON),
Zearalenone (ZON) and their metabolites de-epoxy-DON, a-zearalenol
(ZOL), ß-ZOL, zearalanon (ZAN), a-Zearalanol (ZAL) as well as ß-
ZAL, b) to study possible relations between the toxin arrears and
diseases.
Material and methods: We checked 61 ill cows with abomasal
displacement and accompanying diseases as well as 13 healthy HF
cows.
Results: 77.8% Patients without mycotoxins were cured. From those
with mycotoxins 18 (72%) were cured without clinical reactions; 7 had
to be euthanized due to severe accompanying diseases and severe
haematological or clinical chemical deviations in the blood being
typical for abomasal displacement. We do not observed specific
clinical mycotoxins effects on health. ZON/ZOL associated changes in
the ovaries and uterus could not be macroscopically determined. The
transabdominale collection of bile by aspiration can be easily
performed in sick cows. In healthy cows mycotoxins were not found. In
the slightly contaminated fodder samples the mycotoxin concentration
were 0.161 g DON /kg (0.086-0.191) and 6.35 µg/ ZON kg (4.88-7.85).
None milk samples contained mycotoxins. DON (0,002 µg/ml) was
found in one of 61 serum samples, de-epoxy-DON in four samples
(8%). In bile de-epoxy-DON (37.6 µg/ml) was found only once. 39% of
the examined bile samples were contaminated with ZON and/or its
metabolites (ZON 9.85 ng/g [8.10-16.33], b-ZOL: 59.9 ng/g [5-78] and
ß-ZOL: 37.6 ng/g. In all cases concentrations were within the
physiological range. The metabolic parameters protein, albumin, urea,
kreatinin and TEAC did not show differences in cases with or without
mycotoxins. Mg, Ca, Na, K, Cl, pH, PCO2, bilirubin, glucose, FFA and
cholesterol concentrations were not in physiological ranges in all cases
independed on mycotoxins. The haemogram of all patients with or
without mycotoxin did not show deviations. AP, GGT and CK
activities of all patients were within the normal ranges. However mean
AST and GLDH activities of all mycotoxin positive patients were
slightly increased compared to mycotoxin negative cows.
Conclusions: We concluded that no specific clinical, haematology or
clinical chemical changes for mycotoxin influences (DON, ZON) could
be determined.
517 Strong Electrolytes (Sodium, Potassium, Chloride) Determine
Hydrogen Ion and Bicarbonate Concentrations in Urine of
Ruminants
H. Staempfli 1 , P. Constable 2
1
Ontario Veterinary College, Department of Clinical Studies,
Guelph, Canada