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Oral and Poster Abstracts

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2 Veterinary Medicine Faculty, Dept. Physiological, Biochemical <strong>and</strong><br />

Cellular Sciences, Sassari, Italy<br />

Objectives of study: Energy metabolism is a key factor supporting cell<br />

functions <strong>and</strong> male gamete require exceptionally more ATP than other<br />

cell types in order to sustain its high queue activity. The aim of this<br />

study was to evaluate the relationhips between ATP concentration,<br />

viability <strong>and</strong> motility in fresh <strong>and</strong> cryopreserved-thawed spermatozoa<br />

during the breeding season.<br />

Materials <strong>and</strong> Methods: During the breeding season, semen was<br />

collected by artificial vagina from three bucks originating from native<br />

stocks in Ogliastra area of Sardinia. Semen was cryopreserved in 4%<br />

glycerol, cooled to 4 °C, frozen in 0.25 ml pellets on dry ice <strong>and</strong> then<br />

plunged into liquid nitrogen. Fresh <strong>and</strong> frozen/thawed semen samples<br />

from the three males were evaluated for viability by PI fluorescent<br />

staining <strong>and</strong> for progressive motility by Sperm Class Analyzer (v.<br />

3.2.0. Microptics S.L., Barcelona, Spain), while ATP levels were<br />

measured spectrophotometrically at 340 nm using NADH-linked<br />

enzyme-coupled assays (H.U. Bergmeyer, Principles of enzymatic<br />

analysis, Academic Press, New York ,1974) modified for being<br />

adapted to the our system. Data were analysed using Statgraphics<br />

Centurion XV (Statpoint, inc).<br />

Results: Our data show that both ATP, progressive motility <strong>and</strong><br />

viability do not differ between subjects nor between ejaculates in both<br />

fresh <strong>and</strong> frozen semen (Anova; P

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