Oral and Poster Abstracts

organs were identified. However, the results varied a lot within individual
groups. Mean values of Cu concentration in individual groups ranged as
follows (tissues are ordered in a descending line): liver (9.77-12.95 mg/kg
of fresh tissue), heart (3.90-4.41 mg/kg), kidney (2.49-2.92 mg/kg), lungs
(2.45-2.66 mg/kg), tongue (1.81-1.94 mg/kg), diaphragm (1.39-1.61
mg/kg), pancreas (1.25-1.56 mg/kg), spleen (1.24-1.52 mg/kg), thigh
musculature (0.85-1.03 mg/kg) and neck musculature (0.77-0.87 mg/kg).
On the basis of our results Cu supplementation with concurrent
administration of Zn, Mn and Se in higher doses does not cause different
concentrations of Cu in individual tissues and organs of the goats.
The work was realized with the support of MSM Project No.
6215712403.
Key words: microelements, liver, kidney, heart, lungs
495 Evaluation of Copper Status in Dairy Cows Living in Region
Irrigated with Potato Utility Refuses
M. Kleczkowski, W. Kluci_ski, T. Jakubowski, K. Dembele
Warsaw University of Life Sciences, Faculty of Veterinary Medicine,
Clinical Sciences, Warsaw, Poland
Copper status (CS) can be determined by 5 blood parameters: total
plasma copper (Cu) zinc (Zn), molybdenum (Mo) as well as
ceruloplasmin (Cp), and superoxide dismutase (SOD) activities. The
aim of experiment was to evaluate CS from the region irrigated with
potato utility refuses. The experiment lasted for 6 months, and it
comprised 45 cows of the Black-White breed, at the 3 - 6 years. Cows
were chosen from the 3 districts in the valley of Narew River, each
group (1, 2 and 3) with a different irrigation method. Cows, from 1 and
2- experimental groups, inhabited areas irrigated with utility refuses
from potato industry company. Area inhabited with cows from 1 group
was irrigated with utility refuses by using a flow method. Area
inhabited with cows from 2 group was irrigated with utility refuses by
using a sprinkle method. Cows from 3 group were control. Mineral
analysis of utility refuses had been made before experiment started.
Blood samples from all cows were collected 3 times: 1- one week
before calving, two-three days after calving, and 3- six weeks after
calving. Cow blood parameters that were determined are: Cu, Zn, Mo
concentration, and Cp, SOD activity. Average value of particular
parameters for cows from group 1 was: Cu- 8.99 µmol/ dm 3 , Mo -0.24
µmol/ dm 3 , Zn- 11.46 µmol/ dm 3 , SOD- 70.11 U/gHb, Cp- 1.37 µmol/
dm 3 , for cows from group 2 was: Cu- 7.23 µmol/ dm 3 , Mo- 0.21 µmol/
dm 3 , Zn- 9.82 µmol/ dm 3 SOD- 54.22 U/gHb, Cp- 0.89 µmol/ dm 3 , for
cows from control group 3 was: Cu- 12.07 µmol/ dm 3 , Mo- 0.26 µmol/
dm 3 , Zn- 14.55 µmol/ dm 3 , SOD- 81.36 U/gHb, Cp- 1.66 µmol/ dm 3 .
The analysis of results showed that nutrition of dairy cows produce a
decrease of copper status and Cu-dependent antioxidant activity in
blood of cows.
Key words: cows, copper, antioxidants, utility refuses
496 Diurnal Variation of Acid Base Status in Cows Fed Different
Anionic Salts Once a Day Only
C. Gelfert 1 , A. Löptien 2 , N. Montag 2 , R. Staufenbiel 2
1
University of Veterianary Medicine, Clinic for ruminants, Vienna,
Austria
2
Faculty of Veterinary Medicine, Freie Universitaet Berlin, Clinic for
Ruminants and Pigs, Berlin, Germany
Eleven non-lactating, non-pregnant, Holstein-Friesian-Crossbreed
cows received 2,000 meq of either calcium chloride (CaCl 2) or calcium
sulphate (CaSO 4) once a day for a period of one week. At day seven,
blood and urine samples were taken every four hours starting at 6
o’clock before feeding and ending the same time the next day. Before
feeding, urine analysis of the CaCl 2-cows showed a compensated
acidosis due to an reduced pH and low levels of net acid-base excretion
(NABE). Feeding AS changed acid-base status (ABS) noticeably in
both groups over time. All cows developed a strong metabolic acidosis.
The strongest acid load was visible 18 hours after the morning feeding.
Changes were greater in CaCl 2-cows and NABE differed significantly
(p=0.037) between both anionic salt groups. Nadir of NABE was -
41mmol7l in CaCl 2-cows and +4mmol/l in the CaSO 4-cows. The
fractional clearance of calcium was higher (p=0.027) in the CaCl 2cows
due to a higher calcium excretion via the kidneys. Serum calcium
concentration did not show significant differences between both groups
(p>0.05) but changed significantly during the day (p