Oral and Poster Abstracts

1 Islamic Azad University of Shahrekord, College of Veterinary
Medicine, Shahrekord, Iran
2 Facutly of Veterinary Medicine, University of Tehran, Department
of Clinical Science, Tehran, Iran
This study performed in order to determine of rotaviral and coronaviral
diarrhea prevalance in newborn calves in Iran, in the period of 12 months
(2004-2005). Antigen detection ELISA kit was used for determination of
rotaviral antigen and Coronaviral antigen in fecal samples. Among 200
fecal samples 37 of them (18.5 %) were positive to Rotavirus and all of
them were negative with coronavirus infection. During different seasons,
the lowest rate of infection was in summer (6%) and high prevalence was
in winter (48%). Frequency of infection rate of male calves was 14.28 %
and among female ones was 20.13%. In dairy farms with a proper
hygienic management, frequency of Rotaviral diarrhea was 5.19% and
among the calves of the farms without a proper health management this
frequency was about 63.04%. In different age groups, the least Rotaviral
frequency was among 1-7 days age group and it was reported to be 3.17%
and the most one was among the 15-21 days age group with 66.66% of
infection rate. Statistical analysis of the results showed a significant
association between infection, season and health management in the farm.
Infectious frequency among the calves with 15-21 days of ages had a
significant statistical difference with calves in other age groups. But no
linear statistical correlation was founded between increase in infection
rate and age of the calves.
716 Seroepidemiological Study of BVD, IBR, RSV and PI3 in
Morocco
Z. Zouagui, S. Alali, A. Aiyar
Institut Agronomique et Vétérinaire Hassan II, Morocco, Pathologie
Médicale et Chirurgicale des Ruminants, Rabat, Morocco
The objective of this study is to assess the seroprevalence of four
economically important viral infections among Moroccan cattle herds.
Theses infections are bovine virus diarrhoea (BVD), infectious bovine
rhinotracheitis (IBR), bovine respiratory syncytial virus (RSV) and
parainfluenza-3 virus (PI3) infections. Our work aimed also to reveal
possible relationships between the prevalence of these viruses and
some epidemiological factors. For this purpose, a large
epidemiological investigation has been undertaken in six regions,
known to be important regarding cattle rearing (Doukkala, Haouz,
Gharb, Moulouya, Tadla and Souss-Massa). 1609 blood samples,
collected from non vaccinated cattle, have been analyzed by the ELISA
method to asses their status concerning the four viruses. The
serological results showed that the general antibody prevalence was
39%, 23%, 79% and 68% for BVD, IBR, RSV and PI3, respectively.
However, regional variations of the four infections have been recorded.
Moreover, the majority of the investigated farms were significantly
affected by the mentioned viruses. The survey revealed also that cattle
born and raised in Morocco were more affected than those imported
since several years. On the other hand, IBR prevalence has been
partially influenced by the method of service used.
Key words: BVD, IBR, RSV, PI3, Morocco
717 Polimerase Chain Reaction Standardization for Semen, Urine
and Organs of Rams Experimentally Inoculated with B. ovis
C. Nozaki, N. Lira, J. Megid
UNESP- School of Veterinary Medicine and Animal Sciences, Higiene
Veterinária e Saúde Pública, Botucatu-SP, Brazil
The objective of the study was to adapt and evaluate PCR compared
with microbiological culture in the detection of B. ovis in semen,
urine and organs of experimentally inoculated rams. Thirty one rams,
1-2 years old, were challenged by intrapreputial and conjuntival route
with a suspension containing 2x109 CFU/mL of B. ovis. All animals
inoculated were weekly submitted to clinical evaluation, as well as
collection of blood for serological diagnosis, and collection of semen
and urine for culture and PCR. From the 31 experimentally inoculated
animals, PCR detected bacterial DNA in the semen of 24 animals,
whereas culture showed the agent in 13 rams. PCR showed greater
sensitivity (77%) than culture (42%). In urine samples, sensitivity of
the techniques was not significantly different (48% for culture and
58% for PCR). PCR detected the presence of the agent in 25% of the
organs tested while culture detected it in only 2% of the samples.
Although no statistical difference was observed between the
106 XXV. Jubilee World Buiatrics Congress 2008
techniques, results suggest that PCR should be used instead of
microbiological culture.
Key words: Brucella ovis, PCR, culture, rams, epididymitis
718 Serological Profile of Rams Experimentally Inoculated with
Brucella ovis in the Different Phases of the Illness:
1 Correlation with Clinical Signals 2 Evaluation of Agar Gel
Immunodifusion Test and Serum Neutralization Test
C. Nozaki 1 , N. Lira 1 , O. Augusto Filho 1 , H. Azevedo 2 , L. Rodello 2 ,
S. Bicudo 2 , J. Megid 1
1
Universidade Estadual Paulista, Higiene Veterinária e Saúde
Pública, Botucatu-SP, Brazil
2
Universidade Estadual Paulista, Reproduction Animal, Botucatu-
SP, Brazil
The present research had as objective to determine the serological
profile after experimental inoculation of rams with B. ovis using the
Agar Gel Immunodifusion test and Serum aglutination test (SAR)
using the commercial kit D-TecCB. The results were correlated to the
reproductive clinical signals, evaluating the phases of higher
effectiveness of the serological tests and the method of clinical
evaluation in the different phases of the experimental illness. Thirty
one rams, 1-2 years old, were challenged by intrapreputial and
conjuntival route with a suspension containing 2x10 9 CFU/mL of B.
ovis REO 198, simultaneously. After inoculation, animals were weekly
submitted to clinical evaluation and blood collection for serological
analysis. SAR showed a decreasing percentage of positive results, from
80% to 11%, from the second to the seventh week after inoculation.
IDGA showed increasing percentages of positive results, from 45% to
63%, from the first to the third week, and then a decrease to 4% in the
11 th week. Fluctuation in titers was observed in both tests. Clinical
changes began in the first week after inoculation and were associated
with positive serology in the acute phase of the disease. IDGA
demonstrated variable sensitivity in the different phases of the disease,
showing positive animals in the chronic phase, and was preferred in
routine diagnosis. Negative serological results associated to clinical
alterations were characteristics of the chronic phase of the disease
demonstrating the inefficacy of serology in this phase.
Key words: agar gel immunodifusion test, serum aglutination test,
sheep brucellosis, clinical signs, serology, experimental inoculation
719 Seroprevalence of Six Serovars of Leptospira in Human and
Bovine from Toca, Boyacá (Colombia)
J. López, M. Pulido, R. Andrade, A. Caycedo
Universidad Pedagógica y Tecnológica de Colombia, Grupo de
investigación en medicina veterinaria y zootecnia, Tunja, Colombia
Objectives: Determining the presence of serovars of Leptospira in
human and bovine by means of the microscopic agglutination test
(MAT), and; establishing the probable source of serovars as much in
the humans as in the bovine ones.
Materials and methods: Location: six properties milkmaids from the
rural area of Toca, Boyacá (cold climate). 36 mature people (26 women
and 11 men) donated 4 ml of blood of the radial vein. 116 samples from
bovine were collected, corresponding to 8 ml of blood from the caudal
vein. Samples were centrifuged (3.600 rpm during five minutes) and
the resulting serum froze to -20° C until its analysis with MAT.
Results: Serums from people had a positivity of 29.7% (11 of 36);
being from Canicola 10,8% (4 of 36); Icterohaemorragiae, Pomona and
Automnalis 5,4% each one, (2 of 36 people each serovar) Hardjo 2,7%
(1 of 36). The serovar Sejroë was seronegative in human. In bovine
there was a 48,1% of positivity (64 of 133); being from
Icterohemorragiae 11,3% (15 of 133), Pomona 13.5% (18 of 133),
Hardjo 6.0% (8 of 133), Canicola 8.3% (11 of 133), Sejroë 6.8% (1 of
133); Automnalis 8.3% (11 of 133).
Conclusions: Prevalence obtained of different servars of Leptospira
interrogans is adjusted to data obtained by other investigators in
Colombia. The results don’t offer a precise interpretation about the
origin of the infection with different stumps of Leptospira interrogans
so much in human as in bovine. There was not statistical significance in
any association, in spite of this, infection probabilities exist between
human and bovine: For serovar icterohaemorragiae 2,22 times in
bovine that in human; serovar pomona (2,73 times more), hardjo (2,3
times more) and automnalis (1,57 times more). This would be due to
the biggest contact among bovine and elements contaminated by urine