Oral and Poster Abstracts

not influence the concentration of Zn in milk, but significantly influenced
the Zn concentration in blood serum. The efficiency of different organic
and inorganic forms of zinc was almost the same.
The work was realized with the support of MSM Project No.
6215712403.
Key words: microelements, serum, Zn
473 The Evaluation of Coagulation Profiles in Cows with
Abomasal Displacement
P. Sobiech, Z. Kuleta, J. Radwinska
University of Warmia and Mazury, Department of Internal Medicine,
Olsztyn, Poland
Abomasal displacement occurs in high-yelding cows during early
lactation. The most common form of this disease is left abomasal
displacement, in which the abomasum is trapped between the rumen
and the left abdominal wall. The purpose of the study was to evaluate
coagulation profile in cows with this disorder.
Material and methods: Tests were carried out on 20 cows of H-F breed
(Holstein Friesian) divided into two groups: the first group 10 cows with
left abomasal displacement, the second 10 healthy cows. Diagnosis of
abomasal displacement was confirmed by abdominal ausculopercussion,
rectal examination and verified by operation. Laboratory tests included
marking, using CoA Chrom 3003 BIO-KSEL Apparatus, such
parameters as: prothrombin time (PT), fibrinogen concentration (FIB),
activated partial thromboplastin time (APTT), thrombin time (TT), level
of antithrombin III (AT III) and level of D-Dimer.
Results: Mean values of determined parameters amounted in cattle with
abomasal displacement: PT 20,43 sec., FIB 7,99 g/l, APTT 47,31 sec., TT
26,63 sec., AT III 81,33 %, D-Dim 849,33 µg/l. In healthy cows these
parameters reached values as follows: PT- 25,12 sec, FIB 4,15 g/l, APTT
33,71 sec., TT 24,84 sec., AT III 111,12 %, D-Dim 286,15 µg/l.
Discussion: In this study it was found that cattle with left abomasal
displacement had significantly higher fibrinogen level, APTT time and
extremely high D-dimer level together with significantly lower AT III
level. Obtained results indicated, that ill cows showed abnormal
coagulation profile which reflect the occurrence of disseminated
intravascular coagulation syndrome (DIC). The laboratory diagnosis of
DIC is based on prolonged APTT, PT, hypofibrinogenaemia, decreased
concentration of coagulation factors, increased D-Dim level and
decreased AT III activity. A diagnosis of DIC is concerning when at
least three tests in coagulation profile are abnormal. According with
literature data, DIC is very common complication in cattle with
abomasal displacement, especially with right displacement, which is
usually accompanied by a degree of volvulus.
Conclusion: During left abomasal displacement in cows occurred
disseminated intravascular coagulation syndrome with different level
of coagulation abnormalities.
474 Bread Leftovers Utilization in Cattle Rations in Tadla Area
A. Ilham, M. Mazouz, S. Alali
Institut Agronomique et Vétérinaire Hassan II, Morocco, Pathologie
Médicale et Chirurgicale des Ruminants, Rabat, Morocco
The present study aimed to evaluate the importance of bread leftovers
utilization by cattle producers in Tadla area in Morocco and to assess their
nutritive value as well as their effects on dairy cow performances and
diseases incidence. A follow up study was conducted during 3 months to
compare the performances and the diseases occurrence in a sample
consisting of 12 dairy farms among which 6 farms totalizing 51 cows used
bread leftovers all the year around (F 1) and the 6 others composed of 53
cows that have never used them (F 2). The main results obtained are as
follows:
- Among all cattle producers in Tadla area, 35.8 % supplemented the
rations with bread leftovers from which 16.4 % used them all the year
around and 19.4 % only during periods of feed shortage.
- The total quantity of bread leftovers used per year in Tadla area was
estimated to about 5230 metric tons.
- The incorporation rate of bread leftovers in the concentrate portion of
cattle rations varied from 20 to 100 % with an average rate of 30 %. The
daily quantities used varied between 2 and 6 kg per cow with an
average of 2.3 kg.
- The composition per kg of bread leftovers was: 88.1% DM, 0.9% CF,
11.9% CP and 3.6% Ash. The nutritive value was: 1.28 UFL (French
dairy feed unit) / kg DM and 104.4g DCP / kg DM.
- There was no significant difference between F 1 and F 2 regarding milk
production and body live weight. However, the body condition score was
in favour of cows of F 1 and the milk fat test was 1.27 % higher in F 2.
- The incidence of health problems was higher in F 1 compared to F 2.
Thus, abortion, acidosis and bloat rates were 15.7 vs. 7.4 %, 9.8 vs. 3.7
% and 11.8% vs. 5% respectively in F 1 and F 2.65
Key words: bread leftovers, nutritive value, dairy cattle, performances,
health problems
475 Influence of Diarrhoea on Chosen Indicators of the Blood
Coagulation System at Dairy Cattle
J. Radwinska, Z. Kuleta, P. Sobiech
University of Warmia and Mazury, Department of Internal Medicine,
Olsztyn, Poland
Objectives of study: In cattle breeding important is prevention and
treatment of diarrhoeas. There are many factors causing diarrhoea. The
aim research was evaluation of diarrhoea influence on chosen
indicators of the blood clotting system at the dairy cattle.
Material and methods: Research was passed on 2 groups of dairy
cows. In group I were 16 cows with diarrhoea. Group II - 20 healthy
cows (control group). The blood were taken from jugular vein.
Coagulation factors were marked in blood plasma. To the laboratory
analysis was used photometer Coag-Chorm 3003 (Bio-Ksel). During
research were marked: prothrombin time(PT), thrombin time(TT),
activated partial thromboplastin time(kaolin-kephalin time, APTT),
fibrinogen, antithrombinIII(ATIII) and D-dimer. During research was
not identify primary reason of diarrhoea.
Results: There were changes in times length between ill and healthy
cows. PT at cows with diarrhoea was longer than in healthy. There was
an APTT extension (mean APTT at group I - 37,675s and group II -
34,24s). In group I a mean TT was 31,39s in group II 26,44s.
Fibrinogen concentration at ill cattle increased (6,61g/l) to healthy
cattle (5,24g/l). Mean value of D-dimer in group I and II differed about
103 µg/l. ATIII in each groups were: group I 108,7% and group II
107,7%. PT describes exogenous tract and APTT characterize
endogenous track of the blood clotting. The extension of PT is
observed in deficiency of II, V, VII, X factors. All mentioned factors
are dependent on vitamin K. A length of APTT is connected with II, V,
X, VIII, IX, X, XI, XII factors. The PT prolongation might be
connected with vitamin K malabsorption. This can be induced by
changed bacterial flora in intestines (production vitamin K) or handicap
absorption from alimentary canal (damages at intestine epithelium
during infections). An increased D-dimer concentration (product of
digestion stabilized fibrin) and TT prolongation (measures activity of
fibrinogen) at the group I were connected with long PT and APTT. This
can be an evidence of disseminated intravascular coagulation (DIC) in
cow organism. Increased concentration of fibrinogen is DIC laboratory
symptom. The activity of ATIII did not differ at sick and health cattle.
Conclusions: Diseases with diarrhoea have influence on whole
organism also on blood coagulation system. Abnormality in this system
can lead to serious problems like DIC.
476 Diagnostic Use of Pooled Metabolic Profiles in Czech Dairy
Herds
J. Davidek 1 , R. Van Saun 2
1 Practitioner, Krasna Hora nad Vltavou, Czech Republic
2 Penn State University, Veterinary & Biomedical Sciences,
University Park, United States
Study objective was to compare blood analyte concentrations collected
at different periods relative to calving to determine diagnostic value in
herd-based assessment of transition cow problems. In the course of
clinical investigations on 37 dairy herds presented for various
periparturient diseases or routine monitoring, blood was sampled from
3 to 7 mature cows within defined time periods to run a pooled sample
metabolic profile. Time periods were defined as (days relative to
calving): Early Dry (>30 d prior), Close-up (