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Online proceedings - EDA Publishing Association

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11-13 May 2011, Aix-en-Provence, France<br />

<br />

Particles occupance(%)<br />

100<br />

90<br />

80<br />

70<br />

60<br />

50<br />

40<br />

30<br />

The sample of latex particles: 6 μL; 10 6 particles/mL<br />

20 μm microwells<br />

30 μm microwells<br />

Figure 1: Schematic diagram of the microfluidic chip for single-particle-based<br />

microarray.<br />

20<br />

10<br />

5 10 15 20 25<br />

Applied voltage (volts)<br />

Figure 4: Particle occupancy of latex particles in the microwells of 20-μm and<br />

30-μm diameter for different applied voltages.<br />

Particle occupance (%)<br />

100<br />

90<br />

80<br />

70<br />

60<br />

50<br />

20 μm microwells<br />

Applied voltage = 10 V<br />

Applied voltage = 15 V<br />

Applied voltage = 20 V<br />

Applied voltage = 10 V<br />

Applied voltage = 15 V<br />

Applied voltage = 20 V<br />

30 μm microwells<br />

40<br />

Figure 2: Experimental procedures for particle positioning.<br />

30<br />

20<br />

10<br />

0<br />

0 1 2 3<br />

Number of particles inside a microwell<br />

Figure 5: Particle occupancy of latex particles within the individual microwell<br />

of 20-μm and 30-μm diameter for different applied voltages.<br />

(a)<br />

(b)<br />

Figure 3: The images of micropatterned latex particles in the microwells of (a)<br />

30 and (b) 20 μm in diameter, respectively.<br />

159

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