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Energy Systems and Technologies for the Coming Century ...

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Even though final ethanol yields were found to be ra<strong>the</strong>r low algaes were fur<strong>the</strong>r studiedas a substrate <strong>for</strong> ABE fermentation by Clostridium strains. The advantages of thisfermentation process are (i) that all products (acetone, butanol, ethanol) <strong>for</strong>med during<strong>the</strong> process are valuable (ii) clostridia are able to use many different carbohydrates (bothC6 <strong>and</strong> C5 sugars) (iii) due to cellulolytic <strong>and</strong> xylanolytic activities of <strong>the</strong>se clostridiastrains, direct production of ABE from <strong>the</strong> algae polysaccharides might be possiblewithout enzyme addition.Enzymatic hydrolysis experiments on hydro<strong>the</strong>rmal pretreated (195°C, 10 min, withoutoxygen) U. lactuca <strong>and</strong> C. linum were per<strong>for</strong>med (substrate concentration 5%) bydifferent enzyme mixtures (amylases <strong>and</strong> cellulases) <strong>for</strong> 24 hours. The highest finalglucose content (7 <strong>and</strong> 12 g/l, respectively) was achieved when both amylases <strong>and</strong>cellulases were applied. Liquid fractions of pretreated macroalgaes showed inhibitoryeffect when was fermented by additional glucose, salts, nutrients <strong>and</strong> fermented indifferent dilutions. According to our first results of <strong>the</strong> still ongoing research both C.linum <strong>and</strong> U. lactuca can be suitable substrates <strong>for</strong> ABE fermentation with a total ABEproduction of 0.35 g/g glucose.AcknowledgementThe work is financially supported by <strong>the</strong> PSO Project (<strong>Energy</strong> Production from MarineBiomass: 2008-1-0050).Risø International <strong>Energy</strong> Conference 2011 Proceedings Page 286

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