12th International Conference on Harmful Algae

INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006POSTER ABSTRACTSPO.05-35Liquid chromatography-tandemmass spectrometry techniquesfor the discovery of new marinealgal toxin analogues andmetabolitesSession: PO.05 - Toxin analysisJAB Aasen 1 , MA Quilliam 21 Norwegian School of Veterinary Science,OSLO, Norway2 Institute for Marine Biosciences, NRC,HALIFAX, NOVA SCOTIA, CanadaIn recent years the number ofknown marine algal toxins hasincreased tremendously, mainly bydiscovery of new analogues andmetabolites within known toxingroups. This is due in particular tothe use of LC-MS/MS. In thispresentation, we will outline somenew strategies based on variousMS/MS scanning techniques thatcan be used for comprehensivedetection and identification of newstructural analogues of toxins. Oneexample is the detection of assortednew analogues of the cyclic iminetoxins, spirolides (SPX). Targetingkey collision-induced product ions(m/z 150 or 164) in a precursor ionscanning mode and presenting theion intensity data in a 2-D contourplot (m/z vs. retention time) allowedthe detection of new toxins asindividual 'spots' and alsodetermines their masses. Production scans of those masses thenallow detailed structure informationto be generated. Thus, several newSPX analogues including fatty acidacyl ester metabolites have beenidentified. Examples will also beshown for the yessotoxin (YTX),okadaic acid (OA) and pectenotoxin(PTX) groups. The discovery of allthese new toxin analogues raisessome important questions such astheir toxicological significance andwhether we will be able to monitorfor the toxins without standards.PO.04-12Statewide distribution ofsaxitoxins within Florida pufferfish speciesSession: PO.04 – Food chainsJP Abbott, JH Landsberg, LJFlewelling, L Sebastian, S StahlFlorida Fish and Wildlife Conservation C,ST. PETERSBURG, United States ofAmericaFrom January 2002 to April 2005,28 cases of puffer fish poisoning(PFP) were reported, primarily fromFlorida. A harvesting ban was putinto effect in April 2002. Previouslyunknown in Florida’s marine waters,all incidents were due to saxitoxinpresent in the muscle of southernpuffer fish (Sphoeroides nephelus)originating from the northern IndianRiver Lagoon (IRL) on Florida’sAtlantic coast. Southern puffer fishin the IRL continue to pose asignificant threat to public healthwith maximum levels in the musclejust over 20,106 µg STXeq./100 gtissue (n=384), (action level forshellfish is 80 µg STXeq./100gtissue). Lower levels, ranging from10 to 3133 µg STXeq./100g tissue,have also been found in the muscleof southern puffer fish fromApalachicola (northwest),Jacksonville (northeast), FloridaKeys (south), Charlotte Harbor(southwest), Tampa Bay (centralwest) and Tequesta (southeast).Checkered (S. testudineus) pufferfish from the Florida Keys, IRL andTequesta (range= 17 to 137 µgSTXeq./100g tissue) were generallymuch lower than southern puffers.118