12th International Conference on Harmful Algae

More documents

Recommendations

Info

INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006provides a high degree of selectivityand sensitivity for the determinationof paralytic shellfish poisoning(PSP) toxins (Dell'Aversano et al.,2005). At Fisheries ResearchServices, a HILIC LC-MS methodfor the detection of PSP toxins(STX, NEO, dcSTX, GTX1 to GTX4,dcGTX2 and dcGTX3) has beendeveloped using a singlequadrupole LC-MS for the detectionof these toxins in UK shellfish.Calibration range and linearity wereacceptable (0.5 to 5 µM, R2 > 0.99)and a detection limit of ~0.5 µM wasachieved. Unfortunately, sensitivitywas at least ten times lessfavourable when compared withvalidated high performance-liquidchromatography with fluorescencedetection (HPLC-FLD), which canbe prone to interference fromendogenous fluorescent matrixcompounds. In order to increasesensitivity of the LC-MS, we arecurrently developing methods forimproved sample preparation, usingsolid phase extraction (SPE) andwe will present our preliminaryfindings.Dell'Aversano et al. / J. Chromatogr.A (2005) 190-201O.07-05Anatoxin contamination offreshwater resources in NewZealandSession: O.07 - Toxin analysis 1Presentation time: 12:20 - 12:40PT Holland 1 , AI Selwood 1 , SA Wood 1 ,K Smith 1 , P McNabb 1 , P Rasmussen 21 Cawthron Institute, NELSON, NewZealand2 Australian Water Quality Centre,BOLIVAR, SA, AustraliaCyanobacterial blooms in NewZealand water resources have beensurveyed and, in response to strictnew standards for drinking water,more intensive monitoring forcyanotoxins has been initiated. Afilamentous bloom-formingcyanobacterium from a lake hasbeen shown to be a potent producerof anatoxin-a. Phylogenetic analysisof the 16S rRNA and rpoB genesequences classified the organismas Aphanizomenon issatschenkoi.Benthic samples of Oscillatoria sp.and Phormidium sp. from severalrivers were shown to produceanatoxin-a and homoanatoxin-a,and were conclusively associatedwith illness and death of dogs.These findings are the firstconfirmed reporting of anatoxinproducingspecies in the SouthernHemisphere and the first report ofanatoxin-a production by A.issatschenkoi. An isolate of thelatter was brought into culture and,during LC-MS assays for anatoxin-aproduction, an unstable biosyntheticprecursor was found in freshsamples of the culture.Characterisation of the precursor isdescribed and implications foraccurate analysis of anatoxin-a inwater samples are discussed. TheNew Zealand Drinking WaterStandards (2005) includeprovisional maximum allowablevalues (PMAVs) for anatoxin-a,homoanatoxin-a,cylindrospermopsin andmicrocystins of 6, 2, 1 and 1 µg/Lrespectively. The validation ofsuitable test methods to enforcethese low limits is presented.O.07-06The Biosense ASP ELISA - anearly warning tool for theenvironmental monitoring ofdomoic acid in phytoplanktonand seawaterSession: O.07 - Toxin analysis 143
INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006Presentation time: 12:40 - 13:00H Kleivdal 1 , SI Kristiansen 1 , CCampbell 2 , K Davidson 21 Biosense Laboratories AS, BERGEN,Norway2 Scottish Association for Marine Science,OBAN, United KingdomThe monitoring of potential harmfulalgae in coastal waters often relieson species determination and anestimation of their respective cellcounts. There is no clear correlationbetween toxin levels and cellnumbers for many toxin-producingspecies, and it is difficult to predictthe effects of an upcoming bloom assome algae may be harmful at lowabundance. A parallel routinedetermination of actual toxin levelsis therefore important tocomplement the phytomonitoring aspart of an effective early warningsystem. Until recently, the analysisof toxins in phytoplankton has beenperformed in centralizedlaboratories with costly andadvanced instrumentation far fromthe sampling sites. As simple rapidassays for marine biotoxins becomeavailable, the analysis ofphytoplankton and seawatersamples can be performed close tothe sampling site and provide a 'realtime' monitoring of the toxin level incoastal waters. We have evaluatedthe Biosense ASP ELISA for directanalysis of domoic acid (DA) inseawater and several cultured andnatural Pseudo-nitzschia sp.samples, using a simple samplepreparation protocol. The ASPELISA allows precise and accuratequantitation of DA at lowconcentrations in particulate anddissolved fractions, with anestimated limit of detection at 50pg/mL.O.08-01Domoic acid production is notlinked to silicate limitation innatural populations of PseudonitzschiaSession: O.08 - Population dynamics 2Presentation time: 11:00 - 11:20WP Cochlan 1 , ML Wells 2 , VL Trainer 3 ,CG Trick 4 , EJ Lessard 5 , BM Hickey 51 Romberg Tiburon Center, SFSU,TIBURON, United States of America2 University of Maine, ORONO, UnitedStates of America3 Northwest Fisheries Science Center,NOAA, SEATTLE, United States ofAmerica4 Department of Biology, University of Wes,LONDON, Canada5 University of Washington, SEATTLE,United States of AmericaA central paradigm in the study oftoxigenic diatoms is that silicatelimitation induces intense cellularproduction of domoic acid. Wetested this hypothesis during a 2004ECOHAB-PNW cruise in the coastalwaters off Washington State andBritish Columbia where toxigenicPseudo-nitzschia typically occur.Our results show that the highestlevels of cellular toxin (5-64 pg/cell)correlate poorly with ambient silicateconcentrations and occur wheredissolved silicate concentrations are5-50 µM. None of the ~400particulate analyses showedelevated cellular toxinconcentrations at < 4 µM Si. Theseconcentrations are well above thatconsidered limiting for Si uptake andgrowth of most neritic diatoms.Cellular toxin levels also did notcorrelate with N or P concentrations,indicating that toxin production in Ispp. is not governed bymacronutrient availability. A similarresult was found during the intensetoxic Pseudo-nitzschia bloom inMonterey Bay, 1998. The mostestablished correlate for elevateddomoic acid levels in 2004 was the44
Page 1 and 2: INTERNATIONAL SOCIETYFOR THE STUDY
Page 3 and 4: Dear participantWelcome to Denmark
Page 5 and 6: INTERNATIONAL SOCIETY FOR THE STUDY
Page 43: INTERNATIONAL SOCIETY FOR THE STUDY
Page 95 and 96:
INTERNATIONAL SOCIETY FOR THE STUDY
Page 97 and 98:
Page 99 and 100:
Page 101 and 102:
Page 103 and 104:
Page 105 and 106:
Page 107 and 108:
Page 109 and 110:
Page 111 and 112:
Page 113 and 114:
Page 115 and 116:
Page 117 and 118:
Page 119 and 120:
Page 121 and 122:
Page 123 and 124:
Page 125 and 126:
Page 127 and 128:
Page 129 and 130:
Page 131 and 132:
Page 133 and 134:
Page 135 and 136:
Page 137 and 138:
Page 139 and 140:
Page 141 and 142:
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
Page 149 and 150:
Page 151 and 152:
Page 153 and 154:
Page 155 and 156:
Page 157 and 158:
Page 159 and 160:
Page 161 and 162:
Page 163 and 164:
Page 165 and 166:
Page 167 and 168:
Page 169 and 170:
Page 171 and 172:
Page 173 and 174:
Page 175 and 176:
Page 177 and 178:
Page 179 and 180:
Page 181 and 182:
Page 183 and 184:
Page 185 and 186:
Page 187 and 188:
Page 189 and 190:
Page 191 and 192:
Page 193 and 194:
Page 195 and 196:
Page 197 and 198:
Page 199 and 200:
Page 201 and 202:
Page 203 and 204:
Page 205 and 206:
Page 207 and 208:
Page 209 and 210:
Page 211 and 212:
Page 213 and 214:
Page 215 and 216:
Page 217 and 218:
Page 219 and 220:
Page 221 and 222:
Page 223 and 224:
Page 225 and 226:
Page 227 and 228:
Page 229 and 230:
Page 231 and 232:
Page 233 and 234:
Page 235 and 236:
Page 237 and 238:
Page 239 and 240:
Page 241 and 242:
Page 243 and 244:
Page 245 and 246:
Page 247 and 248:
Page 249 and 250:
Page 251 and 252:
Page 253 and 254:
Page 255 and 256:
Page 257 and 258:
Page 259 and 260:
Page 261 and 262:
Page 263 and 264:
Page 265 and 266:
Page 267 and 268:
Page 269 and 270:
Page 271 and 272:
Page 273 and 274:
Page 275 and 276:
Page 277 and 278:
Page 279 and 280:
Page 281 and 282:
Page 283 and 284:
Page 285 and 286:
Page 287 and 288:
Page 289 and 290:
Page 291 and 292:
Page 293 and 294:
Page 295 and 296:
Page 297 and 298:
Page 299 and 300:
Page 301 and 302:
Page 303 and 304:
Page 305 and 306:
Page 307 and 308:
Page 309 and 310:
Page 311 and 312:
Page 313 and 314:
Page 315 and 316:
Page 317 and 318:
Page 319 and 320:
Page 321 and 322:
Page 323 and 324:
Page 325 and 326:
Page 327 and 328:
Page 329 and 330:
Page 331 and 332:
Page 333 and 334:
Page 335 and 336:
Page 337 and 338:
Page 339 and 340:
Page 341 and 342:
Page 343 and 344:
Page 345 and 346:
Page 347 and 348:
Page 349 and 350:
Page 351 and 352:
Page 353 and 354:
Page 355 and 356:
Page 357 and 358:
Page 359 and 360:
Page 361 and 362:
Page 363 and 364:
Page 365 and 366:
Page 367 and 368:
Page 369 and 370:
Page 371 and 372:
Page 373 and 374:
Page 375 and 376:
Page 377 and 378:
Page 379 and 380:
Page 381 and 382:
Page 383 and 384:
Page 385 and 386:
Page 387 and 388:
Page 389 and 390:
Page 391 and 392:
Page 393 and 394:
Page 395 and 396:
Page 397 and 398:
Page 399 and 400:
Page 401 and 402:
Page 403 and 404:
show all

12th International Conference on Harmful Algae

Create successful ePaper yourself

Delete template?

Save as template?