12th International Conference on Harmful Algae

INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006donghaiense, and that N-phenyl-2-naphthylamine and linoleic acidmight play an important role in theinhibition.PO.05-44Production of monoclonalantibody and development of anenzyme-linked immunosorbentassay for the determination ofokadaic acid in shellfishSession: PO.05 - MonitoringRen-Yan Liu 1 , Bing-Jun Chen 2 , Dao-Yan Xu 3 , Yu-Bo Liang 4 , Bing Liang 31 Dalian Maritime University, DALIAN, China2 Dalian Fisheries University, DALIAN,China3 Natl. Marine Environ. Monitoring Centre,DALIAN, China4 SOA Key Lab of Coastal Env. & EcosysRes, DALIAN, ChinaAn indirect competitive enzymelinkedimmunosorbent assay forquantitation of okadaic acid(OA), amarine biotoxin associated redtides, was developed by preparationof a monoclonal antibody againstOA using the cell-fusing method.OA-KLH was used as immunogen,and the spleen cells of immunizedmice were fused with Sp2/O cells.Clones secreting specificmonoclonal antibodies werescreened by indirect ELISA usingOA-OVA as coating ligand. Aftercloning, three hybridoma cell clonesstably producing anti-OAmonoclonal antibody were obtained.Detection of OA concentrations byindirect competitive ELISA wasestablished. The detection limit ofOA was 0.781ng/ml. The level ofOA in shellfish from China wasdetermined using the idc-ELISAmethod. A good correlation(R=0.7963,P‹ 0.001) was observedbetween idc-ELISA and HPLC-MS/MS.PO.10-28Response to small-scaleturbulence by naturalmicrophytoplanktonassemblages along a naturalAlexandrium minutum bloomeventSession: PO.10 - Ecophysiology &autecologyG Llaveria, E Garcés, E Berdalet, NSampedro, S Anglès, Ò GuadayolInstitut de Ciències del Mar, BARCELONA,SpainSmall-scale turbulence is anenvironmental factor that maydirectly affect different physiologicalprocesses of phytoplankton. Mostavailable data have been generatedin the laboratory using monospecificphytoplankton cultures. In thepresent work we used field samplesof a bloom of dinoflagellatesdominated by Alexandriumminutum, Scrippsiella spp. andProrocentrum micans, in differentphases of the succession, and wetested their sensitivity toexperimentally generated smallscaleturbulence.Three experiments, each 15 daysapart, were performed on themicroplankton fraction (15-60 µm) ofthe natural water. The sampleswere distributed in 4-liter sphericalflasks and maintained still orexposed to turbulence generated byan orbital shaker (turbulentdissipation rate epsilon~15 cm 2 s -3 )for ca. 10 days. The response wasevaluated in terms of biomass yield,net growth rate and cyst abundanceof the dominant species.Diatoms, A. minutum andScrippsiella spp. collapsed understill conditions while P. micansgrowth was not favoured byturbulence. Temporary cystformation of A. minutum was224