12th International Conference on Harmful Algae

INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 200610 7 cells/l). There were alsosporadic brown tides due to intensegrowth of Fibrocapsa japonicaduring summer. In bloom periodicityand cell abundance, spring andsummer were the most criticalperiods for toxicity events. In fact,HPLC and electrochemical immunosensoranalyses indicated thepresence of domoic acid in naturalphytoplankton populations testedover a full year; using LC-MS/MSmethods a palytoxin analogue wasalso detected in mucous aggregatesdominated by Ostreopsis ovata.PO.15-35Functionalized nanoparticles anddielectrophoretic detection ofharmful algaeSession: PO. 15 - MonitoringLaurie Connel, J. Duy, R. SmithUniversity of Maine, ORONO, UnitedStates of AmericaA developing trend in harmful algaedetection is microfabricated sensorsand instruments that utilizeelectronic sensing mechanisms,which can reduce the size andpower requirements, and potentiallyincrease the efficiency of datageneration, collection andprocessing through circuitintegration. Water quality managers,researchers and public healthofficials require timely and accuratedetection of harmful species in acost effective manner. Therefore,there is a need for improvedmethods capable of rapid on-siteanalysis. The use of a DNA analog,peptide nucleic acids (PNAs), withtheir unique binding parameters, isused to decrease target bindingtime and allow hybridization at verylow salt concentrations. HABspecific PNA probes are bound tofunctionalized gold nanoparticles.The addition of the functionalizednanoparticles to the target solutionis used to capture and concentratetarget molecules as well as form anelectrical bridge in nanotubes.These bridges are then detected viameasurement of electricalimpedance across the tube and thedata collected. We present hereour approach and preliminary data.PO.13-59A culture collection of harmfulmarine microalgae in BrazilSession: PO.13 - Regional eventsL.C. Coutinho 1 , E. Barbarino 2 , S.O.Lourenço 21 Universidade Santa Úrsula, RIO DEJANEIRO, Brazil2 Universidade Federal Fluminense,NITEROI, BrazilThe interest for cultivation ofmicroalgae has been increasing inBrazil due to the growth ofaquaculture and research onenvironmental sciences, includingtests of ecotoxicology, which reflecta new demand for the evaluation ofimpact of pollutants. Studies onharmful microalgae are alsorelevant. After a 75-year phase ofdescriptive work, including recordsof algal blooms, since the 1990sBrazilian experts have been alsorunning analytical and experimentalstudies on harmful microalgae.However, the small number ofpotentially harmful strains in the ca.40 Brazilian culture collections ofmicroalgae creates a bottleneck tothe development of newapproaches for studies on harmfulmicroalgae in the country. Theongoing expansion of the'Elizabeth Aidar' Microalgal CultureCollection (Fluminense FederalUniversity, Rio de Janeiro State),represents the start of theorganisation of a national reference154