12th International Conference on Harmful Algae

INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006management of bloom situationsare important issues to managers ofbathing waters and drinking waterreservoirs worldwide. Anoperational approach formanagement would be to model theblooms; thus being able to predictmass occurrences and evaluatevarious measures to reduce thebloom intensity and frequency. Inthe present study, a dynamic modelhas been established for a smallDanish lake using the model toolECO Lab. The objective was toidentify the most importantparameters that must be taken intoaccount when setting up suchmodels. The model describes thedynamics of the most importantalgal groups includingcyanobacteria, using a dynamicdescription of the hydrodynamics ofthe lakes, weather conditions andnutrient loading. The model iscalibrated against monitoring data.A first attempt was made to includepredictions of the toxicity in variouscompartments: intracellular, inwater, in sediment.PO.15-12Development of a comprehensivemethod for monitoring harmfulalgae using real-time PCR assaySession: PO.15 - MonitoringRyoma Kamikawa 1 , J Asai 1 , TMiyahara 2 , K Murata 3 , K Ohyama 4 , SYoshimatsu 4 , T Yoshida 5 , Y Sako 11 Kyoto University, KYOTO, Japan2 Fisheries Research Center of Oita Pref.,OITA, Japan3 Kagoshima Fisheries Technology Center,KAGOSHIMA, Japan4 Akashiwo Research Institute of Kagawa,KAGAWA, Japan5 Fukui Prefectural University, FUKUI,JapanIn Japan, several harmful algaeform red tides and often causemass mortality of cultured fish andshellfish. In order to minimize thedamage to the fisheries industry, itis necessary to monitor thecausative harmful algae rapidly,sensitively, and comprehensively. Inthis study, we developed a sensitivemonitoring method by using realtimePCR (qPCR) assay againstthree dinoflagellates, namelyCochlodinium polykrikoides,Heterocapsa circularisquama, andKarenia mikimotoi, and tworaphidophycean flagellates, namelyChattonella spp. and Heterosigmaakashiwo. The assay contains anefficient DNA extraction protocolfrom these harmful algae, andtherefore DNA extraction for qPCRis necessary only once formonitoring of the algae. Detectionand quantification with qPCR,where the detection sensitivity wasonly 1 cell for all targeted species,were not affected by growth stagesand the presence of non-targetedspecies. QPCR assay also labelledthe harmful algae in environmentalsamples, where no targeted specieswas found by direct counting.Therefore, this comprehensiveassay will be a powerful tool forprecise prediction of the occurrenceof harmful algae.PO.08-08Effects of cyanobacteria oncopepod egg production in theGulf of Finland, Baltic SeaSession: PO.08 - ToxicologyMR Karjalainen, E Lindén, S Viitasalo,M ViitasaloFinnish Institute of Marine Research,HELSINKI, FinlandCyanobacterial blooms occur in theBaltic Sea every summer. Copepodpeak abundance overlaps with thecyanobacteria mass occurrences.Copepods, in turn, are the main202