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12th International Conference on Harmful Algae

12th International Conference on Harmful Algae

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INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <str<strong>on</strong>g>Internati<strong>on</strong>al</str<strong>on</strong>g> <str<strong>on</strong>g>C<strong>on</strong>ference</str<strong>on</strong>g> <strong>on</strong> <strong>Harmful</strong> <strong>Algae</strong>, Copenhagen, Denmark, 4-8 September 2006Gambierdiscus toxicus were grownin N- (nitrate, amm<strong>on</strong>ium, freeamino acids, urea, or putrescine) orP-limited (phosphate, ß-glycerophosphate, or nucleotides)xenic batch cultures. Growth ratesranged from 0.11- 0.18 d -1 except<strong>on</strong> urea (no growth). Cellular N andP pools were uncoupled fromc<strong>on</strong>diti<strong>on</strong>s in the water and celldivisi<strong>on</strong> c<strong>on</strong>tinued after the limitingnutrient was below detectable limits.N:P varied from 3-34 (1651) and 2-37 (strain 1655) with no relati<strong>on</strong>shipbetween cellular N:P and totaltoxicity. Strain 1655 was roughlyfive-fold more toxic than strain 1651.The N source had no effect <strong>on</strong>toxicity in either strain. The Psource had no effect <strong>on</strong> toxicity instrain 1651, but strain 1655 cellsgrown <strong>on</strong> ß-glycerophosphate weremore toxic. Stati<strong>on</strong>ary phase cellswere more toxic than exp<strong>on</strong>entialphase cells in both strains underboth N- and P-limitati<strong>on</strong>. In P-limited semi-c<strong>on</strong>tinuous cultures,the toxicity of strain 1655 decreased(43.7-79.3 fg C-Ctx1Eq cell -1 ) as thegrowth rate increased (0.02-0.15 d -1 ). Toxicity differences were afuncti<strong>on</strong> of growth phase (batchculture) or growth rate (semic<strong>on</strong>tinuousculture). Nutrient sourceand type of nutrient limitati<strong>on</strong> havinglittle to no effect.PO.13-58Involvement of cyanobacteria inthe tropical ecotoxicologicalphenomen<strong>on</strong> of ciguatera fishpois<strong>on</strong>ingSessi<strong>on</strong>: PO.13 - Regi<strong>on</strong>al eventsD Laurent, AS Kerbrat, I de Fremicourt,T Darius, M Chinain, S PauillacIRD, NOUMEA, New Caled<strong>on</strong>iaDuring 2005, a cyanobacterialproliferati<strong>on</strong> (Hydrocoleumlyngbyaceum) was found to inducea severe ciguatera outbreak in Lifou(Loyalty Islands, New Caled<strong>on</strong>ia).Lipid-soluble extracts ofcyanobacteria and giant clams werefound to c<strong>on</strong>tain CTX-likecompounds, as assessed byneuroblastoma cell bioassays andcompetitive membrane-bindingassay using tritiated brevetoxin-3.According to the mouse bioassay,water-soluble extracts of bothsamples appear to c<strong>on</strong>tain the wellknownalkaloid neurotoxinsproduced by cyanobacteria andresp<strong>on</strong>sible for Paralytic ShellfishPois<strong>on</strong>ing (PSP).This is the first dem<strong>on</strong>strati<strong>on</strong> ofsimultaneous occurrence of PSPtoxins and CTX-like toxins inextracts of marine cyanobacteria.The chemical identificati<strong>on</strong> of thesetoxins is currently underway byliquid chromatography coupled withmass spectrometry (LC/MS).Although l<strong>on</strong>g-term observati<strong>on</strong>s willbe necessary to decipher the trendsin dinoflagellate and cyanobacteriapopulati<strong>on</strong>s as well as thefavourable envir<strong>on</strong>mentalparameters, the new findingsrequire that CFP risk assessmentprograms must now includem<strong>on</strong>itoring of cyanobacteria besidesthe usual screening fordinoflagellates. In the perspective ofglobal warming, such events wouldprobably arise more frequently.PO.11-02Effect of bicarb<strong>on</strong>ate additi<strong>on</strong> <strong>on</strong>allelopathy in OscillatoriaagardhiiSessi<strong>on</strong>: PO.11 – AllelopathyLA Lawt<strong>on</strong> 1 , GA Akin-Oriola 2 ,1 The Robert Gord<strong>on</strong> University,ABERDEEN, United Kingdom2 Lagos State University, LAGOS, Nigeria215

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