12th International Conference on Harmful Algae

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INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006complex cell communication. Theyare synthesized by successivecondensations of acetate units viaactivity of enzymes known aspolyketide synthases (PKSs).Bacteria, fungi and plants mayproduce PKS but Type I modularform was formerly known only forbacteria and fungi. DNA sequenceswere used to identify modular TypeI PKS genes within genomes ofprotists belonging to theevolutionary lineages of alveolatesand amoebozoa. We present thatthe newly sequenced genomes ofrepresentatives of other protistangroups, the chlorophytesOstreococcus tauri andChlamydomonas reinhardtii, and thehaptophyte Emiliana huxleyi, as wellas expressed sequence tags(ESTs) of various harmfulmicroalgal species also containputative PKS genes. Bioinformaticanalysis reveals that these protistanPKS genes cluster into a uniqueclade. These observations provideimportant insight into the evolutionof polyketide metabolic pathwaysand suggest that PKS enzymesfound in various lineages representa conserved ancestral form derivedfrom early eukaryotes.PO.02-03Molecular investigations on thetoxic marine dinoflagellateAlexandrium minutumSession: PO.02 - GenomicsI Jung 1 , U John 1 , G Glöckner 2 , UTillmann 1 , B Krock 1 , AD Cembella 11 Alfred Wegener Institute, Bremerhaven,BREMERHAVEN, Germany2 Fritz Lipmann Institute for Age Research,JENA, GermanyAlexandrium minutum is a widelydistributed HAB dinoflagellate thattypically produces PSP neurotoxins(gonyautoxins 1,2,3,4), as well aspoorly characterizedallelochemicals. Within the EU-Project ESTTAL (ExpressedSequence Tags of Toxic Algae), weaim to identify candidate genes andprocesses putatively involved intoxicity and allelochemicalinteractions, and in growthregulation and stress responses. Toobtain RNA samples linked withphysiological status, batch culturesof A. minutum were grown underdifferent environmental conditions,yielding differences in growth, PSPtoxin production, and allelochemicalpotency. As a basis for geneexpression analysis, we generateda normalized cDNA library, fromwhich about 10,000 expressedsequence tags (ESTs) weregenerated. Annotation of these datayielded first insights into thegenome of A. minutum. Based onthe EST library, we designed anoligonucleotide microarray, whichwill be used to screen fordifferences in gene expressionamong the differently treated A.minutum cultures. Here we presentthe results of the EST libraryanalysis, the design of themicroarray, and physiological dataof cultures grown for geneexpression analysis.PO.06-24Dynamic modelling ofcyanobacterial blooms in lakesusing ECO LabSession: PO.06 - Population dynamicsH Kaas 1 , A CHR Erichsen 1 , P Stæhr 21 DHI Water & Environment, HØRSHOLM,Denmark2 Freshwater Biological Laboratory;University of Copenhagen,COPENHAGEN, DenmarkCyanobacterial blooms are wellknown to produce toxins that pose ahealth threat to humans. Therefore201
INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006management of bloom situationsare important issues to managers ofbathing waters and drinking waterreservoirs worldwide. Anoperational approach formanagement would be to model theblooms; thus being able to predictmass occurrences and evaluatevarious measures to reduce thebloom intensity and frequency. Inthe present study, a dynamic modelhas been established for a smallDanish lake using the model toolECO Lab. The objective was toidentify the most importantparameters that must be taken intoaccount when setting up suchmodels. The model describes thedynamics of the most importantalgal groups includingcyanobacteria, using a dynamicdescription of the hydrodynamics ofthe lakes, weather conditions andnutrient loading. The model iscalibrated against monitoring data.A first attempt was made to includepredictions of the toxicity in variouscompartments: intracellular, inwater, in sediment.PO.15-12Development of a comprehensivemethod for monitoring harmfulalgae using real-time PCR assaySession: PO.15 - MonitoringRyoma Kamikawa 1 , J Asai 1 , TMiyahara 2 , K Murata 3 , K Ohyama 4 , SYoshimatsu 4 , T Yoshida 5 , Y Sako 11 Kyoto University, KYOTO, Japan2 Fisheries Research Center of Oita Pref.,OITA, Japan3 Kagoshima Fisheries Technology Center,KAGOSHIMA, Japan4 Akashiwo Research Institute of Kagawa,KAGAWA, Japan5 Fukui Prefectural University, FUKUI,JapanIn Japan, several harmful algaeform red tides and often causemass mortality of cultured fish andshellfish. In order to minimize thedamage to the fisheries industry, itis necessary to monitor thecausative harmful algae rapidly,sensitively, and comprehensively. Inthis study, we developed a sensitivemonitoring method by using realtimePCR (qPCR) assay againstthree dinoflagellates, namelyCochlodinium polykrikoides,Heterocapsa circularisquama, andKarenia mikimotoi, and tworaphidophycean flagellates, namelyChattonella spp. and Heterosigmaakashiwo. The assay contains anefficient DNA extraction protocolfrom these harmful algae, andtherefore DNA extraction for qPCRis necessary only once formonitoring of the algae. Detectionand quantification with qPCR,where the detection sensitivity wasonly 1 cell for all targeted species,were not affected by growth stagesand the presence of non-targetedspecies. QPCR assay also labelledthe harmful algae in environmentalsamples, where no targeted specieswas found by direct counting.Therefore, this comprehensiveassay will be a powerful tool forprecise prediction of the occurrenceof harmful algae.PO.08-08Effects of cyanobacteria oncopepod egg production in theGulf of Finland, Baltic SeaSession: PO.08 - ToxicologyMR Karjalainen, E Lindén, S Viitasalo,M ViitasaloFinnish Institute of Marine Research,HELSINKI, FinlandCyanobacterial blooms occur in theBaltic Sea every summer. Copepodpeak abundance overlaps with thecyanobacteria mass occurrences.Copepods, in turn, are the main202
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Dear participantWelcome to Denmark
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12th International Conference on Harmful Algae

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