12th International Conference on Harmful Algae

More documents

Recommendations

Info

INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 2006and their prey are difficult to monitorusing standard microscopy. Thehigh magnification needed todistinguish between HAB and preyspecies results in shallow depths offield and prevents tracking the 3-dimensional paths of multipleswimming organisms. Microscopicdigital holography overcomes thislimitation by using numericalreconstruction to provide in-focusviews of all the organisms within a 3mm depth. The accuracy in thetracking procedure is sufficient toprovide fully 3-dimensionaltrajectories from one view. Thetechnique is applied to cultures oftoxic and nontoxic strains ofKarlodinium veneficum with andwithout a predator, Oxyrrhis marina,as well as Pfiesteria piscicida withand without its algal prey,Rhodomonas. Typical swimmingbehaviors include helical swimmingand conspecifics revolving aroundeach other. Karlodinium individualsare observed to have swimmingspeeds ranging from 0.05 to 0.5mm/s and Pfiesteria cells 0.1-1mm/s. We also observe a changein the average swimming speed ofP. piscicida from 0.5 mm/s inisolation to 0.9 mm/s in thepresence of its prey, Rhodomonas.PO.16-06Effects of temperature and lighton benthic cell germination andgerminated cell survival of thenoxious raphidophyteHeterosigma akashiwoSession: PO.16 – Life cyclesTomoyuki Shikata 1 , S Nagasoe 2 , TMatsubara 2 , Y Yamasaki 2 , YShimasaki 2 , Y Oshima 2 , T Honjo 2FUKUOKA, Japan2 Graduate School, Kyushu University,FUKUOKA, JapanThe effects of temperature and lighton germination of benthic cells andsurvival of the germinated motilecells of Heterosigma akashiwo wereexamined with bottom sedimentsincluding the benthic cells of thisorganism. The motile cellsappeared in a temperature rangefrom 5 °C to 30 °C in suspensionsof mixed sediment and seawaterwithin three weeks, but attemperatures ≦12 °C, the cellnumbers were markedly low and theappearance was delayed. When thesamples, incubated at eachtemperature, were incubatedat 20°C, only few motile cells appeared.In suspensions incubated in the lightor dark, despite counting motilecells at six-hourly intervals, thenumber of motile cells germinated inthe dark was significantly lower thanthose in the light. Subsequently,when the suspension incubated inthe dark was exposed to light, onlya few newly germinated motile cellswere observed. These resultsindicate that germination of thebenthic cells is independent oftemperature and light if thesediment is suspended intoseawater, but the speed of thegermination process depends ontemperature, and survival of thecells just after germination isstrongly affected by temperatureand light.PO.15-27Development of a simple andsensitive monitoring method forthe shellfish-killing dinoflagellateHeterocapsa circularisquamausing real-time PCR assaySession: PO.15 - MonitoringT Shiraishi 1 , R Kamikawa 1 , Y Sako 1 , STaino 2 , Y Hayashi 2 , I Imai 11 Kyoto University, KYOTO, Japan279
INTERNATIONAL SOCIETY FOR THE STUDY OF HARMFUL ALGAE12 th <strong>International</strong> <strong>Conference</strong> on Harmful Algae, Copenhagen, Denmark, 4-8 September 20062 Kochi Pref. Fish. Experimental Station,KOCHI, JapanHeterocapsa circularisquama is themost noxious red tide dinoflagellatealong the Japanese coasts, causingmass mortalities of both natural andcultured bivalves. It is indispensablefor mitigating the negative impactsto monitor this species rapidly,easily, and sensitively. Real-timePCR assay is a sensitive andspecific method for the detectionand quantification of microalgae,however, procedures for real-timePCR assay are not practical for fieldmonitoring. In this study, wedeveloped a simple and sensitivemonitoring method for H.circularisquama using real-timePCR assay. Quantitative DNAextraction was made by boiling thefilter in TE (Tris-HCl and EDTA)buffer after concentrating the cellson the Nuclepore filter. This assaymade specific and quantitativedetection possible even with theabundant presence of other algae.Enumeration of cells in naturalsamples revealed identical resultsby real-time PCR assay and theindirect fluorescent antibodytechnique even at cell densities aslow as 1 cell per liter. Hence, thismethod is a powerful and feasibletool for monitoring of H.circularisquama. The work nowcontinues using this method tounderstand population dynamics.PO.01-03Harmful algae can be transportedvia relocation of bivalve shellfishSession: PO.01 - GeneticsSE Shumway 1 , HT Hégaret 1 , GHWikfors 21 University of Connecticut, GROTON,United States of America2 NOAA-NMFS, MILFORD, CT 06460,United States of AmericaOur study tested the hypothesis thatharmful algae can be introducedinto new environments by means ofshellfish relocations, a commonpractice for commercially-exploitedbivalve molluscs. We identifiedwhich managed shellfish speciesand HABs co-occur geographicallyand established a protocol toassess the potential of the bivalvespecies to be vectors for transportof harmful algae. Cultured strains ofharmful algae, Alexandriumfundyense, Heterosigma akashiwo,Prorocentrum minimum, andGymnodinium mikimotoi were fed tobivalve molluscs for two days at anatural bloom concentration toassess the ability of the algal cellsto pass intact through the digestivetract and subsequently grow. Afterfeeding, the bivalves were kept fortwo days in ultrafiltered seawater.Biodeposits were collected andobserved under the microscopeafter 24 and 48 h to evaluate thepresence or absence of intact,viable cells or temporary cysts ofthe algae. Subsamples ofbiodeposits were transferred intoboth algal culture medium andfiltered seawater and monitoredmicroscopically for algal growth.Intact algal cells of the variousharmful algae were seen inbiodeposits and generally these reestablishedgrowing populations.PO.10-42Phosphatase activity in PfiesteriashumwayaeSession: PO.10 - Ecophysiology &autecologyHM Skelton 1 , MW Parrow 2 , JMBurkholder 11 North Carolina State University, RALEIGH,United States of America2 University of North Carolina,CHARLOTTE, United States of America280
Page 1 and 2:
INTERNATIONAL SOCIETYFOR THE STUDY
Page 3 and 4:
Dear participantWelcome to Denmark
Page 5 and 6:
INTERNATIONAL SOCIETY FOR THE STUDY
Page 7 and 8:
Page 9 and 10:
Page 11 and 12:
Page 13 and 14:
Page 15 and 16:
Page 17 and 18:
Page 19 and 20:
Page 21 and 22:
Page 23 and 24:
Page 25 and 26:
Page 27 and 28:
Page 29 and 30:
Page 31 and 32:
Page 33 and 34:
Page 35 and 36:
Page 37 and 38:
Page 39 and 40:
Page 41 and 42:
Page 43 and 44:
Page 45 and 46:
Page 47 and 48:
Page 49 and 50:
Page 51 and 52:
Page 53 and 54:
Page 55 and 56:
Page 57 and 58:
Page 59 and 60:
Page 61 and 62:
Page 63 and 64:
Page 65 and 66:
Page 67 and 68:
Page 69 and 70:
Page 71 and 72:
Page 73 and 74:
Page 75 and 76:
Page 77 and 78:
Page 79 and 80:
Page 81 and 82:
Page 83 and 84:
Page 85 and 86:
Page 87 and 88:
Page 89 and 90:
Page 91 and 92:
Page 93 and 94:
Page 95 and 96:
Page 97 and 98:
Page 99 and 100:
Page 101 and 102:
Page 103 and 104:
Page 105 and 106:
Page 107 and 108:
Page 109 and 110:
Page 111 and 112:
Page 113 and 114:
Page 115 and 116:
Page 117 and 118:
Page 119 and 120:
Page 121 and 122:
Page 123 and 124:
Page 125 and 126:
Page 127 and 128:
Page 129 and 130:
Page 131 and 132:
Page 133 and 134:
Page 135 and 136:
Page 137 and 138:
Page 139 and 140:
Page 141 and 142:
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
Page 149 and 150:
Page 151 and 152:
Page 153 and 154:
Page 155 and 156:
Page 157 and 158:
Page 159 and 160:
Page 161 and 162:
Page 163 and 164:
Page 165 and 166:
Page 167 and 168:
Page 169 and 170:
Page 171 and 172:
Page 173 and 174:
Page 175 and 176:
Page 177 and 178:
Page 179 and 180:
Page 181 and 182:
Page 183 and 184:
Page 185 and 186:
Page 187 and 188:
Page 189 and 190:
Page 191 and 192:
Page 193 and 194:
Page 195 and 196:
Page 197 and 198:
Page 199 and 200:
Page 201 and 202:
Page 203 and 204:
Page 205 and 206:
Page 207 and 208:
Page 209 and 210:
Page 211 and 212:
Page 213 and 214:
Page 215 and 216:
Page 217 and 218:
Page 219 and 220:
Page 221 and 222:
Page 223 and 224:
Page 225 and 226:
Page 227 and 228:
Page 229 and 230: INTERNATIONAL SOCIETY FOR THE STUDY
Page 279: INTERNATIONAL SOCIETY FOR THE STUDY
Page 331 and 332:
Page 333 and 334:
Page 335 and 336:
Page 337 and 338:
Page 339 and 340:
Page 341 and 342:
Page 343 and 344:
Page 345 and 346:
Page 347 and 348:
Page 349 and 350:
Page 351 and 352:
Page 353 and 354:
Page 355 and 356:
Page 357 and 358:
Page 359 and 360:
Page 361 and 362:
Page 363 and 364:
Page 365 and 366:
Page 367 and 368:
Page 369 and 370:
Page 371 and 372:
Page 373 and 374:
Page 375 and 376:
Page 377 and 378:
Page 379 and 380:
Page 381 and 382:
Page 383 and 384:
Page 385 and 386:
Page 387 and 388:
Page 389 and 390:
Page 391 and 392:
Page 393 and 394:
Page 395 and 396:
Page 397 and 398:
Page 399 and 400:
Page 401 and 402:
Page 403 and 404:
show all

12th International Conference on Harmful Algae

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?